| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab170578-100μg | 100μg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $109.90 | |
| Ab170578-1mg | 1mg | In stock | $507.90 | |
| Ab170578-5mg | 5mg | In stock | $1,257.90 | |
| Ab170578-10mg | 10mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $1,857.90 |
Purity>95% (SDS-PAGE&SEC); Endotoxin Level<1.0EU/mg; Human IgG1; CHO; ELISA, FACS, Functional assay, Animal Model; Unconjugated
| Product Name | Oleclumab (anti-CD73) - Primary antibody, for ELISA,Functional Assay,Flow cytometry,Kinetics (BLI),Kinetics (SPR) various applications, specific to NT5E, >95%, high purity, Human IgG1 |
|---|---|
| Synonyms | 5'-NT | EC 3.1.3.5 | Ecto-5'-nucleotidase |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Low Endotoxin, Azide Free, Recombinant, ≥95%(SDS-PAGE&SEC), Lot by Lot |
| Host species | Human |
| Specificity | NT5E |
| Application | ELISA,Functional Assay,Flow cytometry,Kinetics (BLI),Kinetics (SPR) |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, Low Endotoxin, Recombinant, Validated |
| Action Type | INHIBITOR |
| Mechanism of action | Antibody of Ecto-5'-Nucleotidase |
| Product Description | Oleclumab (anti-NT5E) is a human IgG1λ anti-CD73 monoclonal antibody that inhibits CD73 function. Oleclumab has an anti-tumor activity. Purity |
| Antibody Type | Primary antibody |
|---|---|
| Clonality | Recombinant |
| Isotype | Human IgG1 |
| Light Chain Type | lambda |
| SDS-PAGE | 28.1 kDa (Light Chain) & 50.0 kDa (Heavy Chain), under reducing conditions; 172.2 kDa, under non-reducing conditions. |
| Purification Method | Protein A purified |
| Purity | >95% |
| Source | CHO supernatant |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at -80°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at -80℃ for 24 months. Upon delivery aliquot. Avoid freeze/thaw cycle. |
| CAS | 1803176-05-7 |
Oleclumab (anti-CD73) (Ab170578) - Flow Cytometry
Flow Cytometry analysis of A431 cells labelling CD73 (red) with Oleclumab (anti-CD73) (Ab170578). Goat Anti-Human IgG (PE) (Ab175838) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, human IgG (Ab170213). Black - Unlabelled control, cells without incubation with primary antibody.
Oleclumab (anti-CD73) (Ab170578) - SEC
The purity of Oleclumab (anti-CD73) (Ab170578) is more than 95% verified by HPLC.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| IMGT/mAb-DB | 729 |
|---|
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F1201923 | Certificate of Analysis | Dec 06, 2023 | Ab170578 |
| ZJ23F1201924 | Certificate of Analysis | Dec 06, 2023 | Ab170578 |
| 1. Geoghegan JC, Diedrich G, Lu X, Rosenthal K, Sachsenmeier KF, Wu H, Dall'Acqua WF, Damschroder MM. (2016) Inhibition of CD73 AMP hydrolysis by a therapeutic antibody with a dual, non-competitive mechanism of action.. MAbs, 8 (3): (454-67). [PMID:26854859] |
| 2. Hay CM, Sult E, Huang Q, Mulgrew K, Fuhrmann SR, McGlinchey KA, Hammond SA, Rothstein R, Rios-Doria J, Poon E et al.. (2016) Targeting CD73 in the tumor microenvironment with MEDI9447.. Oncoimmunology, 5 (8): (e1208875). [PMID:27622077] |
| 3. Eliahu S, Lecka J, Reiser G, Haas M, Bigonnesse F, Lévesque SA, Pelletier J, Sévigny J, Fischer B.. (2010) Diadenosine 5',5''-(boranated)polyphosphonate analogues as selective nucleotide pyrophosphatase/phosphodiesterase inhibitors.. J Med Chem, 53 (24): (8485-8497). [PMID:21090681] |
| 4. al-Rashida M, Raza R, Abbas G, Shah MS, Kostakis GE, Lecka J, Sévigny J, Muddassar M, Papatriantafyllopoulou C, Iqbal J.. (2013) Identification of novel chromone based sulfonamides as highly potent and selective inhibitors of alkaline phosphatases.. Eur J Med Chem, 66 (438-449). [PMID:23831808] |
| 5. Gillerman I, Lecka J, Simhaev L, Munkonda MN, Fausther M, Martín-Satué M, Senderowitz H, Sévigny J, Fischer B.. (2014) 2-Hexylthio-β,γ-CH2-ATP is an effective and selective NTPDase2 inhibitor.. J Med Chem, 57 (14): (5919-5934). [PMID:24972256] |
| 6. Lee SY, Perotti A, De Jonghe S, Herdewijn P, Hanck T, Müller CE.. (2016) Thiazolo[3,2-a]benzimidazol-3(2H)-one derivatives: Structure-activity relationships of selective nucleotide pyrophosphatase/phosphodiesterase1 (NPP1) inhibitors.. Bioorg Med Chem, 24 (14): (3157-3165). [PMID:27265686] |
| 7. Abdel-Magid AF.. (2017) Inhibitors of CD73 May Provide a Treatment for Cancer and Autoimmune Diseases.. ACS Med Chem Lett, 8 (8): (781-782). [PMID:28835785] |
| 8. Junker A, Renn C, Dobelmann C, Namasivayam V, Jain S, Losenkova K, Irjala H, Duca S, Balasubramanian R, Chakraborty S, Börgel F, Zimmermann H, Yegutkin GG, Müller CE, Jacobson KA.. (2019) Structure-Activity Relationship of Purine and Pyrimidine Nucleotides as Ecto-5'-Nucleotidase (CD73) Inhibitors.. J Med Chem, 62 (7): (3677-3695). [PMID:30895781] |
| 9. Du X,Moore J,Blank BR,Eksterowicz J,Sutimantanapi D,Yuen N,Metzger T,Chan B,Huang T,Chen X,Chen Y,Duong F,Kong W,Chang JH,Sun J,Zavorotinskaya T,Ye Q,Junttila MR,Ndubaku C,Friedman LS,Fantin VR,Sun D. (2020) Orally Bioavailable Small-Molecule CD73 Inhibitor (OP-5244) Reverses Immunosuppression through Blockade of Adenosine Production.. J Med Chem, 63 (18): (10433-10459). [PMID:32865411] |
| 10. Misumi, Y Y, Ogata, S S, Ohkubo, K K, Hirose, S S and Ikehara, Y Y.. (1990) Primary structure of human placental 5'-nucleotidase and identification of the glycolipid anchor in the mature form.. European journal of biochemistry, (17): [PMID:2129526] |
| 11. Klemens, M R MR and 5 more authors.. (1990) Characterization of soluble vs membrane-bound human placental 5'-nucleotidase.. Biochemical and biophysical research communications, (15): [PMID:2173922] |
| 12. Hansen, K R KR, Resta, R R, Webb, C F CF and Thompson, L F LF.. (1995) Isolation and characterization of the promoter of the human 5'-nucleotidase (CD73)-encoding gene.. Gene, (29): [PMID:8566797] |
| 13. Otsuki, Tetsuji T and 24 more authors.. (2005) Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries.. DNA research : an international journal for rapid publication of reports on genes and genomes, [PMID:16303743] |
| 14. Ustunsoy, Hasim H and 5 more authors.. () The effects of pentoxifylline on the myocardial inflammation and ischemia-reperfusion injury during cardiopulmonary bypass.. Journal of cardiac surgery, [PMID:16426349] |
| 15. Wollscheid, Bernd B and 7 more authors.. (2009) Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins.. Nature biotechnology, [PMID:19349973] |
| 16. St Hilaire, Cynthia C and 20 more authors.. (2011) NT5E mutations and arterial calcifications.. The New England journal of medicine, (3): [PMID:21288095] |
| 17. Garavaglia, Silvia S and 9 more authors.. (2012) The high-resolution crystal structure of periplasmic Haemophilus influenzae NAD nucleotidase reveals a novel enzymatic function of human CD73 related to NAD metabolism.. The Biochemical journal, (1): [PMID:21933152] |
| 18. Heuts, Dominic P H M DP and 6 more authors.. (2012) Crystal structure of a soluble form of human CD73 with ecto-5'-nucleotidase activity.. Chembiochem : a European journal of chemical biology, (5): [PMID:22997138] |
| 19. Knapp, Karen K and 5 more authors.. (2012) Crystal structure of the human ecto-5'-nucleotidase (CD73): insights into the regulation of purinergic signaling.. Structure (London, England : 1993), (5): [PMID:23142347] |
| 20. Fausther, Michel M, Lavoie, Elise G EG, Goree, Jessica R JR, Baldini, Giulia G and Dranoff, Jonathan A JA.. (2014) NT5E mutations that cause human disease are associated with intracellular mistrafficking of NT5E protein.. PloS one, [PMID:24887587] |
| 21. Channar, Pervaiz Ali PA and 8 more authors.. (2017) Isonicotinohydrazones as inhibitors of alkaline phosphatase and ecto-5'-nucleotidase.. Chemical biology & drug design, [PMID:27589035] |
