Skip to the beginning of the images gallery

NT-proBNP Mouse mAb - Primary antibody, for ELISA,CLIA,GICA,Detection antibody,FIA various applications, from Human, generated in Mouse, specific to NT-proBNP, IgG1

8.66mg/mL

Application:

CLIA
Detection Antibody
ELISA
FIA
GICA

Features and benefits

Host species: Mouse
Species reactivity(Reacts with): Human
Isotype: Mouse IgG1
Clone Number: 1H2
Conjugation: Unconjugated

Item Number

Ab007709

Grouped product items
SKU	Size	Availability	Price
Ab007709-10μg	10μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$26.90
Ab007709-50μg	50μg	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$69.90
Ab007709-100μg	100μg	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$105.90
Ab007709-1mg	1mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$774.90

mAb(1H2); Mouse anti Human NT-proBNP antibody; Detection antibody, ELISA, CLIA, GICA, FIA; Unconjugated

View related series

Click to view target information: GCSAM Click to view target information: NPPB

Accession#:P16860 Gene ID:4879 NPPB NT-proBNP

Basic Description

Product Name	NT-proBNP Mouse mAb - Primary antibody, for ELISA,CLIA,GICA,Detection antibody,FIA various applications, from Human, generated in Mouse, specific to NT-proBNP, IgG1
Synonyms	Brain natriuretic factor prohormone \| Gamma-brain natriuretic peptide \| Iso-ANP \| preproBNP \| proBNP
Specifications & Purity	ExactAb™, Validated, Carrier Free, Azide Free, 8.66mg/mL
Host Species	Mouse
Host species	Mouse
Specificity	NT-proBNP
Application	ELISA,CLIA,GICA,Detection antibody,FIA
Immunogen	NT-proBNP antigen (His27-His134)
Epitope	27-31aa
Conjugation	Unconjugated
Grade	Azide Free, Carrier Free, ExactAb™, Validated
Product Description	Application Detection antibody ELISA: 1μg/ml； CLIA: 1μg/ml； GICA/FIA: 1mg/ml; Protein function Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.

Product Properties

Antibody Type	Primary antibody
Clonality	Monoclonal antibodies
Isotype	IgG1
SDS-PAGE	150kDa
Purification Method	Caprylic acid precipitation
Matched Pairs	[Ab008412] Recombinant NT-proBNP Antibody as Capture antibody in ELISA, CLIA
Source	Hybridoma supernatant
Form	Liquid
Concentration	8.66mg/mL
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at -20±5℃ for three years. Avoid freeze/thaw.

Images

NT-proBNP Mouse mAb (Ab007709)-Binding ELISA
The binding activity of NT-proBNP Mouse mAb with NT-proBNP antigen.

NT-proBNP Standard Curve with Antibody Pair (Ab008412 & Ab007709) in CLIA

· Capture antibody: Recombinant NT-proBNP Antibody (Ab008412) at 1.0 μg/mL.

· Detection antibody: NT-proBNP Mouse mAb (Ab007709) at 1.0 μg/mL.

· Linear Range: 5-35000 pg/mL, R ≥ 0.99.

NT-proBNP Standard Curve with Antibody Pair (Ab008412 & Ab007709) in FLFIA

· Capture antibody: Recombinant NT-proBNP Antibody (Ab008412) at 1.0 mg/mL.

· Detection antibody: NT-proBNP Mouse mAb (Ab007709) at 1.0 mg/mL.

· Linear Range: 0-35000 pg/mL, LLOD: 50 pg/mL.

Associated Targets(Human)

GCSAM Tbio Germinal center-associated signaling and motility protein (0 Activities)

Discover Target: GCSAM

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

NPPB Tbio Natriuretic peptides B (0 Activities)

Discover Target: NPPB

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Application

Dilution info

ELISA

1 μg/mL

CLIA

1 μg/mL

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number	Certificate Type	Date	Item
ZJ23F0600291	Certificate of Analysis	Jun 07, 2023	Ab007709
ZJ23F0600290	Certificate of Analysis	Jun 07, 2023	Ab007709

References

1. Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P et al.. (2004) The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).. Genome Res, 14 (10B): (2121-7). [PMID:15489334] [10.1021/op500134e]
2. Koller, K J KJ and 6 more authors.. (1991) Selective activation of the B natriuretic peptide receptor by C-type natriuretic peptide (CNP).. Science (New York, N.Y.), (5): [PMID:1672777]
3. Kambayashi, Y Y and 8 more authors.. (1990) Isolation and sequence determination of human brain natriuretic peptide in human atrium.. FEBS letters, (1): [PMID:2136732]
4. Hino, J J, Tateyama, H H, Minamino, N N, Kangawa, K K and Matsuo, H H.. (1990) Isolation and identification of human brain natriuretic peptides in cardiac atrium.. Biochemical and biophysical research communications, (16): [PMID:2138890]
5. Sudoh, T T and 5 more authors.. (1989) Cloning and sequence analysis of cDNA encoding a precursor for human brain natriuretic peptide.. Biochemical and biophysical research communications, (31): [PMID:2522777]
6. Seilhamer, J J JJ and 6 more authors.. (1989) Human and canine gene homologs of porcine brain natriuretic peptide.. Biochemical and biophysical research communications, (15): [PMID:2597152]
7. Jensen, K T KT, Carstens, J J and Pedersen, E B EB.. (1998) Effect of BNP on renal hemodynamics, tubular function and vasoactive hormones in humans.. The American journal of physiology, [PMID:9458824]
8. Yan, W W, Wu, F F, Morser, J J and Wu, Q Q.. (2000) Corin, a transmembrane cardiac serine protease, acts as a pro-atrial natriuretic peptide-converting enzyme.. Proceedings of the National Academy of Sciences of the United States of America, (18): [PMID:10880574]
9. Ohta, Y Y and 12 more authors.. (2000) Carvedilol enhances atrial and brain natriuretic peptide mRNA expression and release in rat heart.. Journal of cardiovascular pharmacology, [PMID:11206715]
10. Richards, A M AM and 9 more authors.. (2001) Plasma N-terminal pro-brain natriuretic peptide and adrenomedullin: prognostic utility and prediction of benefit from carvedilol in chronic ischemic left ventricular dysfunction. Australia-New Zealand Heart Failure Group.. Journal of the American College of Cardiology, (1): [PMID:11401111]
11. Frantz, Robert P RP and 11 more authors.. (2005) Carvedilol therapy is associated with a sustained decline in brain natriuretic peptide levels in patients with congestive heart failure.. American heart journal, [PMID:15864245]
12. Gregory, S G SG and 178 more authors.. (2006) The DNA sequence and biological annotation of human chromosome 1.. Nature, (18): [PMID:16710414]
13. He, Xiao-lin XL, Dukkipati, Abhiram A and Garcia, K Christopher KC.. (2006) Structural determinants of natriuretic peptide receptor specificity and degeneracy.. Journal of molecular biology, (25): [PMID:16870210]
14. Lam, Carolyn S P CS, Burnett, John C JC, Costello-Boerrigter, Lisa L, Rodeheffer, Richard J RJ and Redfield, Margaret M MM.. (2007) Alternate circulating pro-B-type natriuretic peptide and B-type natriuretic peptide forms in the general population.. Journal of the American College of Cardiology, (20): [PMID:17367664]
15. Ralat, Luis A LA and 6 more authors.. (2011) Insulin-degrading enzyme modulates the natriuretic peptide-mediated signaling response.. The Journal of biological chemistry, (11): [PMID:21098034]
16. Keane, Fiona M FM, Nadvi, Naveed A NA, Yao, Tsun-Wen TW and Gorrell, Mark D MD.. (2011) Neuropeptide Y, B-type natriuretic peptide, substance P and peptide YY are novel substrates of fibroblast activation protein-α.. The FEBS journal, [PMID:21314817]
17. Peng, Jianhao J and 5 more authors.. (2011) Glycosylation and processing of pro-B-type natriuretic peptide in cardiomyocytes.. Biochemical and biophysical research communications, (5): [PMID:21763278]
18. Huntley, Brenda K and 5 more authors.. (2015) Pro-B-type natriuretic peptide-1-108 processing and degradation in human heart failure.. Circulation. Heart failure, [PMID:25339504]

Solution Calculators

Molarity Calculator

Determine the necessary mass, volume, or concentration for preparing a solution.

Dilution Calculator

Determine the dilution needed to prepare a stock solution.