mAb(J11); Mouse anti Human PAI1 Antibody; WB, IHC, IF, ICC; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab120074-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $69.90 | |
| Ab120074-20μl | 20μl | In stock | $99.90 | |
| Ab120074-50μl | 50μl | In stock | $199.90 | |
| Ab120074-100μl | 100μl | In stock | $299.90 | |
| Ab120074-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,299.90 |
mAb(J11); Mouse anti Human PAI1 Antibody; WB, IHC, IF, ICC; Unconjugated
| Product Name | PAI1 Mouse mAb - Primary antibody, specific to SERPINE1, Mouse IgG1 |
|---|---|
| Synonyms | Endothelial plasminogen activator inhibitor; Nexin; PAI1; PAI-1; PAI1PAI-1; PAISerpin E1; PLANH1; PLANH1plasminogen activator inhibitor 1; serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogenactivator inhibitor type 1), member 1; Serpin |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Azide Free, High performance, Lot by Lot |
| Host species | Mouse |
| Specificity | SERPINE1 |
| Immunogen | Recombinant PAI1 expressed in E.coli (AA 24-402). |
| Positive Control | WB: HeLa cell lysate. IHC: Human kidney tissue. IF/ICC: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, High Performance, Validated |
| Product Description | Mouse anti Human PAI1 Antibody, Monoclonal(J11), could be used for WB, IHC, IF, ICC and so on . Application: WB: 0.01-2µg/mL IHC: 5-20µg/mL IF/ICC: 5-20µg/mL Protein Function This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis. |
| Isotype | Mouse IgG1 |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A + Protein G purified |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
PAI1 Mouse mAb (Ab120074) - Western Blot
All lanes: PAI1 Mouse mAb (Ab120074) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 45 kDa
Observed band size: 47 kDa
Exposure time: 20.5 s
PAI1 Mouse mAb (Ab120074) - IF/ICC
FITC staining on IF
Sample: Human HeLa cells
Primary Ab: 20 μg/mL PAI1 Mouse mAb (Ab120074)
Second Ab: 5 μg/mL FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
PAI1 Mouse mAb (Ab120074) - IHC
DAB staining on IHC
Sample: Human Kidney Tissue
Primary Ab: 20 μg/mL PAI1 Mouse mAb (Ab120074)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| IHC | 5-20 µg/mL |
| WB |
0.01-2 µg/mL
|
| IF/ICC | 5-20µg/mL |
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F1201908 | Certificate of Analysis | Dec 06, 2023 | Ab120074 |
| ZJ23F1201907 | Certificate of Analysis | Dec 06, 2023 | Ab120074 |
| ZJ23F1201906 | Certificate of Analysis | Dec 06, 2023 | Ab120074 |
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