pAb; Rabbit anti Human Pentraxin 3/PTX3 Antibody; WB, IHC, ICC, IF; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab121192-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $89.90 | |
| Ab121192-50μl | 50μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $269.90 | |
| Ab121192-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $379.90 | |
| Ab121192-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,899.90 |
pAb; Rabbit anti Human Pentraxin 3/PTX3 Antibody; WB, IHC, ICC, IF; Unconjugated
| Product Name | Pentraxin 3/PTX3 Antibody |
|---|---|
| Specifications & Purity | ExactAb™, Validated, Carrier Free, 1.0 mg/mL |
| Host species | Rabbit |
| Specificity | Pentraxin 3/PTX3 |
| Immunogen | A synthetic peptide derived from human PTX3 |
| Positive Control | WB: Mouse spleen, HEK293, A549, HUVEC, NIH/3T3 and C6 lysates. IHC: Human lung cancer tissue. ICC/IF: HUVEC cells. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description |
Rabbit anti Human Pentraxin 3/PTX3 Antibody, Polyclonal, could be used for WB, IHC, ICC, IF and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Immunogen affinity purified |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Pentraxin 3/PTX3 Antibody (Ab121192) - Western Blot
All lanes: Pentraxin 3/PTX3 Antibody (Ab121192) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 41 kDa
Observed band size: 44 kDa
Exposure time: 12.9 s
Pentraxin 3/PTX3 Antibody (Ab121192) - IF/ICC
Immunofluorescent analysis of PTX3 staining in HuvEc cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the Pentraxin 3/PTX3 Antibody (Ab121192) in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Pentraxin 3/PTX3 Antibody (Ab121192) - IHC
Immunohistochemical analysis of PTX3 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the Pentraxin 3/PTX3 Antibody (Ab121192) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| ICC/IF |
1/50-1/200
|
| IHC | 1/50 - 1/100 |
| WB |
1/500-1/1000
|
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