Rad9 Mouse mAb - Primary antibody, specific to Rad9, Mouse IgG2b

Features and benefits
  • Short Overview:

    mAb (3A3-A7-F8); Mouse anti Human Rad9 Antibody; WB, IP; Unconjugated

  • Species reactivity(Reacts with): Human,Monkey,Mouse,Rat
  • Isotype: Mouse IgG2b
    Application:
  • IP
  • WB
Item Number
Ab124408
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab124408-10μl
10μl
In stock
$69.90
Ab124408-50μl
50μl
In stock
$189.90
Ab124408-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$299.90
Ab124408-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,599.90

mAb (3A3-A7-F8); Mouse anti Human Rad9 Antibody; WB, IP; Unconjugated

Basic Description

Product NameRad9 Mouse mAb - Primary antibody, specific to Rad9, Mouse IgG2b
SynonymsDNA repair exonuclease rad9 homolog A | EC 3.1.11.2 | hRAD9 | Cell cycle checkpoint control protein antibody | Cell cycle checkpoint control protein RAD9A antibody | DNA repair exonuclease rad9 homolog A antibody | hRAD 9 antibody | hRAD9 antibody | Rad 9
Specifications & PurityExactAb™, Validated, 1.0 mg/mL
Host speciesMouse
SpecificityRad9
ImmunogenA synthetic peptide derived from human RAD9A (AA 264-370)
Positive ControlWB: HeLa, MCF7, NIH/3T3 and C6 cell lysates. IP: HeLa and NIH/3T3 cell lysates.
ConjugationUnconjugated
GradeExactAb™, Validated
Product Description

Mouse anti Human Rad9 Antibody, Monoclonal (3A3-A7-F8), could be used for WB, IP and so on.
Application:
WB: 1/500-1/1000
IP: 1/20
Protein Function:
Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. RAD9A possesses 3'->5' double stranded DNA exonuclease activity. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.

Product Properties

IsotypeMouse IgG2b
Light Chain Typekappa
SDS-PAGE150 kDa
Purification MethodProtein G purified
FormLiquid
Concentration1.0 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Rad9 Mouse mAb (Ab124408) - Western Blot
All lanes: Rad9 Mouse mAb (Ab124408) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 43 kDa
Observed band size: 49 kDa
Exposure time: 16.2 s

Rad9 Mouse mAb (Ab124408) - Western Blot
All lanes: Rad9 Mouse mAb (Ab124408) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 43 kDa
Observed band size: 48 kDa
Exposure time: 20.0 s

Rad9 Mouse mAb (Ab124408) - IP
Immunoprecipitation analysis of Rad9 in HeLa and NIH/3T3 lysates using Rad9 Mouse mAb (Ab124408).

Associated Targets(Human)

RAD9A Tbio Cell cycle checkpoint control protein RAD9A (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
WB

1/500-1/1000

IP

1/20

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0505942Certificate of AnalysisMay 22, 2024 Ab124408
ZJ24F0505943Certificate of AnalysisMay 22, 2024 Ab124408

Related Documents

References

1. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed.  (2009-05-06)  Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach..  Analytical chemistry,  81  ((11)): (4493-4501).  [PMID:19413330]
2. Lieberman, H B HB, Hopkins, K M KM, Nass, M M, Demetrick, D D and Davey, S S..  (1996)  A human homolog of the Schizosaccharomyces pombe rad9+ checkpoint control gene..  Proceedings of the National Academy of Sciences of the United States of America,    (26):   [PMID:8943031]
3. St Onge, R P RP, Udell, C M CM, Casselman, R R and Davey, S S..  (1999)  The human G2 checkpoint control protein hRAD9 is a nuclear phosphoprotein that forms complexes with hRAD1 and hHUS1..  Molecular biology of the cell,      [PMID:10359610]
4. Bessho, T T and Sancar, A A..  (2000)  Human DNA damage checkpoint protein hRAD9 is a 3' to 5' exonuclease..  The Journal of biological chemistry,    (17):   [PMID:10713044]
5. Hang, H H and Lieberman, H B HB..  (2000)  Physical interactions among human checkpoint control proteins HUS1p, RAD1p, and RAD9p, and implications for the regulation of cell cycle progression..  Genomics,    (1):   [PMID:10777662]
6. Cai, R L RL, Yan-Neale, Y Y, Cueto, M A MA, Xu, H H and Cohen, D D..  (2000)  HDAC1, a histone deacetylase, forms a complex with Hus1 and Rad9, two G2/M checkpoint Rad proteins..  The Journal of biological chemistry,    (8):   [PMID:10846170]
7. Rauen, M M, Burtelow, M A MA, Dufault, V M VM and Karnitz, L M LM..  (2000)  The human checkpoint protein hRad17 interacts with the PCNA-like proteins hRad1, hHus1, and hRad9..  The Journal of biological chemistry,    (22):   [PMID:10884395]
8. Xiang, S L SL and 5 more authors..  (2001)  The J domain of Tpr2 regulates its interaction with the proapoptotic and cell-cycle checkpoint protein, Rad9..  Biochemical and biophysical research communications,    (5):   [PMID:11573955]
9. Yoshida, Kiyotsugu K, Komatsu, Kiyoshi K, Wang, Hong-Gang HG and Kufe, Donald D..  (2002)  c-Abl tyrosine kinase regulates the human Rad9 checkpoint protein in response to DNA damage..  Molecular and cellular biology,      [PMID:11971963]
10. Bermudez, Vladimir P VP and 6 more authors..  (2003)  Loading of the human 9-1-1 checkpoint complex onto DNA by the checkpoint clamp loader hRad17-replication factor C complex in vitro..  Proceedings of the National Academy of Sciences of the United States of America,    (18):   [PMID:12578958]
11. Yoshida, Kiyotsugu K, Wang, Hong-Gang HG, Miki, Yoshio Y and Kufe, Donald D..  (2003)  Protein kinase Cdelta is responsible for constitutive and DNA damage-induced phosphorylation of Rad9..  The EMBO journal,    (17):   [PMID:12628935]
12. Roos-Mattjus, Pia P and 7 more authors..  (2003)  Phosphorylation of human Rad9 is required for genotoxin-activated checkpoint signaling..  The Journal of biological chemistry,    (4):   [PMID:12709442]
13. Hopkins, Kevin M KM and 5 more authors..  (2003)  Expression of mammalian paralogues of HRAD9 and Mrad9 checkpoint control genes in normal and cancerous testicular tissue..  Cancer research,    (1):   [PMID:14500360]
14. Toueille, Magali M and 9 more authors..  (2004)  The human Rad9/Rad1/Hus1 damage sensor clamp interacts with DNA polymerase beta and increases its DNA substrate utilisation efficiency: implications for DNA repair..  Nucleic acids research,      [PMID:15314187]
15. Wang, Wensheng W and 7 more authors..  (2004)  The human Rad9-Rad1-Hus1 checkpoint complex stimulates flap endonuclease 1..  Proceedings of the National Academy of Sciences of the United States of America,    (30):   [PMID:15556996]
16. Smirnova, Ekaterina E, Toueille, Magali M, Markkanen, Enni E and Hübscher, Ulrich U..  (2005)  The human checkpoint sensor and alternative DNA clamp Rad9-Rad1-Hus1 modulates the activity of DNA ligase I, a component of the long-patch base excision repair machinery..  The Biochemical journal,    (1):   [PMID:15871698]
17. Wu, Xiaoming X, Shell, Steven M SM and Zou, Yue Y..  (2005)  Interaction and colocalization of Rad9/Rad1/Hus1 checkpoint complex with replication protein A in human cells..  Oncogene,    (7):   [PMID:15897895]
18. Friedrich-Heineken, Erica E and 6 more authors..  (2005)  The two DNA clamps Rad9/Rad1/Hus1 complex and proliferating cell nuclear antigen differentially regulate flap endonuclease 1 activity..  Journal of molecular biology,    (11):   [PMID:16216273]
19. Mayya, Viveka V and 7 more authors..  (2009)  Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions..  Science signaling,    (18):   [PMID:19690332]
20. Rigbolt, Kristoffer T G KT and 9 more authors..  (2011)  System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation..  Science signaling,    (15):   [PMID:21406692]
21. Cotta-Ramusino, Cecilia C and 5 more authors..  (2011)  A DNA damage response screen identifies RHINO, a 9-1-1 and TopBP1 interacting protein required for ATR signaling..  Science (New York, N.Y.),    (10):   [PMID:21659603]

Solution Calculators