Basic Description

Product Name	P4HB Antibody - Primary antibody, specific to P4HB, Rabbit IgG
Synonyms	Cellular thyroid hormone-binding protein \| collagen prolyl 4-hydroxylase beta \| DSI \| ERBA2L \| GIT \| glutathione-insulin transhydrogenase \| P4HB \| P4Hbeta \| p55 \| PDI \| PDIA1 \| PDIA1procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase)
Specifications & Purity	ExactAb™, Validated, Carrier Free, Azide Free, Lot by Lot
Host species	Rabbit
Specificity	P4HB
Immunogen	Recombinant PDI expressed in E.coli (AA 216-465)
Positive Control	WB: Hep G2, MCF7, HEK293, A431 and HeLa cell lysates.
Conjugation	Unconjugated
Grade	Azide Free, Carrier Free, ExactAb™, Validated
Note	20μl卖完停产，不再备货
Product Description	Rabbit anti Human P4HB Antibody, Polyclonal, could be used for WB and so on. Application: WB: 0.5-2 µg/mL Protein Function This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP.

Product Properties

Isotype	Rabbit IgG
SDS-PAGE	150kDa
Purification Method	Antigen affinity purified and protein A purified
Form	Liquid
Concentration	Lot by Lot
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

P4HB Antibody (Ab119767) - Western Blot
All lanes: P4HB Antibody (Ab119767) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 57 kDa
Observed band size: 59 kDa
Exposure time: 3.4 s

Associated Targets(Human)

P4HB Tchem Protein disulfide-isomerase (0 Activities)

Discover Target: P4HB

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	References

Application

Application	Dilution info
WB	0.5-2 µg/mL

Certificates

Certificate of Analysis(COA)

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2 results found

Lot Number	Certificate Type	Date	Item
ZJ23F1001373	Certificate of Analysis	Oct 18, 2023	Ab119767
ZJ23F1001374	Certificate of Analysis	Oct 18, 2023	Ab119767

FAQs and Articles

References

1. Tan L, Gurbani D, Weisberg EL, Jones DS, Rao S, Singer WD, Bernard FM, Mowafy S, Jenney A, Du G et al.. (2017) Studies of TAK1-centered polypharmacology with novel covalent TAK1 inhibitors.. Bioorg Med Chem, 25 (4): (1320-1328). [PMID:28038940] [10.1021/op500134e]
2. Kaplan A, Stockwell BR.. (2015) Structural Elucidation of a Small Molecule Inhibitor of Protein Disulfide Isomerase.. ACS Med Chem Lett, 6 (9): (966-971). [PMID:26500720] [10.1021/acsmedchemlett.5b00014]
3. Hayes KE, Batsomboon P, Chen WC, Johnson BD, Becker A, Eschrich S, Yang Y, Robart AR, Dudley GB, Geldenhuys WJ, Hazlehurst LA.. (2019) Inhibition of the FAD containing ER oxidoreductin 1 (Ero1) protein by EN-460 as a strategy for treatment of multiple myeloma.. Bioorg Med Chem, 27 (8): (1479-1488). [PMID:30850265] [10.1016/j.bmc.2019.02.016]
4. Hochstrasser, D F DF and 9 more authors.. (1992) Human liver protein map: a reference database established by microsequencing and gel comparison.. Electrophoresis, [PMID:1286669]
5. Tasanen, K K, Oikarinen, J J, Kivirikko, K I KI and Pihlajaniemi, T T.. (1992) Promoter of the gene for the multifunctional protein disulfide isomerase polypeptide. Functional significance of the six CCAAT boxes and other promoter elements.. The Journal of biological chemistry, (5): [PMID:1597478]
6. Bauw, G G and 7 more authors.. (1990) Two-dimensional gel electrophoresis, protein electroblotting and microsequencing: a direct link between proteins and genes.. Electrophoresis, [PMID:1699755]
7. Ward, L D LD, Hong, J J, Whitehead, R H RH and Simpson, R J RJ.. (1990) Development of a database of amino acid sequences for human colon carcinoma proteins separated by two-dimensional polyacrylamide gel electrophoresis.. Electrophoresis, [PMID:2079031]
8. Tasanen, K K, Parkkonen, T T, Chow, L T LT, Kivirikko, K I KI and Pihlajaniemi, T T.. (1988) Characterization of the human gene for a polypeptide that acts both as the beta subunit of prolyl 4-hydroxylase and as protein disulfide isomerase.. The Journal of biological chemistry, (5): [PMID:2846539]
9. Pihlajaniemi, T T and 6 more authors.. (1987) Molecular cloning of the beta-subunit of human prolyl 4-hydroxylase. This subunit and protein disulphide isomerase are products of the same gene.. The EMBO journal, [PMID:3034602]
10. Morris, J I JI and Varandani, P T PT.. (1988) Characterization of a cDNA for human glutathione-insulin transhydrogenase (protein-disulfide isomerase/oxidoreductase).. Biochimica et biophysica acta, (28): [PMID:3342239]
11. Cheng, S Y SY and 6 more authors.. (1987) The nucleotide sequence of a human cellular thyroid hormone binding protein present in endoplasmic reticulum.. The Journal of biological chemistry, (15): [PMID:3611107]
12. Helaakoski, T T and 5 more authors.. (1995) Cloning, baculovirus expression, and characterization of a second mouse prolyl 4-hydroxylase alpha-subunit isoform: formation of an alpha 2 beta 2 tetramer with the protein disulfide-isomerase/beta subunit.. Proceedings of the National Academy of Sciences of the United States of America, (9): [PMID:7753822]
13. Kemmink, J J, Darby, N J NJ, Dijkstra, K K, Scheek, R M RM and Creighton, T E TE.. (1995) Nuclear magnetic resonance characterization of the N-terminal thioredoxin-like domain of protein disulfide isomerase.. Protein science : a publication of the Protein Society, [PMID:8580850]
14. Kemmink, J J, Darby, N J NJ, Dijkstra, K K, Nilges, M M and Creighton, T E TE.. (1996) Structure determination of the N-terminal thioredoxin-like domain of protein disulfide isomerase using multidimensional heteronuclear 13C/15N NMR spectroscopy.. Biochemistry, (18): [PMID:8672469]
15. Ji, H H and 5 more authors.. () A two-dimensional gel database of human colon carcinoma proteins.. Electrophoresis, [PMID:9150948]
16. Urade, R R and 5 more authors.. (1997) Functions of characteristic Cys-Gly-His-Cys (CGHC) and Gln-Glu-Asp-Leu (QEDL) motifs of microsomal ER-60 protease.. Journal of biochemistry, [PMID:9399589]
17. Kemmink, J J and 6 more authors.. (1999) The structure in solution of the b domain of protein disulfide isomerase.. Journal of biomolecular NMR, [PMID:10383197]
18. Mezghrani, A A and 5 more authors.. (2000) Protein-disulfide isomerase (PDI) in FRTL5 cells. pH-dependent thyroglobulin/PDI interactions determine a novel PDI function in the post-endoplasmic reticulum of thyrocytes.. The Journal of biological chemistry, (21): [PMID:10636893]
19. Fenouillet, E E, Barbouche, R R, Courageot, J J and Miquelis, R R.. (2001) The catalytic activity of protein disulfide isomerase is involved in human immunodeficiency virus envelope-mediated membrane fusion after CD4 cell binding.. The Journal of infectious diseases, (1): [PMID:11181151]
20. Mezghrani, A A and 5 more authors.. (2001) Manipulation of oxidative protein folding and PDI redox state in mammalian cells.. The EMBO journal, (15): [PMID:11707400]
21. Ko, Han Seok HS, Uehara, Takashi T and Nomura, Yasuyuki Y.. (2002) Role of ubiquilin associated with protein-disulfide isomerase in the endoplasmic reticulum in stress-induced apoptotic cell death.. The Journal of biological chemistry, (20): [PMID:12095988]
22. Gallina, Angelo A and 6 more authors.. (2002) Inhibitors of protein-disulfide isomerase prevent cleavage of disulfide bonds in receptor-bound glycoprotein 120 and prevent HIV-1 entry.. The Journal of biological chemistry, (27): [PMID:12218051]
23. Barbouche, Rym R, Miquelis, Raymond R, Jones, Ian M IM and Fenouillet, Emmanuel E.. (2003) Protein-disulfide isomerase-mediated reduction of two disulfide bonds of HIV envelope glycoprotein 120 occurs post-CXCR4 binding and is required for fusion.. The Journal of biological chemistry, (31): [PMID:12218052]
24. Lumb, Richard A RA and Bulleid, Neil J NJ.. (2002) Is protein disulfide isomerase a redox-dependent molecular chaperone?. The EMBO journal, (16): [PMID:12485997]
25. Basrur, Venkatesha V and 12 more authors.. () Proteomic analysis of early melanosomes: identification of novel melanosomal proteins.. Journal of proteome research, [PMID:12643545]
26. Gevaert, Kris K and 6 more authors.. (2003) Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides.. Nature biotechnology, [PMID:12665801]
27. She, Yi-Min YM and 5 more authors.. (2003) Identification of metal-binding proteins in human hepatoma lines by immobilized metal affinity chromatography and mass spectrometry.. Molecular & cellular proteomics : MCP, [PMID:14534351]
28. Markovic, Ingrid I and 8 more authors.. (2004) Thiol/disulfide exchange is a prerequisite for CXCR4-tropic HIV-1 envelope-mediated T-cell fusion during viral entry.. Blood, (1): [PMID:14592831]
29. Wilkinson, Bonney B and Gilbert, Hiram F HF.. (2004) Protein disulfide isomerase.. Biochimica et biophysica acta, (1): [PMID:15158710]
30. Barbouche, Rym R, Lortat-Jacob, Hugues H, Jones, Ian M IM and Fenouillet, Emmanuel E.. (2005) Glycosaminoglycans and protein disulfide isomerase-mediated reduction of HIV Env.. Molecular pharmacology, [PMID:15644496]
31. Chi, An A and 16 more authors.. (2006) Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes.. Journal of proteome research, [PMID:17081065]
32. Bi, Shuguang S, Hong, Patrick W PW, Lee, Benhur B and Baum, Linda G LG.. (2011) Galectin-9 binding to cell surface protein disulfide isomerase regulates the redox environment to enhance T-cell migration and HIV entry.. Proceedings of the National Academy of Sciences of the United States of America, (28): [PMID:21670307]
33. Khatun, Irani I, Walsh, Meghan T MT and Hussain, M Mahmood MM.. (2013) Loss of both phospholipid and triglyceride transfer activities of microsomal triglyceride transfer protein in abetalipoproteinemia.. Journal of lipid research, [PMID:23475612]
34. Ge, Jingyan J and 7 more authors.. (2013) Small molecule probe suitable for in situ profiling and inhibition of protein disulfide isomerase.. ACS chemical biology, (15): [PMID:24070012]
35. Rauch, Frank F and 8 more authors.. (2015) Cole-Carpenter syndrome is caused by a heterozygous missense mutation in P4HB.. American journal of human genetics, (5): [PMID:25683117]
36. Tagliabracci, Vincent S VS and 14 more authors.. (2015) A Single Kinase Generates the Majority of the Secreted Phosphoproteome.. Cell, (18): [PMID:26091039]
37. Walsh, Meghan T MT and 8 more authors.. (2015) Novel Abetalipoproteinemia Missense Mutation Highlights the Importance of the N-Terminal β-Barrel in Microsomal Triglyceride Transfer Protein Function.. Circulation. Cardiovascular genetics, [PMID:26224785]

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SKU	Size	Availability	Price
Ab119767-10μl	10μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$66.90
Ab119767-50μl	50μl	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$199.90
Ab119767-100μl	100μl	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$299.90
Ab119767-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$2,599.90

P4HB Antibody - Primary antibody, specific to P4HB, Rabbit IgG