| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab120346-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $56.90 | |
| Ab120346-50μl | 50μl | In stock | $169.90 | |
| Ab120346-100μl | 100μl | In stock | $269.90 | |
| Ab120346-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $1,699.90 |
pAb; Rabbit anti Human PARK7 Antibody; WB, IHC; Unconjugated
| Product Name | PARK7 Antibody - Primary antibody, specific to PARK7, Rabbit IgG |
|---|---|
| Synonyms | CAP1 antibody | DJ-1 antibody | DJ1 antibody | DJ1 proteinantibody | Epididymis secretory sperm binding protein Li 67p antibody | FLJ27376 antibody | FLJ34360 antibody | FLJ92274 antibody | HEL S 67p antibody | Oncogene DJ1 antibody | OTTHUMP00000001348 a |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Azide Free, High performance, Lot by Lot |
| Host species | Rabbit |
| Specificity | PARK7 |
| Immunogen | Recombinant PARK7 expressed in E.coli (AA 1-189). |
| Positive Control | WB: Jurkat, MCF7, Hep G2, HeLa, HUVEC and NIH/3T3 cell lysates. IHC: Human spleen, kidney, skeletal muscle tissues. |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, High Performance, Validated |
| Note | 20μl卖完停产，不再备货 |
| Product Description | Rabbit anti Human PARK7 Antibody, Polyclonal antibody, could be used for WB, IHC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen-specific affinity chromatography followed by Protein A affinity chromatography |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
PARK7 Antibody (Ab120346) - Western Blot
All lanes: PARK7 Antibody (Ab120346) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 22 kDa
Observed band size: 23 kDa
Exposure time: 15.0 s
PARK7 Antibody (Ab120346) - IHC
DAB staining on IHC
Samples: Human Spleen Tissue
Primary Ab: 20 μg/mL PARK7 Antibody (Ab120346)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PARK7 Antibody (Ab120346) - IHC
DAB staining on IHC
Samples: Human Kidney Tissue
Primary Ab: 20 μg/mL PARK7 Antibody (Ab120346)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PARK7 Antibody (Ab120346) - IHC
DAB staining on IHC
Sample: Human Kidney Tissue
Primary Ab: 20 µg/mL PARK7 Antibody (Ab120346)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PARK7 Antibody (Ab120346) - IHC
DAB staining on IHC
Samples: Human Skeletal muscle Tissue
Primary Ab: 20 μg/mL PARK7 Antibody (Ab120346)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PARK7 Antibody (Ab120346) - IHC
DAB staining on IHC
Sample: Human Spleen Tissue
Primary Ab: 20 µg/mL PARK7 Antibody (Ab120346)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PARK7 Antibody (Ab120346) - IHC
DAB staining on IHC
Sample: Human Skeletal muscle Tissue
Primary Ab: 20 µg/mL PARK7 Antibody (Ab120346)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
| Application | Dilution info |
|---|---|
| IHC | 5-20 µg/mL |
| WB |
0.01-2 µg/mL
|
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F1101596 | Certificate of Analysis | Nov 08, 2023 | Ab120346 |
| ZJ23F1101597 | Certificate of Analysis | Nov 08, 2023 | Ab120346 |
| 1. Ju-young Lee,Jeeyeon Song,Kyu Kwon,Sumi Jang,Chayeon Kim,Kwanghee Baek,Jeongho Kim,Chankyu Park. (2012-04-24) Human DJ-1 and its homologs are novel glyoxalases.. Human molecular genetics, 21 ((14)): (3215-3225). [PMID:22523093] |
| 2. Nagakubo, D D and 6 more authors.. (1997) DJ-1, a novel oncogene which transforms mouse NIH3T3 cells in cooperation with ras.. Biochemical and biophysical research communications, (13): [PMID:9070310] |
| 3. Taira, T T, Takahashi, K K, Kitagawa, R R, Iguchi-Ariga, S M SM and Ariga, H H.. (2001) Molecular cloning of human and mouse DJ-1 genes and identification of Sp1-dependent activation of the human DJ-1 promoter.. Gene, (24): [PMID:11223268] |
| 4. Takahashi, K K and 5 more authors.. (2001) DJ-1 positively regulates the androgen receptor by impairing the binding of PIASx alpha to the receptor.. The Journal of biological chemistry, (5): [PMID:11477070] |
| 5. Bonifati, Vincenzo V and 17 more authors.. (2003) Mutations in the DJ-1 gene associated with autosomal recessive early-onset parkinsonism.. Science (New York, N.Y.), (10): [PMID:12446870] |
| 6. Niki, Takeshi T, Takahashi-Niki, Kazuko K, Taira, Takahiro T, Iguchi-Ariga, Sanae M M SM and Ariga, Hiroyoshi H.. (2003) DJBP: a novel DJ-1-binding protein, negatively regulates the androgen receptor by recruiting histone deacetylase complex, and DJ-1 antagonizes this inhibition by abrogation of this complex.. Molecular cancer research : MCR, [PMID:12612053] |
| 7. Tao, Xiao X and Tong, Liang L.. (2003) Crystal structure of human DJ-1, a protein associated with early onset Parkinson's disease.. The Journal of biological chemistry, (15): [PMID:12761214] |
| 8. Honbou, Kazuya K and 6 more authors.. (2003) The crystal structure of DJ-1, a protein related to male fertility and Parkinson's disease.. The Journal of biological chemistry, (15): [PMID:12796482] |
| 9. Miller, David W DW and 12 more authors.. (2003) L166P mutant DJ-1, causative for recessive Parkinson's disease, is degraded through the ubiquitin-proteasome system.. The Journal of biological chemistry, (19): [PMID:12851414] |
| 10. Wilson, Mark A MA, Collins, Jennifer L JL, Hod, Yaacov Y, Ringe, Dagmar D and Petsko, Gregory A GA.. (2003) The 1.1-A resolution crystal structure of DJ-1, the protein mutated in autosomal recessive early onset Parkinson's disease.. Proceedings of the National Academy of Sciences of the United States of America, (5): [PMID:12855764] |
| 11. Huai, Qing Q and 6 more authors.. (2003) Crystal structure of DJ-1/RS and implication on familial Parkinson's disease.. FEBS letters, (14): [PMID:12914946] |
| 12. Lee, Sun-Joo SJ and 10 more authors.. (2003) Crystal structures of human DJ-1 and Escherichia coli Hsp31, which share an evolutionarily conserved domain.. The Journal of biological chemistry, (7): [PMID:12939276] |
| 13. Abou-Sleiman, Patrick M PM, Healy, Daniel G DG, Quinn, Niall N, Lees, Andrew J AJ and Wood, Nicholas W NW.. (2003) The role of pathogenic DJ-1 mutations in Parkinson's disease.. Annals of neurology, [PMID:12953260] |
| 14. Yoshida, Kaoru K and 5 more authors.. (2003) Immunocytochemical localization of DJ-1 in human male reproductive tissue.. Molecular reproduction and development, [PMID:14579415] |
| 15. Görner, Karin K and 8 more authors.. (2004) Differential effects of Parkinson's disease-associated mutations on stability and folding of DJ-1.. The Journal of biological chemistry, (20): [PMID:14607841] |
| 16. Bandopadhyay, Rina R and 20 more authors.. (2004) The expression of DJ-1 (PARK7) in normal human CNS and idiopathic Parkinson's disease.. Brain : a journal of neurology, [PMID:14662519] |
| 17. Rizzu, Patrizia P and 11 more authors.. (2004) DJ-1 colocalizes with tau inclusions: a link between parkinsonism and dementia.. Annals of neurology, [PMID:14705119] |
| 18. Hedrich, Katja K and 15 more authors.. (2004) The R98Q variation in DJ-1 represents a rare polymorphism.. Annals of neurology, [PMID:14705128] |
| 19. Moore, Darren J DJ, Zhang, Li L, Dawson, Ted M TM and Dawson, Valina L VL.. (2003) A missense mutation (L166P) in DJ-1, linked to familial Parkinson's disease, confers reduced protein stability and impairs homo-oligomerization.. Journal of neurochemistry, [PMID:14713311] |
| 20. Taira, Takahiro T and 5 more authors.. (2004) DJ-1 has a role in antioxidative stress to prevent cell death.. EMBO reports, [PMID:14749723] |
| 21. Hedrich, K K and 17 more authors.. (2004) DJ-1 (PARK7) mutations are less frequent than Parkin (PARK2) mutations in early-onset Parkinson disease.. Neurology, (10): [PMID:14872018] |
| 22. Canet-Avilés, Rosa M RM and 9 more authors.. (2004) The Parkinson's disease protein DJ-1 is neuroprotective due to cysteine-sulfinic acid-driven mitochondrial localization.. Proceedings of the National Academy of Sciences of the United States of America, (15): [PMID:15181200] |
| 23. Clark, Lorraine N LN and 16 more authors.. (2004) Analysis of an early-onset Parkinson's disease cohort for DJ-1 mutations.. Movement disorders : official journal of the Movement Disorder Society, [PMID:15254937] |
| 24. Hering, Robert R and 18 more authors.. (2004) Novel homozygous p.E64D mutation in DJ1 in early onset Parkinson disease (PARK7).. Human mutation, [PMID:15365989] |
| 25. Shendelman, Shoshana S, Jonason, Alan A, Martinat, Cecile C, Leete, Thomas T and Abeliovich, Asa A.. (2004) DJ-1 is a redox-dependent molecular chaperone that inhibits alpha-synuclein aggregate formation.. PLoS biology, [PMID:15502874] |
| 26. Rush, John J and 9 more authors.. (2005) Immunoaffinity profiling of tyrosine phosphorylation in cancer cells.. Nature biotechnology, [PMID:15592455] |
| 27. Shinbo, Y Y and 8 more authors.. (2006) Proper SUMO-1 conjugation is essential to DJ-1 to exert its full activities.. Cell death and differentiation, [PMID:15976810] |
| 28. Annesi, Grazia G and 15 more authors.. (2005) DJ-1 mutations and parkinsonism-dementia-amyotrophic lateral sclerosis complex.. Annals of neurology, [PMID:16240358] |
| 29. Sekito, Aya A and 5 more authors.. (2006) DJ-1 interacts with HIPK1 and affects H2O2-induced cell death.. Free radical research, [PMID:16390825] |
| 30. Tang, Beisha B and 11 more authors.. (2006) Association of PINK1 and DJ-1 confers digenic inheritance of early-onset Parkinson's disease.. Human molecular genetics, (1): [PMID:16632486] |
| 31. Gregory, S G SG and 178 more authors.. (2006) The DNA sequence and biological annotation of human chromosome 1.. Nature, (18): [PMID:16710414] |
| 32. Clements, Casey M CM, McNally, Richard S RS, Conti, Brian J BJ, Mak, Tak W TW and Ting, Jenny P-Y JP.. (2006) DJ-1, a cancer- and Parkinson's disease-associated protein, stabilizes the antioxidant transcriptional master regulator Nrf2.. Proceedings of the National Academy of Sciences of the United States of America, (10): [PMID:17015834] |
| 33. Olzmann, James A JA and 6 more authors.. (2007) Parkin-mediated K63-linked polyubiquitination targets misfolded DJ-1 to aggresomes via binding to HDAC6.. The Journal of cell biology, (10): [PMID:17846173] |
| 34. van der Brug, Marcel P MP and 18 more authors.. (2008) RNA binding activity of the recessive parkinsonism protein DJ-1 supports involvement in multiple cellular pathways.. Proceedings of the National Academy of Sciences of the United States of America, (22): [PMID:18626009] |
| 35. Junn, Eunsung E, Jang, Won Hee WH, Zhao, Xin X, Jeong, Byeong Seon BS and Mouradian, M Maral MM.. (2009) Mitochondrial localization of DJ-1 leads to enhanced neuroprotection.. Journal of neuroscience research, [PMID:18711745] |
| 36. Guo, Ji-Feng JF and 11 more authors.. (2008) Mutation analysis of Parkin, PINK1, DJ-1 and ATP13A2 genes in Chinese patients with autosomal recessive early-onset Parkinsonism.. Movement disorders : official journal of the Movement Disorder Society, (30): [PMID:18785233] |
| 37. Xiong, Hui H and 10 more authors.. (2009) Parkin, PINK1, and DJ-1 form a ubiquitin E3 ligase complex promoting unfolded protein degradation.. The Journal of clinical investigation, [PMID:19229105] |
| 38. Krebiehl, Guido G and 13 more authors.. (2010) Reduced basal autophagy and impaired mitochondrial dynamics due to loss of Parkinson's disease-associated protein DJ-1.. PloS one, (23): [PMID:20186336] |
| 39. Chen, Jue J, Li, Lian L and Chin, Lih-Shen LS.. (2010) Parkinson disease protein DJ-1 converts from a zymogen to a protease by carboxyl-terminal cleavage.. Human molecular genetics, (15): [PMID:20304780] |
| 40. McNally, R Sean RS and 5 more authors.. (2011) DJ-1 enhances cell survival through the binding of Cezanne, a negative regulator of NF-kappaB.. The Journal of biological chemistry, (11): [PMID:21097510] |
| 41. Jain, Deepak D and 7 more authors.. (2012) Age- and diet-dependent requirement of DJ-1 for glucose homeostasis in mice with implications for human type 2 diabetes.. Journal of molecular cell biology, [PMID:22611253] |
| 42. Björkblom, Benny B and 8 more authors.. (2013) Parkinson disease protein DJ-1 binds metals and protects against metal-induced cytotoxicity.. The Journal of biological chemistry, (2): [PMID:23792957] |
| 43. Kim, Kwang Soo KS and 6 more authors.. (2013) DJ-1 associates with lipid rafts by palmitoylation and regulates lipid rafts-dependent endocytosis in astrocytes.. Human molecular genetics, (1): [PMID:23847046] |
| 44. Richarme, Gilbert G and 5 more authors.. (2015) Parkinsonism-associated protein DJ-1/Park7 is a major protein deglycase that repairs methylglyoxal- and glyoxal-glycated cysteine, arginine, and lysine residues.. The Journal of biological chemistry, (16): [PMID:25416785] |
| 45. Hanagasi, Hasmet A HA and 11 more authors.. (2016) A novel homozygous DJ1 mutation causes parkinsonism and ALS in a Turkish family.. Parkinsonism & related disorders, [PMID:26972524] |
| 46. Advedissian, Tamara T, Deshayes, Frédérique F, Poirier, Françoise F, Viguier, Mireille M and Richarme, Gilbert G.. (2016) The Parkinsonism-associated protein DJ-1/Park7 prevents glycation damage in human keratinocyte.. Biochemical and biophysical research communications, (22): [PMID:26995087] |
| 47. Pfaff, Daniel H DH, Fleming, Thomas T, Nawroth, Peter P and Teleman, Aurelio A AA.. (2017) Evidence Against a Role for the Parkinsonism-associated Protein DJ-1 in Methylglyoxal Detoxification.. The Journal of biological chemistry, (13): [PMID:27903648] |
| 48. Richarme, Gilbert G and Dairou, Julien J.. (2017) Parkinsonism-associated protein DJ-1 is a bona fide deglycase.. Biochemical and biophysical research communications, (29): [PMID:28013050] |
| 49. Richarme, Gilbert G and 11 more authors.. (2017) Guanine glycation repair by DJ-1/Park7 and its bacterial homologs.. Science (New York, N.Y.), (14): [PMID:28596309] |
| 50. Matsuda, Noriyuki N and 10 more authors.. (2017) Parkinson's disease-related DJ-1 functions in thiol quality control against aldehyde attack in vitro.. Scientific reports, (9): [PMID:28993701] |
| 51. Galligan, James J and 10 more authors.. (2018) Methylglyoxal-derived posttranslational arginine modifications are abundant histone marks.. Proceedings of the National Academy of Sciences of the United States of America, (11): [PMID:30150385] |
| 52. Zheng, Qingfei Q and 10 more authors.. (2019) Reversible histone glycation is associated with disease-related changes in chromatin architecture.. Nature communications, (20): [PMID:30894531] |
| 53. Stephenson, Sarah Em SE and 6 more authors.. (2019) Familial early onset Parkinson's disease caused by a homozygous frameshift variant in PARK7: Clinical features and literature update.. Parkinsonism & related disorders, (22): [PMID:30928208] |
| 54. Moutaoufik, Mohamed Taha and 27 more authors.. (2019) Rewiring of the Human Mitochondrial Interactome during Neuronal Reprogramming Reveals Regulators of the Respirasome and Neurogenesis.. iScience, (27): [PMID:31536960] |
| 55. Andreeva, Anna and 7 more authors.. (2019) The apparent deglycase activity of DJ-1 results from the conversion of free methylglyoxal present in fast equilibrium with hemithioacetals and hemiaminals.. The Journal of biological chemistry, (6): [PMID:31653696] |
