PDK2 Antibody - Primary antibody, specific to PDK2, Rabbit IgG

    Application:
  • IHC
  • WB
Features and benefitsView More
  • Host species: Rabbit
  • Species reactivity(Reacts with): Human,Mouse,Rat
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
Item Number
Ab120993
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab120993-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$69.90
Ab120993-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$199.90
Ab120993-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$309.90
Ab120993-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,699.90

pAb; Rabbit anti Human PDK2 Antibody; WB, IHC; Unconjugated

Basic Description

Product NamePDK2 Antibody - Primary antibody, specific to PDK2, Rabbit IgG
SynonymsPDHK2 | Pyruvate dehydrogenase kinase isoform 2 | pyruvate dehydrogenase kinase, isozyme 2 | Pyruvate dehydrogenase lipoamide kinase isozyme 2, mitochondrial | PDK2_HUMAN | [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2 antibody | mitochondrial ant
Specifications & PurityValidated, 1.0 mg/mL
Host speciesRabbit
SpecificityPDK2
ImmunogenA synthetic peptide derived from human PDK2 (AA 260-309)
Positive ControlWB: Mouse brain, Rat heart lysates. IHC: Human liver cancer tissue.
ConjugationUnconjugated
GradeValidated
Product Description

Rabbit anti Human PDK2 Antibody, Polyclonal, could be used for WB, IHC and so on.
Application
WB: 1/500-1/2000
IHC: 1/100 - 1/300
Protein Function
Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.

Product Properties

IsotypeRabbit IgG
SDS-PAGE150 kDa
Purification MethodImmunogen affinity purified
FormLiquid
Concentration1.0 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20℃ long term (12 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

PDK2 Antibody (Ab120993) - Western Blot
All lanes: PDK2 Antibody (Ab120993) at 1/500 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution

Predicted band size: 46 kDa
Observed band size: 46 kDa
Exposure time: 100.0 s

PDK2 Antibody (Ab120993) - IHC
Immunohistochemical analysis of paraffin-embedded human liver cancer. PDK2 Antibody (Ab120993) was diluted at 1/200 (4° overnight). Tris-EDTA, pH 9.0 was used for antigen retrieval. Secondary antibody was diluted at 1/200 (room temperature, 45 min). 

Associated Targets(Human)

PDK2 Tchem [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
PDK2 Tchem Pyruvate dehydrogenase kinase isoform 2 (894 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID

Mechanisms of Action

Mechanism of ActionAction Typetarget IDTarget NameTarget TypeTarget OrganismBinding Site NameReferences
Pyruvate dehydrogenase kinase inhibitorINHIBITORALA2096665Pyruvate dehydrogenase kinasePROTEIN FAMILYHomo sapiens

PubMed: [1] , [2]


Application

ApplicationDilution info
WB1/500-1/2000

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1/500-1/2000

IHC1/100-1/300

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1/100-1/300

Certificates

C of A & Other Certificates

Related Documents

Solution Calculators