Basic Description

Product Name	NUR77 Antibody - Primary antibody, specific to NUR77, Rabbit IgG
Synonyms	Early response protein NAK1 antibody \| GFRP 1 antibody \| GFRP antibody \| GFRP1 antibody \| Growth factor inducible nuclear protein N10 antibody \| Growth Factor Inducible Nuclear Protein NP10 antibody \| Growth Factor Response Protein 1 antibody \| Hbr1 antib
Specifications & Purity	ExactAb™, Validated, 0.3 mg/mL
Host species	Rabbit
Specificity	NUR77
Immunogen	A synthetic peptide derived from human NUR77 (AA 16-29)
Positive Control	WB: Hep G2, A431 and HeLa cell lysates
Conjugation	Unconjugated
Grade	ExactAb™, Validated
Product Description	Rabbit anti Human NUR77 Antibody, Polyclonal, could be used for WB and so on. Application WB: 1/500-1/1000 Protein Function Orphan nuclear receptor. May act concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Binds the NGFI-B response element (NBRE) 5'-AAAAGGTCA-3' (By similarity). May inhibit NF-kappa-B transactivation of IL2.

Product Properties

Isotype	Rabbit IgG
SDS-PAGE	150 kDa
Purification Method	Protein A purified
Form	Liquid
Concentration	0.3 mg/mL
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

NUR77 Antibody (Ab119062) - Western Blot
All lanes: NUR77 Antibody (Ab119062) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 64 kDa
Observed band size: 38, 43, 55-80 kDa. The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID: 30224829, 28111880, 29669342).
Exposure time: 100.0 s

Associated Targets(Human)

NR4A1 Tchem Nuclear receptor subfamily 4 group A member 1 (0 Activities)

Discover Target: NR4A1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	References

Application

Application	Dilution info
WB	1/500-1/1000 "> 1/500-1/1000

Certificates

Certificate of Analysis(COA)

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2 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0404685	Certificate of Analysis	Apr 19, 2024	Ab119062
ZJ24F0404684	Certificate of Analysis	Apr 19, 2024	Ab119062

FAQs and Articles

Citations of This Product

How to Cite Aladdin Scientific Products?

1. Li XS, Ren YC, Bao YZ, Liu J, Zhang XK, Zhang YW, Sun XL, Yao XS, Tang JS.. (2018) Synthesis of C3-Neoglycosides of digoxigenin and their anticancer activities.. Eur J Med Chem, 145 (252-262). [PMID:29329000] [10.1016/j.ejmech.2017.12.086]

References

1. Bondy GP. (1991) Phorbol ester, forskolin, and serum induction of a human colon nuclear hormone receptor gene related to the NUR 77/NGFI-B genes.. Cell Growth Differ, 2 (4): (203-8). [PMID:1651101] [10.1021/op500134e]
2. Nakai A, Kartha S, Sakurai A, Toback FG, DeGroot LJ. (1990) A human early response gene homologous to murine nur77 and rat NGFI-B, and related to the nuclear receptor superfamily.. Mol Endocrinol, 4 (10): (1438-43). [PMID:2283997] [10.1021/op500134e]
3. Chang C, Kokontis J, Liao SS, Chang Y. (1989) Isolation and characterization of human TR3 receptor: a member of steroid receptor superfamily.. J Steroid Biochem, 34 (1-6): (391-5). [PMID:2626032] [10.1021/op500134e]
4. Park KC, Song KH, Chung HK, Kim H, Kim DW, Song JH, Hwang ES, Jung HS, Park SH, Bae I, Lee IK, Choi HS, Shong M. (2005) CR6-interacting factor 1 interacts with orphan nuclear receptor Nur77 and inhibits its transactivation.. Mol Endocrinol, 19 (1): (12-24). [PMID:15459248] [10.1021/op500134e]
5. Zhan Y, Du X, Chen H, Liu J, Zhao B, Huang D, Li G, Xu Q, Zhang M, Weimer BC et al.. (2008) Cytosporone B is an agonist for nuclear orphan receptor Nur77.. Nat Chem Biol, 4 (9): (548-56). [PMID:18690216] [10.1021/op500134e]
6. Solt LA, Wang Y, Banerjee S, Hughes T, Kojetin DJ, Lundasen T, Shin Y, Liu J, Cameron MD, Noel R et al.. (2012) Regulation of circadian behaviour and metabolism by synthetic REV-ERB agonists.. Nature, 485 (7396): (62-8). [PMID:22460951] [10.1021/op500134e]
7. Xia Z, Cao X, Rico-Bautista E, Yu J, Chen L, Chen J, Bobkov A, Wolf DA, Zhang XK, Dawson MI.. (2013) Relative impact of 3- and 5-hydroxyl groups of cytosporone B on cancer cell viability.. Medchemcomm, 4 (2): (332-339). [PMID:24795803] [10.1039/c2md20243c]
8. Chen F, Liu J, Huang M, Hu M, Su Y, Zhang XK.. (2014) Identification of a New RXRα Antagonist Targeting the Coregulator-Binding Site.. ACS Med Chem Lett, 5 (7): (736-741). [PMID:25057340] [10.1021/ml5000405]
9. Xu D, Cai L, Guo S, Xie L, Yin M, Chen Z, Zhou H, Su Y, Zeng Z, Zhang X.. (2017) Virtual screening and experimental validation identify novel modulators of nuclear receptor RXRα from Drugbank database.. Bioorg Med Chem Lett, 27 (4): (1055-1061). [PMID:28089347] [10.1016/j.bmcl.2016.12.058]
10. Wang DD, Li XS, Bao YZ, Liu J, Zhang XK, Yao XS, Sun XL, Tang JS.. (2017) Synthesis of MeON-neoglycosides of digoxigenin with 6-deoxy- and 2,6-dideoxy-d-glucose derivatives and their anticancer activity.. Bioorg Med Chem Lett, 27 (15): (3359-3364). [PMID:28633895] [10.1016/j.bmcl.2017.06.008]
11. Li XS, Ren YC, Bao YZ, Liu J, Zhang XK, Zhang YW, Sun XL, Yao XS, Tang JS.. (2018) Synthesis of C3-Neoglycosides of digoxigenin and their anticancer activities.. Eur J Med Chem, 145 (252-262). [PMID:29329000] [10.1016/j.ejmech.2017.12.086]
12. Li B,Yao J,Guo K,He F,Chen K,Lin Z,Liu S,Huang J,Wu Q,Fang M,Zeng J,Wu Z. (2020) Design, synthesis, and biological evaluation of 5-((8-methoxy-2-methylquinolin-4-yl)amino)-1H-indole-2-carbohydrazide derivatives as novel Nur77 modulators.. Eur J Med Chem, 204 (112608-112608). [PMID:32717483] [10.1016/j.ejmech.2020.112608]
13. Harant, Hanna H and Lindley, Ivan J D IJ.. (2004) Negative cross-talk between the human orphan nuclear receptor Nur77/NAK-1/TR3 and nuclear factor-kappaB.. Nucleic acids research, [PMID:15466594]
14. Cao, Xihua X and 8 more authors.. (2004) Retinoid X receptor regulates Nur77/TR3-dependent apoptosis [corrected] by modulating its nuclear export and mitochondrial targeting.. Molecular and cellular biology, [PMID:15509776]
15. Scherer, Steven E SE and 216 more authors.. (2006) The finished DNA sequence of human chromosome 12.. Nature, (16): [PMID:16541075]
16. Zhao, Wen-Xiu WX and 7 more authors.. (2007) Orphan receptor TR3 attenuates the p300-induced acetylation of retinoid X receptor-alpha.. Molecular endocrinology (Baltimore, Md.), [PMID:17761950]
17. Daub, Henrik H and 9 more authors.. (2008) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle.. Molecular cell, (8): [PMID:18691976]
18. Kang, Shin-Ae SA and 5 more authors.. (2010) Regulation of Nur77 protein turnover through acetylation and deacetylation induced by p300 and HDAC1.. Biochemical pharmacology, (15): [PMID:20438716]
19. Papac-Milicevic, Nikolina N and 13 more authors.. (2012) The interferon stimulated gene 12 inactivates vasculoprotective functions of NR4A nuclear receptors.. Circulation research, (13): [PMID:22427340]
20. Zhan, Yan-yan YY and 24 more authors.. (2012) The orphan nuclear receptor Nur77 regulates LKB1 localization and activates AMPK.. Nature chemical biology, [PMID:22983157]

Solution Calculators

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SKU	Size	Availability	Price
Ab119062-10μl	10μl	In stock	$69.90
Ab119062-50μl	50μl	In stock	$209.90
Ab119062-100μl	100μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$329.90
Ab119062-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$2,399.90

NUR77 Antibody - Primary antibody, specific to NUR77, Rabbit IgG