PDCD6/ALG-2 Antibody - Primary antibody, specific to PDCD6/ALG-2, Rabbit IgG

    Application:
  • Flow Cytometry
  • IF/ICC
  • IHC
  • WB
Features and benefits
  • Host species: Rabbit
  • Species reactivity(Reacts with): Human
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
Item Number
Ab120811
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab120811-10μl
10μl
In stock
$49.90
Ab120811-50μl
50μl
In stock
$149.90
Ab120811-100μl
100μl
In stock
$239.90
Ab120811-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,999.90

pAb; Rabbit anti Human PDCD6/ALG-2 Antibody; WB, IHC, ICC, IF, Flow; Unconjugated

Basic Description

Product NamePDCD6/ALG-2 Antibody - Primary antibody, specific to PDCD6/ALG-2, Rabbit IgG
SynonymsAIP1 antibody | ALG 2 antibody | ALG-2-interacting protein 1 antibody | ALG2 antibody | ALIX antibody | Apoptosis linked gene 2 antibody | Apoptosis linked gene 2 protein antibody | Apoptosis-linked gene 2 protein antibody | FLJ42309 antibody | FLJ46208 a
Specifications & PurityExactAb™, Validated, Carrier Free, Azide Free, High performance, Lot by Lot
Host speciesRabbit
SpecificityPDCD6/ALG-2
ImmunogenRecombinant PDCD6 expressed in E.coli (AA 32-191)
Positive ControlWB: Hep G2 and Jurkat cell lysates. IHC: Human kidney tissue. ICC/IF: HeLa cells. Flow: HeLa cells.
ConjugationUnconjugated
GradeAzide Free, Carrier Free, ExactAb™, High Performance, Validated
Product Description

Rabbit anti Human PDCD6/ALG-2 Antibody, Polyclonal, could be used for WB, IHC, ICC, IF, Flow and so on.
Application:
WB: 0.01-2 µg/mL
IHC: 5-20 µg/mL
ICC/IF: 5-20 µg/mL
Flow: 20 µg/mL
Protein Function:
Calcium-binding protein required for T-cell receptor-, Fas-, and glucocorticoid-induced cell death. May mediate Ca(2+)-regulated signals along the death pathway (By similarity). Calcium-dependent adapter necessary for the association between PDCD6IP and TSG101. Interaction with DAPK1 can accelerate apoptotic cell death by increasing caspase-3 activity. May inhibit KDR/VEGFR2-dependent angiogenesis; the function involves inhibition of VEGF-induced phosphoprylation of the Akt signaling pathway. Seems to play a role in the regulation of the distribution and function of MCOLN1 in the endosomal pathway. Isoform 2 has a lower Ca(2+) affinity than isoform 1. Isoform 1 and, to a lesser extend, isoform 2, can stabilize SHISA5.

Product Properties

IsotypeRabbit IgG
SDS-PAGE150 kDa
Purification MethodAntigen affinity purified + Protein A purified
FormLiquid
ConcentrationLot by Lot
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

PDCD6/ALG-2 Antibody (Ab120811) - Western Blot
All lanes: PDCD6/ALG-2 Antibody (Ab120811) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 30 kDa
Observed band size: 23 kDa
Exposure time: 6.0 s

PDCD6/ALG-2 Antibody (Ab120811) - IF/ICC
FITC staining on IF
Samples: Human HeLa cell
Primary Ab: 20 µg/mL PDCD6/ALG-2 Antibody (Ab120811)
Second Ab: 1 µg/mL FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

PDCD6/ALG-2 Antibody (Ab120811) - IHC
DAB staining on IHC
Samples: Human Kidney Tissue
Primary Ab: 20 μg/mL PDCD6/ALG-2 Antibody (Ab120811)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

PDCD6/ALG-2 Antibody (Ab120811) - Flow Cytometry
Human HeLa cell was fixed with 2% paraformaldehyde (10 min), permeabilized with 0.1% BSA-Triton X-100, then stained with 20 µg/mL PDCD6/ALG-2 Antibody (Ab120811) (red histogram) or Isotype control antibody (green histogram), followed by 1 µg/mL FITC-conjugated Anti-rabbit IgG Secondary Antibody.

Associated Targets(Human)

PDCD6 Tbio Programmed cell death protein 6 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Mechanisms of Action

Mechanism of ActionAction Typetarget IDTarget NameTarget TypeTarget OrganismBinding Site NameReferences

Application

ApplicationDilution info
IHC

5-20 µg/mL

WB

0.01-2 µg/mL

IF/ICC

5-20 µg/mL

Flow Cytometry

20μg/mL

Certificates

Certificate of Analysis(COA)

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Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate TypeDateItem
ZJ24F0404398Certificate of AnalysisApr 10, 2024 Ab120811
ZJ24F0404397Certificate of AnalysisApr 10, 2024 Ab120811
ZJ24F0404396Certificate of AnalysisApr 10, 2024 Ab120811

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