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o-Phenylenediamine - 99.5%, high purity , CAS No.95-54-5

461 Citations
  • ≥99.5%
Item Number
P107882
Grouped product items
SKUSizeAvailabilityPrice Qty
P107882-25g
25g
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$25.90
P107882-100g
100g
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$59.90
P107882-500g
500g
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$199.90
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Peroxidase substrate

Basic Description

SynonymsFT-0662537 | NCGC00090885-03 | o-Phenylene diamine | O-PHENYLENEDIAMINE [HSDB] | TIABENDAZOLE IMPURITY A [EP IMPURITY] | DTXCID905881 | NCI60_004318 | NSC 5354 | BCP25295 | 1,2-Benzenediamine | 1,2-benzenedi-amine | NCGC00090885-02 | o-Fenylendiamin [Czec
Specifications & Purity≥99.5%
Storage TempStore at 2-8°C,Protected from light,Argon charged
Shipped InWet ice
Product Description

A peroxidase substrate that is widely used to detect horseradish peroxidase activity in ELISA and solution assays. Produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm.
Used to detect horseradish peroxidase activity

Associated Targets(Human)

AR Tclin Androgen Receptor (11781 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
AHR Tclin Aryl hydrocarbon receptor (1071 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
CASP1 Tchem Caspase-1 (6235 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
ALOX15 Tchem Arachidonate 15-lipoxygenase (7108 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
CASP7 Tchem Caspase-7 (3146 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
CYP3A4 Tclin Cytochrome P450 3A4 (53859 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
A498 (42825 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
ACHN (49357 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
CAKI-1 (44928 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
CCRF-CEM (65223 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
COLO 205 (50209 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
DLD-1 (17511 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
DMS-273 (14108 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
HL-60 (67320 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
HT-29 (80576 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
K562 (73714 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
KM12 (47707 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
M14 (47487 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
MOLT-4 (49676 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
OVCAR-3 (48710 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
OVCAR-4 (44535 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
OVCAR-5 (45555 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
OVCAR-8 (47708 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
RPMI-8226 (44974 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
RXF 393 (41971 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
SF-295 (48000 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
SK-MEL-2 (46422 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
SK-MEL-28 (48833 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
SK-MEL-5 (47095 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
SK-OV-3 (52876 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
SN12C (47755 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
SNB-19 (46794 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
TK-10 (45540 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
U-251 (51189 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
UACC-62 (47335 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
UO-31 (46270 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
786-0 (47912 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
A549 (127892 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
NCI-H322M (45589 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
HCC 2998 (41480 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
HCT-116 (91556 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
HOP-18 (11577 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
HOP-92 (41141 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
NCI-H460 (60772 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
SF-268 (49410 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
IGROV-1 (47897 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
LOX IMVI (44321 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
HOP-62 (47048 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
LXFL 529 (14112 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
Lymphoblastoid cell (5959 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID

Associated Targets(non-human)

Mmp9 Matrix metalloproteinase 9 (38 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
Pseudomonas aeruginosa (123386 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
Staphylococcus aureus (210822 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
Escherichia coli (133304 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
Serratia marcescens (3237 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
Bacillus subtilis (32866 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
Luciferin 4-monooxygenase (66902 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
ARG1 Arginase-1 (70 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
Nfe2l2 Nuclear factor erythroid 2-related factor 2 (214 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
Rorc Nuclear receptor ROR-gamma (89407 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
Nr1i2 Nuclear receptor subfamily 1 group I member 2 (14 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID

Mechanisms of Action

Mechanism of ActionAction Typetarget IDTarget NameTarget TypeTarget OrganismBinding Site NameReferences

Names and Identifiers

IUPAC Name benzene-1,2-diamine
INCHI InChI=1S/C6H8N2/c7-5-3-1-2-4-6(5)8/h1-4H,7-8H2
InChi Key GEYOCULIXLDCMW-UHFFFAOYSA-N
Canonical SMILES C1=CC=C(C(=C1)N)N
Isomeric SMILES C1=CC=C(C(=C1)N)N
WGK Germany 3
RTECS SS7875000
PubChem CID 7243
UN Number 1673
Packing Group III
Molecular Weight 108.14
Beilstein 606074
Reaxy-Rn 606074

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

26 results found

Lot NumberCertificate TypeDateItem
K2321500Certificate of AnalysisSep 18, 2024 P107882
K2321497Certificate of AnalysisSep 18, 2024 P107882
H2401190Certificate of AnalysisJul 12, 2024 P107882
H2401193Certificate of AnalysisJul 12, 2024 P107882
H2401191Certificate of AnalysisJul 12, 2024 P107882
I2301580Certificate of AnalysisJun 19, 2024 P107882
I2301579Certificate of AnalysisJun 19, 2024 P107882
F2327910Certificate of AnalysisApr 03, 2024 P107882
D2409238Certificate of AnalysisMar 22, 2024 P107882
D2409239Certificate of AnalysisMar 22, 2024 P107882
I2301581Certificate of AnalysisAug 18, 2023 P107882
K2211417Certificate of AnalysisAug 16, 2023 P107882
A2218089Certificate of AnalysisAug 10, 2023 P107882
A2218090Certificate of AnalysisAug 10, 2023 P107882
F2327922Certificate of AnalysisJun 15, 2023 P107882
F2327920Certificate of AnalysisJun 15, 2023 P107882
F2327912Certificate of AnalysisJun 15, 2023 P107882
I2202770Certificate of AnalysisJun 13, 2023 P107882
I2202771Certificate of AnalysisJun 13, 2023 P107882
I2202711Certificate of AnalysisJun 13, 2023 P107882
D2307413Certificate of AnalysisJul 29, 2022 P107882
I2103143Certificate of AnalysisJul 14, 2022 P107882
H2011124Certificate of AnalysisMay 10, 2022 P107882
D2109327Certificate of AnalysisFeb 15, 2022 P107882
A2218091Certificate of AnalysisDec 09, 2021 P107882
A2218088Certificate of AnalysisDec 09, 2021 P107882

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Chemical and Physical Properties

SolubilitySoluble in alcohol, chloroform, ether, slightly soluble in water. Soluble in hot water.
SensitivityLight sensitive. air sensitive
Flash Point(°F)276.8 °F
Flash Point(°C)136°C
Boil Point(°C)256～258°C
Melt Point(°C)103-104°C

Safety and Hazards(GHS)

Pictogram(s) GHS06,   GHS08,   GHS09
Signal Danger
Hazard Statements

H319:Causes serious eye irritation

H301:Toxic if swallowed

H341:Suspected of causing genetic defects

H351:Suspected of causing cancer

H317:May cause an allergic skin reaction

H410:Very toxic to aquatic life with long lasting effects

H312+H332:Harmful in contact with skin or if inhaled

Precautionary Statements

P261:Avoid breathing dust/fume/gas/mist/vapors/spray.

P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do - continue rinsing.

P273:Avoid release to the environment.

P280:Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352:IF ON SKIN: wash with plenty of water.

P321:Specific treatment (see ... on this label).

P405:Store locked up.

P501:Dispose of contents/container to ...

P264:Wash hands [and …] thoroughly after handling.

P281:Use personal protective equipment as required.

P271:Use only outdoors or in a well-ventilated area.

P270:Do not eat, drink or smoke when using this product.

P304+P340:IF INHALED: Remove person to fresh air and keep comfortable for breathing.

P272:Contaminated work clothing should not be allowed out of the workplace.

P333+P313:IF SKIN irritation or rash occurs: Get medical advice/attention.

P362+P364:Take off contaminated clothing and wash it before reuse.

P391:Collect spillage.

P330:Rinse mouth.

P203:Obtain, read and follow all safety instructions before use.

P264+P265:Wash hands [and …] thoroughly after handling. Do not touch eyes.

P301+P316:IF SWALLOWED: Get emergency medical help immediately.

P318:if exposed or concerned, get medical advice.

P317:Get emergency medical help.

P337+P317:If eye irritation persists: Get medical help.

WGK Germany 3
RTECS SS7875000
Reaxy-Rn 606074
Class 6.1
Merck Index 7284

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152. Xuewei Tu, Luping Zhang, Yutong Chen, Shouxin Zhu, Can Sun, Jiali Jin, Min Liu, Hui Zheng.  (2023)  The efficient CO2 fixation catalyzed by K-doped g-C3N4 catalyst for synthesizing benzimidazoles at atmospheric pressure.  Greenhouse Gases-Science and Technology,  13  (5): (689-698).  [PMID:] [10.1002/ghg.2222]
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156. Bin Liu, Ziying Wang, Tao Wei, Zongming Liu, Jinkai Li.  (2023)  Bimetallic FeMn-N nanoparticles as nanocatalyst with dual enzyme-mimic activities for simultaneous colorimetric detection and degradation of hydroquinone.  Journal of Environmental Chemical Engineering,  11  (110186).  [PMID:] [10.1016/j.jece.2023.110186]
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158. Guo Jingfang, Wang Guodong, Zou Jing, Lei Zhen.  (2023)  DNA controllable peroxidase-like activity of Ti3C2 nanosheets for colorimetric detection of microcystin-LR.  ANALYTICAL AND BIOANALYTICAL CHEMISTRY,  415  (17): (3559-3569).  [PMID:37198360] [10.1007/s00216-023-04745-0]
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164. Zhang Zhe, Xu Yongzhi, Zhu Tingting, Sang Zhiqin, Guo Xiaoli, Sun Yu, Hao Yuanping, Wang Wanchun.  (2023)  Hypoxia mitigation by manganese-doped carbon dots for synergistic photodynamic therapy of oral squamous cell carcinoma.  Frontiers in Bioengineering and Biotechnology,  11    [PMID:37152644] [10.3389/fbioe.2023.1153196]
165. Cheng Lu, Wu Fengxia, Tian Yu, Lv Xiali, Li Fenghua, Xu Guobao, Hsu Hsien-Yi, Zhang Yongjun, Niu Wenxin.  (2023)  Gap engineering of sandwich plasmonic gap nanostructures for boosting plasmon-enhanced electrocatalysis.  Nano Research,  16  (7): (8961-8969).  [PMID:] [10.1007/s12274-023-5620-3]
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177. Wei Jiang, Xiaoyuan Wang, YunJiao Ma, Ming Du, Chao Wu, Xianbing Xu.  (2023)  Mechanism of Carbon Skeleton Formation of 2,3,5-Trimethylpyrazine via a Conversion Reaction between Methylglyoxal and Glyoxal.  JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,  71  (13): (5337–5344).  [PMID:36942552] [10.1021/acs.jafc.2c08745]
178. Qingcheng Song, Yiran Zhang, Xiangtian Deng, Haiyue Zhao, Yueyao Zhang, Junyong Li, Wei Chen, Hongzhi Hu, Yingze Zhang.  (2023)  Amplification of oxidative stress with a hyperthermia-enhanced chemodynamic process and MTH1 inhibition for sequential tumor nanocatalytic therapy.  Journal of Materials Chemistry B,  11  (17): (3836-3850).  [PMID:36976579] [10.1039/D2TB02673B]
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183. Ding Minling, Niu Huizhe, Guan Ping, Hu Xiaoling.  (2023)  Molecularly imprinted sensor based on poly-o-phenylenediamine-hydroquinone polymer for β-amyloid-42 detection.  ANALYTICAL AND BIOANALYTICAL CHEMISTRY,  415  (8): (1545-1557).  [PMID:36808273] [10.1007/s00216-023-04552-7]
184. Yu Yao, Li Yansong, Xu Ning, Zha Yonghong, Bai Xue, Liu Mingyuan, Zhou Yu, Liu Xiaolei.  (2023)  Fluorescence ratio immunoassay for fumonisin B1 based on the oxidase characteristics of the growth of monodispersed 2-D MnO2 nanosheet on an individual gold nanoparticle (AuNP@MnO2).  MICROCHIMICA ACTA,  190  (3): (1-8).  [PMID:36790594] [10.1007/s00604-023-05675-5]
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