mAb (3D7); Mouse anti Human PCNA Antibody; WB, Flow, ICC/IF, ELISA; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab155826-10μl (Trial Size) Apply for free trial size（?） Every year, as a valued customer, you have the exclusive opportunity to explore and enjoy three different trial products of your choice, absolutely free! | 10μl | In stock | $69.90 | |
| Ab155826-20μl | 20μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $99.90 | |
| Ab155826-50μl | 50μl | In stock | $201.90 | |
| Ab155826-100μl | 100μl | In stock | $300.90 | |
| Ab155826-1ml | 1ml | In stock | $1,500.90 |
mAb (3D7); Mouse anti Human PCNA Antibody; WB, Flow, ICC/IF, ELISA; Unconjugated
| Product Name | PCNA Mouse mAb - Primary antibody, for WB,Flow,ELISA,ICC/IF various applications, from Human, generated in Mouse, specific to PCNA, IgG1 |
|---|---|
| Synonyms | ATLD2 antibody; cb16 antibody; Cyclin antibody; DNA polymerase delta auxiliary protein antibody; etID36690.10 antibody; fa28e03 antibody; fb36g03 antibody; HGCN8729 antibody; MGC8367 antibody; Mutagen-sensitive 209 protein antibody; OTTHUMP00000030189 ant |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Azide Free, High performance, Lot to lot |
| Host Species | Mouse |
| Host species | Mouse |
| Specificity | PCNA |
| Application | WB,Flow,ELISA,ICC/IF |
| Immunogen | Full length recombinant human PCNA |
| Positive Control | WB: HeLa, A431, HEK293, HepG2, NIH3T3 and C6 whole cell lysates Flow: HeLa cells ICC/IF: HeLa cells |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, High performance, Validated |
| Product Description | Application
Protein Function: Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication. Crystal structure data suggests that a PCNA homotrimer ring can encircle and slide along the DNA double helix. Multiple proteins involved in DNA replication, DNA repair, and cell cycle control bind to PCNA rather than directly associating with DNA, thus facilitating fast processing of DNA. PCNA protein expression is a well-accepted marker of proliferation. |
| Antibody Type | Primary antibody |
|---|---|
| Clonality | Monoclonal antibodies |
| Isotype | IgG1 |
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Source | Hybridoma supernatant |
| Form | Liquid |
| Concentration | Lot to lot |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze/thaw cycle. |
PCNA Mouse mAb (Ab155826) - Western Blot
All lanes: PCNA Mouse mAb (Ab155826) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/10000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa
PCNA Mouse mAb (Ab155826) - ICC/IF
IF analysis of PCNA (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with PCNA Mouse mAb (Ab155826) at 1/2000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.
PCNA Mouse mAb (Ab155826) - Flow Cytometry
Flow analysis of PCNA (red line) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes. PCNA Mouse mAb (Ab155826) at 1/4000 dilution for 1 hour at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 30 minutes at 4°C in the dark . Unlabeled sample (black line) was used as a negative control.
PCNA Mouse mAb (Ab155826) - Western Blot
All lanes: PCNA Antibody (Ab155826) at 0.05 μg/mL
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 29.0 kDa
Observed band size: 32.0 kDa
Exposure time: 97.0 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| ICC/IF |
0.5 μg/mL
|
| Flow |
0.016-2.0μg/mL
|
| WB |
1/1,000-1/10,000 ; Predicted molecular weight: 29 kDa.
|
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0102565 | Certificate of Analysis | Jan 24, 2024 | Ab155826 |
| ZJ24F0102564 | Certificate of Analysis | Jan 24, 2024 | Ab155826 |
| ZJ24F0102563 | Certificate of Analysis | Jan 24, 2024 | Ab155826 |
| ZJ24F0102562 | Certificate of Analysis | Jan 24, 2024 | Ab155826 |
| ZJ23F0700606 | Certificate of Analysis | Jul 14, 2023 | Ab155826 |
| ZJ23F0700607 | Certificate of Analysis | Jul 14, 2023 | Ab155826 |
| 1. Francis Impens,Lilliana Radoshevich,Pascale Cossart,David Ribet. (2014-08-13) Mapping of SUMO sites and analysis of SUMOylation changes induced by external stimuli.. Proceedings of the National Academy of Sciences of the United States of America, 111 ((34)): (12432-12437). [PMID:25114211] |
| 2. Travali, S S and 5 more authors.. (1989) Structure of the human gene for the proliferating cell nuclear antigen.. The Journal of biological chemistry, (5): [PMID:2565339] |
| 3. Prelich, G G, Kostura, M M, Marshak, D R DR, Mathews, M B MB and Stillman, B B.. (1987) The cell-cycle regulated proliferating cell nuclear antigen is required for SV40 DNA replication in vitro.. Nature, [PMID:2882422] |
| 4. Gulbis, J M JM, Kelman, Z Z, Hurwitz, J J, O'Donnell, M M and Kuriyan, J J.. (1996) Structure of the C-terminal region of p21(WAF1/CIP1) complexed with human PCNA.. Cell, (18): [PMID:8861913] |
| 5. Chuang, L S LS and 5 more authors.. (1997) Human DNA-(cytosine-5) methyltransferase-PCNA complex as a target for p21WAF1.. Science (New York, N.Y.), (26): [PMID:9302295] |
| 6. Gary, R R, Ludwig, D L DL, Cornelius, H L HL, MacInnes, M A MA and Park, M S MS.. (1997) The DNA repair endonuclease XPG binds to proliferating cell nuclear antigen (PCNA) and shares sequence elements with the PCNA-binding regions of FEN-1 and cyclin-dependent kinase inhibitor p21.. The Journal of biological chemistry, (26): [PMID:9305916] |
| 7. Saha, P P and 7 more authors.. (1998) Human CDC6/Cdc18 associates with Orc1 and cyclin-cdk and is selectively eliminated from the nucleus at the onset of S phase.. Molecular and cellular biology, [PMID:9566895] |
| 8. Law, B K BK and 6 more authors.. (2000) Salicylate-induced growth arrest is associated with inhibition of p70s6k and down-regulation of c-myc, cyclin D1, cyclin A, and proliferating cell nuclear antigen.. The Journal of biological chemistry, (8): [PMID:10993886] |
| 9. Tsuchimoto, D D and 6 more authors.. (2001) Human APE2 protein is mostly localized in the nuclei and to some extent in the mitochondria, while nuclear APE2 is partly associated with proliferating cell nuclear antigen.. Nucleic acids research, (1): [PMID:11376153] |
| 10. Ducoux, M M and 6 more authors.. (2001) Mediation of proliferating cell nuclear antigen (PCNA)-dependent DNA replication through a conserved p21(Cip1)-like PCNA-binding motif present in the third subunit of human DNA polymerase delta.. The Journal of biological chemistry, (28): [PMID:11595739] |
| 11. Haracska, Lajos L and 6 more authors.. (2002) Stimulation of DNA synthesis activity of human DNA polymerase kappa by PCNA.. Molecular and cellular biology, [PMID:11784855] |
| 12. Liu, Li L, Rodriguez-Belmonte, Esther M EM, Mazloum, Nayef N, Xie, Bin B and Lee, Marietta Y W T MY.. (2003) Identification of a novel protein, PDIP38, that interacts with the p50 subunit of DNA polymerase delta and proliferating cell nuclear antigen.. The Journal of biological chemistry, (21): [PMID:12522211] |
| 13. Fujita, Kiyoko K and 9 more authors.. (2003) Terminal deoxynucleotidyltransferase forms a ternary complex with a novel chromatin remodeling protein with 82 kDa and core histone.. Genes to cells : devoted to molecular & cellular mechanisms, [PMID:12786946] |
| 14. Kannouche, Patricia L PL, Wing, Jonathan J and Lehmann, Alan R AR.. (2004) Interaction of human DNA polymerase eta with monoubiquitinated PCNA: a possible mechanism for the polymerase switch in response to DNA damage.. Molecular cell, (21): [PMID:15149598] |
| 15. Dzantiev, Leonid L and 5 more authors.. (2004) A defined human system that supports bidirectional mismatch-provoked excision.. Molecular cell, (2): [PMID:15225546] |
| 16. Poot, Raymond A RA and 8 more authors.. (2004) The Williams syndrome transcription factor interacts with PCNA to target chromatin remodelling by ISWI to replication foci.. Nature cell biology, [PMID:15543136] |
| 17. Sakurai, Shigeru S and 9 more authors.. (2005) Structural basis for recruitment of human flap endonuclease 1 to PCNA.. The EMBO journal, (23): [PMID:15616578] |
| 18. Li, Hao H and 8 more authors.. (2006) Functional roles of p12, the fourth subunit of human DNA polymerase delta.. The Journal of biological chemistry, (26): [PMID:16510448] |
| 19. Jin, Jianping J, Arias, Emily E EE, Chen, Jing J, Harper, J Wade JW and Walter, Johannes C JC.. (2006) A family of diverse Cul4-Ddb1-interacting proteins includes Cdt2, which is required for S phase destruction of the replication factor Cdt1.. Molecular cell, (1): [PMID:16949367] |
| 20. Unk, Ildiko I and 9 more authors.. (2006) Human SHPRH is a ubiquitin ligase for Mms2-Ubc13-dependent polyubiquitylation of proliferating cell nuclear antigen.. Proceedings of the National Academy of Sciences of the United States of America, (28): [PMID:17108083] |
| 21. Wang, Shao-Chun SC and 10 more authors.. (2006) Tyrosine phosphorylation controls PCNA function through protein stability.. Nature cell biology, [PMID:17115032] |
| 22. Motegi, Akira A and 5 more authors.. (2006) Human SHPRH suppresses genomic instability through proliferating cell nuclear antigen polyubiquitination.. The Journal of cell biology, (4): [PMID:17130289] |
| 23. Unk, Ildiko I and 7 more authors.. (2008) Human HLTF functions as a ubiquitin ligase for proliferating cell nuclear antigen polyubiquitination.. Proceedings of the National Academy of Sciences of the United States of America, (11): [PMID:18316726] |
| 24. Farina, Andrea A and 6 more authors.. (2008) Studies with the human cohesin establishment factor, ChlR1. Association of ChlR1 with Ctf18-RFC and Fen1.. The Journal of biological chemistry, (25): [PMID:18499658] |
| 25. Nishitani, Hideo H and 5 more authors.. (2008) CDK inhibitor p21 is degraded by a proliferating cell nuclear antigen-coupled Cul4-DDB1Cdt2 pathway during S phase and after UV irradiation.. The Journal of biological chemistry, (24): [PMID:18703516] |
| 26. Motegi, Akira A and 10 more authors.. (2008) Polyubiquitination of proliferating cell nuclear antigen by HLTF and SHPRH prevents genomic instability from stalled replication forks.. Proceedings of the National Academy of Sciences of the United States of America, (26): [PMID:18719106] |
| 27. Abbas, Tarek T and 5 more authors.. (2008) PCNA-dependent regulation of p21 ubiquitylation and degradation via the CRL4Cdt2 ubiquitin ligase complex.. Genes & development, (15): [PMID:18794347] |
| 28. Zhang, Sufang S and 5 more authors.. (2008) PCNA is ubiquitinated by RNF8.. Cell cycle (Georgetown, Tex.), (1): [PMID:18948756] |
| 29. Yu, Yu Y and 13 more authors.. (2009) Proliferating cell nuclear antigen is protected from degradation by forming a complex with MutT Homolog2.. The Journal of biological chemistry, (17): [PMID:19419956] |
| 30. Burkovics, Peter P, Hajdú, Ildikó I, Szukacsov, Valéria V, Unk, Ildiko I and Haracska, Lajos L.. (2009) Role of PCNA-dependent stimulation of 3'-phosphodiesterase and 3'-5' exonuclease activities of human Ape2 in repair of oxidative DNA damage.. Nucleic acids research, [PMID:19443450] |
| 31. Moldovan, George-Lucian GL and 5 more authors.. (2010) DNA polymerase POLN participates in cross-link repair and homologous recombination.. Molecular and cellular biology, [PMID:19995904] |
| 32. Terai, Kenta K, Abbas, Tarek T, Jazaeri, Amir A AA and Dutta, Anindya A.. (2010) CRL4(Cdt2) E3 ubiquitin ligase monoubiquitinates PCNA to promote translesion DNA synthesis.. Molecular cell, (15): [PMID:20129063] |
| 33. Ogi, Tomoo T and 13 more authors.. (2010) Three DNA polymerases, recruited by different mechanisms, carry out NER repair synthesis in human cells.. Molecular cell, (12): [PMID:20227374] |
| 34. De Haro, Leyma P LP and 9 more authors.. (2010) Metnase promotes restart and repair of stalled and collapsed replication forks.. Nucleic acids research, [PMID:20457750] |
| 35. Groehler, Angela L AL and Lannigan, Deborah A DA.. (2010) A chromatin-bound kinase, ERK8, protects genomic integrity by inhibiting HDM2-mediated degradation of the DNA clamp PCNA.. The Journal of cell biology, (23): [PMID:20733054] |
| 36. Rowbotham, Samuel P SP and 12 more authors.. (2011) Maintenance of silent chromatin through replication requires SWI/SNF-like chromatin remodeler SMARCAD1.. Molecular cell, (6): [PMID:21549307] |
| 37. Emanuele, Michael J MJ, Ciccia, Alberto A, Elia, Andrew E H AE and Elledge, Stephen J SJ.. (2011) Proliferating cell nuclear antigen (PCNA)-associated KIAA0101/PAF15 protein is a cell cycle-regulated anaphase-promoting complex/cyclosome substrate.. Proceedings of the National Academy of Sciences of the United States of America, (14): [PMID:21628590] |
| 38. Rahmeh, Amal A AA and 5 more authors.. (2012) Phosphorylation of the p68 subunit of Pol δ acts as a molecular switch to regulate its interaction with PCNA.. Biochemistry, (10): [PMID:22148433] |
| 39. Moldovan, George-Lucian GL and 6 more authors.. (2012) Inhibition of homologous recombination by the PCNA-interacting protein PARI.. Molecular cell, (13): [PMID:22153967] |
| 40. Punchihewa, Chandanamali C and 14 more authors.. (2012) Identification of small molecule proliferating cell nuclear antigen (PCNA) inhibitor that disrupts interactions with PIP-box proteins and inhibits DNA replication.. The Journal of biological chemistry, (20): [PMID:22383522] |
| 41. Din, Farhat V N FV and 7 more authors.. (2012) Aspirin inhibits mTOR signaling, activates AMP-activated protein kinase, and induces autophagy in colorectal cancer cells.. Gastroenterology, [PMID:22406476] |
| 42. Arboleda, Valerie A VA and 14 more authors.. (2012) Mutations in the PCNA-binding domain of CDKN1C cause IMAGe syndrome.. Nature genetics, (27): [PMID:22634751] |
| 43. Centore, Richard C RC, Yazinski, Stephanie A SA, Tse, Alice A and Zou, Lee L.. (2012) Spartan/C1orf124, a reader of PCNA ubiquitylation and a regulator of UV-induced DNA damage response.. Molecular cell, (8): [PMID:22681887] |
| 44. Ciccia, Alberto A and 12 more authors.. (2012) Polyubiquitinated PCNA recruits the ZRANB3 translocase to maintain genomic integrity after replication stress.. Molecular cell, (10): [PMID:22704558] |
| 45. Yuan, Jingsong J, Ghosal, Gargi G and Chen, Junjie J.. (2012) The HARP-like domain-containing protein AH2/ZRANB3 binds to PCNA and participates in cellular response to replication stress.. Molecular cell, (10): [PMID:22705370] |
| 46. Weston, Ria R, Peeters, Hanneke H and Ahel, Dragana D.. (2012) ZRANB3 is a structure-specific ATP-dependent endonuclease involved in replication stress response.. Genes & development, (15): [PMID:22759634] |
| 47. Povlsen, Lou K LK and 9 more authors.. (2012) Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.. Nature cell biology, [PMID:23000965] |
| 48. Bacquin, Agathe A and 8 more authors.. (2013) The helicase FBH1 is tightly regulated by PCNA via CRL4(Cdt2)-mediated proteolysis in human cells.. Nucleic acids research, [PMID:23677613] |
| 49. Menning, Maureen M and Kufer, Thomas A TA.. (2013) A role for the Ankyrin repeat containing protein Ankrd17 in Nod1- and Nod2-mediated inflammatory responses.. FEBS letters, (11): [PMID:23711367] |
| 50. Vannier, Jean-Baptiste JB and 6 more authors.. (2013) RTEL1 is a replisome-associated helicase that promotes telomere and genome-wide replication.. Science (New York, N.Y.), (11): [PMID:24115439] |
| 51. Alabert, Constance C and 9 more authors.. (2014) Nascent chromatin capture proteomics determines chromatin dynamics during DNA replication and identifies unknown fork components.. Nature cell biology, [PMID:24561620] |
| 52. Nicolae, Claudia M CM and 6 more authors.. (2014) The ADP-ribosyltransferase PARP10/ARTD10 interacts with proliferating cell nuclear antigen (PCNA) and is required for DNA damage tolerance.. The Journal of biological chemistry, (9): [PMID:24695737] |
| 53. Baple, Emma L EL and 21 more authors.. (2014) Hypomorphic PCNA mutation underlies a human DNA repair disorder.. The Journal of clinical investigation, [PMID:24911150] |
| 54. Cazzalini, Ornella O and 11 more authors.. (2014) CBP and p300 acetylate PCNA to link its degradation with nucleotide excision repair synthesis.. Nucleic acids research, [PMID:24939902] |
| 55. Perry, J Jefferson P JJ and 5 more authors.. (2015) Human C6orf211 encodes Armt1, a protein carboxyl methyltransferase that targets PCNA and is linked to the DNA damage response.. Cell reports, (3): [PMID:25732820] |
| 56. Hendriks, Ivo A; Treffers, Louise W; Verlaan-de Vries, Matty; Olsen, Jesper V and Vertegaal, Alfred C O.. (2015) SUMO-2 Orchestrates Chromatin Modifiers in Response to DNA Damage.. Cell reports, (17): [PMID:25772364] |
| 57. Sun, Zhiguo Z, Jha, Hem Chandra HC and Robertson, Erle S ES.. (2015) Bub1 in Complex with LANA Recruits PCNA To Regulate Kaposi's Sarcoma-Associated Herpesvirus Latent Replication and DNA Translesion Synthesis.. Journal of virology, [PMID:26223641] |
| 58. Fu, Dragony D, Samson, Leona D LD, Hübscher, Ullrich U and van Loon, Barbara B.. (2015) The interaction between ALKBH2 DNA repair enzyme and PCNA is direct, mediated by the hydrophobic pocket of PCNA and perturbed in naturally-occurring ALKBH2 variants.. DNA repair, [PMID:26408825] |
| 59. Hoffmann, Saskia S and 13 more authors.. (2016) TRAIP is a PCNA-binding ubiquitin ligase that protects genome stability after replication stress.. The Journal of cell biology, (4): [PMID:26711499] |
| 60. Wang, Yong Y, Xu, Min M and Jiang, Tao T.. (2016) Crystal structure of human PCNA in complex with the PIP box of DVC1.. Biochemical and biophysical research communications, (27): [PMID:27084448] |
| 61. Feng, Wanjuan and 5 more authors.. (2016) TRAIP regulates replication fork recovery and progression via PCNA.. Cell discovery, [PMID:27462463] |
| 62. Jo, Ukhyun U and 5 more authors.. (2016) PCNA-Dependent Cleavage and Degradation of SDE2 Regulates Response to Replication Stress.. PLoS genetics, [PMID:27906959] |
| 63. Reynolds, John J JJ and 61 more authors.. (2017) Mutations in DONSON disrupt replication fork stability and cause microcephalic dwarfism.. Nature genetics, [PMID:28191891] |
| 64. Chen, Wanbiao W and 5 more authors.. (2017) Structure insights into the molecular mechanism of the interaction between UHRF2 and PCNA.. Biochemical and biophysical research communications, (16): [PMID:28951215] |
| 65. Kottemann, Molly C MC, Conti, Brooke A BA, Lach, Francis P FP and Smogorzewska, Agata A.. (2018) Removal of RTF2 from Stalled Replisomes Promotes Maintenance of Genome Integrity.. Molecular cell, (4): [PMID:29290612] |
| 66. Almendral, J M JM, Huebsch, D D, Blundell, P A PA, Macdonald-Bravo, H H and Bravo, R R.. (1987) Cloning and sequence of the human nuclear protein cyclin: homology with DNA-binding proteins.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2882507] |
