| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab123227-10μl | 10μl | In stock | $69.90 | |
| Ab123227-50μl | 50μl | In stock | $189.90 | |
| Ab123227-100μl | 100μl | In stock | $289.90 | |
| Ab123227-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $1,999.90 |
pAb; Rabbit anti Human Prohibitin Antibody; WB, IHC; Unconjugated
| Product Name | Prohibitin Antibody - Primary antibody, specific to Prohibitin, Rabbit IgG |
|---|---|
| Synonyms | Epididymis luminal protein 215 antibody | Epididymis secretory sperm binding protein Li 54e antibody | HEL 215 antibody | HEL S 54e antibody | PHB antibody | PHB_HUMAN antibody | PHB1 antibody | Prohibitin antibody | PHB | PHB1 | Prohibitin | HEL-215 | HE |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Azide Free, High performance, Lot by Lot |
| Host species | Rabbit |
| Specificity | Prohibitin |
| Immunogen | Recombinant PHB expressed in E.coli (AA 177-272). |
| Positive Control | WB: HeLa, MCF7, Ramos, A431, HEK293, Hep G2, Jurkat, A549, NIH/3T3 and C6 cell lysates. IHC: Human breast cancer, rectum, thyroid cancer, liver, liver cancer tissues; Mouse cardiac muscle tissue. |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, High Performance, Validated |
| Product Description | Rabbit anti Human Prohibitin Antibody, Polyclonal, could be used for WB, IHC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified + Protein A purified |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Prohibitin Antibody (Ab123227) - Western Blot
All lanes: Prohibitin Antibody (Ab123227) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 30 kDa
Observed band size: 28 kDa
Exposure time: 14.8 s
Prohibitin Antibody (Ab123227) - IHC
DAB staining on IHC
Samples: Human Breast cancer Tissue
Primary Ab: 10 µg/mL Prohibitin Antibody (Ab123227)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Prohibitin Antibody (Ab123227) - IHC
DAB staining on IHC
Samples: Human Rectum Tissue
Primary Ab: 10 µg/mL Prohibitin Antibody (Ab123227)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Prohibitin Antibody (Ab123227) - IHC
DAB staining on IHC
Samples: Human Thyroid cancer Tissue
Primary Ab: 10 µg/mL Prohibitin Antibody (Ab123227)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Prohibitin Antibody (Ab123227) - IHC
DAB staining on IHC
Samples: Human Liver Tissue
Primary Ab: 10 µg/mL Prohibitin Antibody (Ab123227)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Prohibitin Antibody (Ab123227) - IHC
DAB staining on IHC
Samples: Human Liver cancer Tissue
Primary Ab: 10 µg/mL Prohibitin Antibody (Ab123227)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Prohibitin Antibody (Ab123227) - IHC
DAB staining on IHC
Sample: Mouse Cardiac Muscle Tissue
Primary Ab: 30 µg/mL Prohibitin Antibody (Ab123227)
Control: Used PBS instead of primary antibody
Second Ab: 2 μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
| Application | Dilution info |
|---|---|
| IHC | 5-30 µg/mL |
| WB |
0.01-2 µg/mL
|
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0404501 | Certificate of Analysis | Apr 15, 2024 | Ab123227 |
| ZJ24F0404500 | Certificate of Analysis | Apr 15, 2024 | Ab123227 |
| ZJ24F0404499 | Certificate of Analysis | Apr 15, 2024 | Ab123227 |
| 1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M. (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.. Science, 325 (5942): (834-40). [PMID:19608861] [10.1021/op500134e] |
| 2. Cornillez-Ty CT, Liao L, Yates 3rd JR, Kuhn P, Buchmeier MJ. (2009) Severe acute respiratory syndrome coronavirus nonstructural protein 2 interacts with a host protein complex involved in mitochondrial biogenesis and intracellular signaling.. J Virol, 83 (19): (10314-8). [PMID:19640993] [10.1021/op500134e] |
| 3. Djehal A, Krayem M, Najem A, Hammoud H, Cresteil T, Nebigil CG, Wang D, Yu P, Bentouhami E, Ghanem GE, Désaubry L.. (2018) Targeting prohibitin with small molecules to promote melanogenesis and apoptosis in melanoma cells.. Eur J Med Chem, 155 (880-888). [PMID:29960207] [10.1016/j.ejmech.2018.06.052] |
| 4. Sato, T T and 9 more authors.. (1992) The human prohibitin gene located on chromosome 17q21 is mutated in sporadic breast cancer.. Cancer research, (15): [PMID:1540973] |
| 5. Sato, T T and 5 more authors.. (1993) The human prohibitin (PHB) gene family and its somatic mutations in human tumors.. Genomics, [PMID:8244394] |
| 6. Coates, P J PJ and 6 more authors.. (2001) Mammalian prohibitin proteins respond to mitochondrial stress and decrease during cellular senescence.. Experimental cell research, (1): [PMID:11302691] |
| 7. Fusaro, Gina G, Dasgupta, Piyali P, Rastogi, Shipra S, Joshi, Bharat B and Chellappan, Srikumar S.. (2003) Prohibitin induces the transcriptional activity of p53 and is exported from the nucleus upon apoptotic signaling.. The Journal of biological chemistry, (28): [PMID:14500729] |
| 8. Zody, Michael C MC and 73 more authors.. (2006) DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage.. Nature, (20): [PMID:16625196] |
| 9. Emerson, Vanessa V and 6 more authors.. (2010) Identification of the cellular prohibitin 1/prohibitin 2 heterodimer as an interaction partner of the C-terminal cytoplasmic domain of the HIV-1 glycoprotein.. Journal of virology, [PMID:19906925] |
| 10. Christie, Darah A DA and 9 more authors.. (2011) Stomatin-like protein 2 binds cardiolipin and regulates mitochondrial biogenesis and function.. Molecular and cellular biology, [PMID:21746876] |
| 11. Wintachai, Phitchayapak P and 13 more authors.. (2012) Identification of prohibitin as a Chikungunya virus receptor protein.. Journal of medical virology, [PMID:22997079] |
| 12. Fu, Ping P, Yang, Zhiyong Z and Bach, Leon A LA.. (2013) Prohibitin-2 binding modulates insulin-like growth factor-binding protein-6 (IGFBP-6)-induced rhabdomyosarcoma cell migration.. The Journal of biological chemistry, (11): [PMID:24003225] |
| 13. Wei, Yongjie Y, Chiang, Wei-Chung WC, Sumpter, Rhea R, Mishra, Prashant P and Levine, Beth B.. (2017) Prohibitin 2 Is an Inner Mitochondrial Membrane Mitophagy Receptor.. Cell, (12): [PMID:28017329] |
| 14. Yoshinaka, Takahiro and 7 more authors.. (2019) Structural Basis of Mitochondrial Scaffolds by Prohibitin Complexes: Insight into a Role of the Coiled-Coil Region.. iScience, (27): [PMID:31522117] |
| 15. Zhang, Junling; Sun, Zian; Wu, Qingsi and Shen, Jijia.. (2020) Prohibitin 1 interacts with signal transducer and activator of transcription 3 in T-helper 17 cells.. Immunology letters, [PMID:31899195] |
