ExactAb™, Validated, Carrier Free, Azide Free, High performance, Lot by Lot
Host species
Rabbit
Specificity
PDCD6/ALG-2
Immunogen
Recombinant PDCD6 expressed in E.coli (AA 32-191)
Positive Control
WB: Hep G2 and Jurkat cell lysates. IHC: Human kidney tissue. ICC/IF: HeLa cells. Flow: HeLa cells.
Conjugation
Unconjugated
Grade
Azide Free, Carrier Free, ExactAb™, High Performance, Validated
Product Description
Rabbit anti Human PDCD6/ALG-2 Antibody, Polyclonal, could be used for WB, IHC, ICC, IF, Flow and so on. Application: WB: 0.01-2 µg/mL IHC: 5-20 µg/mL ICC/IF: 5-20 µg/mL Flow: 20 µg/mL Protein Function: Calcium-binding protein required for T-cell receptor-, Fas-, and glucocorticoid-induced cell death. May mediate Ca(2+)-regulated signals along the death pathway (By similarity). Calcium-dependent adapter necessary for the association between PDCD6IP and TSG101. Interaction with DAPK1 can accelerate apoptotic cell death by increasing caspase-3 activity. May inhibit KDR/VEGFR2-dependent angiogenesis; the function involves inhibition of VEGF-induced phosphoprylation of the Akt signaling pathway. Seems to play a role in the regulation of the distribution and function of MCOLN1 in the endosomal pathway. Isoform 2 has a lower Ca(2+) affinity than isoform 1. Isoform 1 and, to a lesser extend, isoform 2, can stabilize SHISA5.
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified + Protein A purified
Form
Liquid
Concentration
Lot by Lot
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
PDCD6/ALG-2 Antibody (Ab120811) - Western Blot All lanes: PDCD6/ALG-2 Antibody (Ab120811) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 30 kDa Observed band size: 23 kDa Exposure time: 6.0 s
PDCD6/ALG-2 Antibody (Ab120811) - IF/ICC FITC staining on IF Samples: Human HeLa cell Primary Ab: 20 µg/mL PDCD6/ALG-2 Antibody (Ab120811) Second Ab: 1 µg/mL FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PDCD6/ALG-2 Antibody (Ab120811) - IHC DAB staining on IHC Samples: Human Kidney Tissue Primary Ab: 20 μg/mL PDCD6/ALG-2 Antibody (Ab120811) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PDCD6/ALG-2 Antibody (Ab120811) - Flow Cytometry Human HeLa cell was fixed with 2% paraformaldehyde (10 min), permeabilized with 0.1% BSA-Triton X-100, then stained with 20 µg/mL PDCD6/ALG-2 Antibody (Ab120811) (red histogram) or Isotype control antibody (green histogram), followed by 1 µg/mL FITC-conjugated Anti-rabbit IgG Secondary Antibody.
Associated Targets(Human)
PDCD6TbioProgrammed cell death protein 6 (0 Activities)