| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab119753-10μl | 10μl | In stock | $69.90 | |
| Ab119753-50μl | 50μl | In stock | $189.90 | |
| Ab119753-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $289.90 | |
| Ab119753-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,829.90 |
pAb; Rabbit anti Human p46 Antibody; WB, IHC; Unconjugated
| Product Name | p46 Antibody - Primary antibody, specific to POLDIP3, Rabbit IgG |
|---|---|
| Synonyms | 46 kDa DNA polymerase delta interaction protein | p46 | S6K1 Aly/REF-like target | SKAR | 6 kDa DNA polymerase delta interaction protein antibody | DNA polymerase delta interacting protein 3 antibody | KIAA1649 antibody | P46 antibody | PDIP3_HUMAN antibo |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, 0.6 mg/mL |
| Host species | Rabbit |
| Specificity | POLDIP3 |
| Immunogen | Fusion protein of human POLDIP3 (AA 36-229) |
| Positive Control | WB: HeLa, Hep G2, Jurkat, HEK293, RAW 264.7 and C6 cell lysates. IHC: Human thyroid cancer tissue. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description | Rabbit anti Human p46 Antibody, Polyclonal, could be used for WB, IHC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 0.6 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
p46 Antibody (Ab119753) - Western Blot
All lanes: p46 Antibody (Ab119753) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 46 kDa
Observed band size: 44, 47 kDa
Exposure time: 55.2 s
p46 Antibody (Ab119753) - Western Blot
All lanes: p46 Antibody (Ab119753) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 46 kDa
Observed band size: 44, 47 kDa
Exposure time: 73.3 s
p46 Antibody (Ab119753) - IHC
Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using p46 Antibody (Ab119753). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000"> 1/500-1/1000 |
| IHC | 1/50 - 1/100 "> 1/50 - 1/100 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0404014 | Certificate of Analysis | Apr 01, 2024 | Ab119753 |
| ZJ24F0404013 | Certificate of Analysis | Apr 01, 2024 | Ab119753 |
| 1. Dunham, I I and 24 more authors.. (1999) The DNA sequence of human chromosome 22.. Nature, (2): [PMID:10591208] |
| 2. Hirosawa, M M, Nagase, T T, Murahashi, Y Y, Kikuno, R R and Ohara, O O.. (2001) Identification of novel transcribed sequences on human chromosome 22 by expressed sequence tag mapping.. DNA research : an international journal for rapid publication of reports on genes and genomes, (28): [PMID:11258795] |
| 3. Liu, Li L, Rodriguez-Belmonte, Esther M EM, Mazloum, Nayef N, Xie, Bin B and Lee, Marietta Y W T MY.. (2003) Identification of a novel protein, PDIP38, that interacts with the p50 subunit of DNA polymerase delta and proliferating cell nuclear antigen.. The Journal of biological chemistry, (21): [PMID:12522211] |
| 4. Collins, John E JE and 8 more authors.. (2003) Reevaluating human gene annotation: a second-generation analysis of chromosome 22.. Genome research, [PMID:12529303] |
| 5. Richardson, Celeste J CJ and 6 more authors.. (2004) SKAR is a specific target of S6 kinase 1 in cell growth control.. Current biology : CB, (7): [PMID:15341740] |
| 6. Collins, John E JE and 12 more authors.. (2004) A genome annotation-driven approach to cloning the human ORFeome.. Genome biology, [PMID:15461802] |
| 7. Smyk, Amelia A, Szuminska, Magdalena M, Uniewicz, Katarzyna A KA, Graves, Lee M LM and Kozlowski, Piotr P.. (2006) Human enhancer of rudimentary is a molecular partner of PDIP46/SKAR, a protein interacting with DNA polymerase delta and S6K1 and regulating cell growth.. The FEBS journal, [PMID:16984396] |
| 8. Matsuoka, Shuhei S and 12 more authors.. (2007) ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage.. Science (New York, N.Y.), (25): [PMID:17525332] |
| 9. Yu, Li-Rong LR and 5 more authors.. (2007) Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra.. Journal of proteome research, [PMID:17924679] |
| 10. Ma, Xiaoju Max XM, Yoon, Sang-Oh SO, Richardson, Celeste J CJ, Jülich, Kristina K and Blenis, John J.. (2008) SKAR links pre-mRNA splicing to mTOR/S6K1-mediated enhanced translation efficiency of spliced mRNAs.. Cell, (18): [PMID:18423201] |
| 11. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332] |
| 12. Dufu, Kobina K and 5 more authors.. (2010) ATP is required for interactions between UAP56 and two conserved mRNA export proteins, Aly and CIP29, to assemble the TREX complex.. Genes & development, (15): [PMID:20844015] |
| 13. Bienvenut, Willy V WV and 6 more authors.. (2012) Comparative large scale characterization of plant versus mammal proteins reveals similar and idiosyncratic N-α-acetylation features.. Molecular & cellular proteomics : MCP, [PMID:22223895] |
| 14. Van Damme, Petra P and 15 more authors.. (2012) N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.. Proceedings of the National Academy of Sciences of the United States of America, (31): [PMID:22814378] |
| 15. Folco, Eric G EG, Lee, Chung-Sheng CS, Dufu, Kobina K, Yamazaki, Tomohiro T and Reed, Robin R.. (2012) The proteins PDIP3 and ZC11A associate with the human TREX complex in an ATP-dependent manner and function in mRNA export.. PloS one, [PMID:22928037] |
| 16. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
| 17. Guo, Ailan and 15 more authors.. (2014) Immunoaffinity enrichment and mass spectrometry analysis of protein methylation.. Molecular & cellular proteomics : MCP, [PMID:24129315] |
