Intercalating cationic dye with specificity for RNA.
Basic Description
Synonyms
Pyronin Y | 92-32-0 | Pyronine Y | Pyronine | Pyronin G | Pyronin Yellow | Pyronine G | C.I. 45005 | Xanthylium, 3,6-bis(dimethylamino)-, chloride | 3,6-Bis(dimethylamino)xanthylium chloride | Schultz No. 853 | C17H19ClN2O | CHEBI:8682 | AMMONIUM, (6-(DIMETHYLAMINO)-3H-XANTEN-3-YL
Specifications & Purity
Biological stain
Biochemical and Physiological Mechanisms
Cationic dye that intercalates RNA and is visible as a red band during electrophoresis. Shown to accumulate in the mitochondria of viable cells. At low concentrations the cytostatic effect is cell arrest in the G1 phase
Storage Temp
Store at -20°C
Shipped In
Ice chest + Ice pads
Grade
Biological Stain
Note
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
Product Description
Pyronin Y is a dye that intercalates and stains RNA red, and has been shown to accumulate in the mitochondria of viable cells. Multidrug-resistant (MDR) Acinetobacter baumannii studies tested inhibition of the efflux pump by measuring the intracellluar accumulation of Pyronin Y and Ethidium bromide. Research shows that at low Pyronin Y concentrations the cytostatic effect is cell arrest in the G1 phase, whereas at high concentrations the cells arrest is in the G2 and S phase. Pyronin Y has been used in combination with Alcian Blue to asses and study pulmonary diseases, and in combination with Methyl Green to stain nucleic acids in paraffin sections. Additionally Pyronin Y has been used to measure RNA in flow cytometry. Used in Unna-Pappenheim stain to demonstrate RNA (red) in contrast to DNA (green). Also used for bacteria staining.
1.Kara E et al.. (2021) An integrated genomic approach to dissect the genetic landscape regulating the cell-to-cell transfer of a-synuclein.. Cell Rep, 35 (10):(109189). [PMID:34107263]
2.Rahmé R. (2021) Assaying Cell Cycle Status Using Flow Cytometry.. Methods Mol Biol, 2267 (165-179). [PMID:33786791]
3.Shaban HA et al.. (2020) Hi-D: nanoscale mapping of nuclear dynamics in single living cells.. Genome Biol, 21 (95). [PMID:32312289]
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