POLR2I Antibody

    Application:
  • WB
Features and benefits
  • Host species: Rabbit
  • Species reactivity(Reacts with): Chicken,Cow,Dog,Human,Monkey,Mouse,Pig,Rabbit,Rat
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
Item Number
Ab122597
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab122597-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$79.90
Ab122597-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$219.90
Ab122597-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$319.90
Ab122597-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,099.90

pAb; Rabbit anti Human POLR2I Antibody; WB; Unconjugated

Basic Description

Product NamePOLR2I Antibody
SynonymsDNA directed RNA polymerase II polypeptide I antibody | DNA directed RNA polymerase II subunit I antibody | DNA directed RNA polymerase II subunit RPB9 antibody | DNA-directed RNA polymerase II subunit I antibody | DNA-directed RNA polymerase II subunit R
Specifications & PurityExactAb™, Validated, Carrier Free, 1.0 mg/mL
Host speciesRabbit
ImmunogenA KLH-conjugated synthetic peptide encompassing a sequence within the center region of human POLR2I.
Positive ControlWB: A549, Hep G2, MCF7, and C6 cell lysates.
ConjugationUnconjugated
GradeCarrier Free, ExactAb™, Validated
Product Description

Rabbit anti Human POLR2I Antibody, Polyclonal, could be used for WB and so on.
Application:
WB: 1/500-1/1000
Protein Function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB9 is part of the upper jaw surrounding the central large cleft and thought to grab the incoming DNA template.
Post-translational
Phosphorylated upon DNA damage, probably by ATM or ATR.

Product Properties

IsotypeRabbit IgG
SDS-PAGE150 kDa
Purification MethodImmunogen affinity purified
FormLiquid
Concentration1.0 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

POLR2I Antibody (Ab122597) - Western Blot
All lanes: POLR2I Antibody (Ab122597) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 14 kDa
Observed band size: 15 kDa
Exposure time: 200.0 s

POLR2I Antibody (Ab122597) - Western Blot
All lanes: POLR2I Antibody (Ab122597) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 14 kDa
Observed band size: 15 kDa
Exposure time: 30.0 s 

Associated Targets(Human)

POLR2I Tbio DNA-directed RNA polymerase II subunit RPB9 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
WB1/500-1/1000

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1/500-1/1000

Certificates

Certificate of Analysis(COA)

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Related Documents

Solution Calculators