pAb; Rabbit anti Human Osteopontin Antibody; WB, IHC; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab119430-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $66.90 | |
| Ab119430-20μl | 20μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $89.90 | |
| Ab119430-50μl | 50μl | In stock | $189.90 | |
| Ab119430-100μl | 100μl | In stock | $309.90 | |
| Ab119430-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,299.90 |
pAb; Rabbit anti Human Osteopontin Antibody; WB, IHC; Unconjugated
| Product Name | Osteopontin Antibody - Primary antibody, specific to Osteopontin, Rabbit IgG |
|---|---|
| Synonyms | BNSP antibody; Bone sialoprotein 1 antibody; Bone sialoprotein I antibody; BSP I antibody; BSPI antibody; Early T lymphocyte activation 1 antibody; ETA 1 antibody; ETA1 antibody; MGC110940 antibody; Nephropontin antibody; OPN antibody; Osteopontin antibod |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, High performance, Lot by Lot |
| Host species | Rabbit |
| Specificity | Osteopontin |
| Immunogen | Recombinant Osteopontin expressed in E.coli (AA 17-287). |
| Positive Control | WB: A549, Hep G2, HEK293, RAW 264.7 and NIH/3T3 cell lysates. IHC: Human kidney tissue. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, High Performance, Validated |
| Product Description | Rabbit anti Human Osteopontin Antibody, Polyclonal, could be used for WB, IHC and so on. Application WB: 0.01-3 µg/mL IHC: 5-30 µg/mL Protein Function Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified + Protein A purified |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Osteopontin Antibody (Ab119430) - Western Blot
All lanes: Osteopontin Antibody (Ab119430) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 35 kDa
Observed band size: 32, 45 kDa
Exposure time: 15.6 s
Osteopontin Antibody (Ab119430) - IHC
DAB staining on IHC
Samples: Human Kidney Tissue
Primary Ab: 10 µg/mL Osteopontin Antibody (Ab119430)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Application | Dilution info |
|---|---|
| IHC | 5-30 µg/mL |
| WB | 0.01-3 µg/mL |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F1101832 | Certificate of Analysis | Nov 27, 2023 | Ab119430 |
| ZJ23F1101814 | Certificate of Analysis | Nov 27, 2023 | Ab119430 |
| 1. Kohri, K K and 9 more authors.. (1992) Molecular cloning and sequencing of cDNA encoding urinary stone protein, which is identical to osteopontin.. Biochemical and biophysical research communications, (30): [PMID:1575754] |
| 2. Shiraga, H H and 9 more authors.. (1992) Inhibition of calcium oxalate crystal growth in vitro by uropontin: another member of the aspartic acid-rich protein superfamily.. Proceedings of the National Academy of Sciences of the United States of America, (1): [PMID:1729712] |
| 3. Young, M F MF and 6 more authors.. (1990) cDNA cloning, mRNA distribution and heterogeneity, chromosomal location, and RFLP analysis of human osteopontin (OPN).. Genomics, [PMID:1974876] |
| 4. Kiefer, M C MC, Bauer, D M DM and Barr, P J PJ.. (1989) The cDNA and derived amino acid sequence for human osteopontin.. Nucleic acids research, (25): [PMID:2726470] |
| 5. Senger, D R DR, Perruzzi, C A CA, Papadopoulos, A A and Tenen, D G DG.. (1989) Purification of a human milk protein closely similar to tumor-secreted phosphoproteins and osteopontin.. Biochimica et biophysica acta, (13): [PMID:2736258] |
| 6. Crosby, A H AH, Edwards, S J SJ, Murray, J C JC and Dixon, M J MJ.. (1995) Genomic organization of the human osteopontin gene: exclusion of the locus from a causative role in the pathogenesis of dentinogenesis imperfecta type II.. Genomics, (1): [PMID:7665163] |
| 7. Saitoh, Y Y, Kuratsu, J J, Takeshima, H H, Yamamoto, S S and Ushio, Y Y.. (1995) Expression of osteopontin in human glioma. Its correlation with the malignancy.. Laboratory investigation; a journal of technical methods and pathology, [PMID:7837791] |
| 8. Hijiya, N N and 5 more authors.. (1994) Cloning and characterization of the human osteopontin gene and its promoter.. The Biochemical journal, (1): [PMID:7945249] |
| 9. Hillier, Ladeana W LW and 121 more authors.. (2005) Generation and annotation of the DNA sequences of human chromosomes 2 and 4.. Nature, (7): [PMID:15815621] |
| 10. Christensen, Brian B, Nielsen, Mette S MS, Haselmann, Kim F KF, Petersen, Torben E TE and Sørensen, Esben S ES.. (2005) Post-translationally modified residues of native human osteopontin are located in clusters: identification of 36 phosphorylation and five O-glycosylation sites and their biological implications.. The Biochemical journal, (15): [PMID:15869464] |
| 11. Otsuki, Tetsuji T and 24 more authors.. (2005) Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries.. DNA research : an international journal for rapid publication of reports on genes and genomes, [PMID:16303743] |
| 12. Beranova-Giorgianni, Sarka S, Zhao, Yingxin Y, Desiderio, Dominic M DM and Giorgianni, Francesco F.. (2006) Phosphoproteomic analysis of the human pituitary.. Pituitary, [PMID:16807684] |
| 13. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692] |
| 14. Tagliabracci, Vincent S VS and 8 more authors.. (2012) Secreted kinase phosphorylates extracellular proteins that regulate biomineralization.. Science (New York, N.Y.), (1): [PMID:22582013] |
| 15. Tagliabracci, Vincent S VS and 14 more authors.. (2015) A Single Kinase Generates the Majority of the Secreted Phosphoproteome.. Cell, (18): [PMID:26091039] |
