Basic Description

Product Name	Rad9 Mouse mAb - Primary antibody, specific to Rad9, Mouse IgG2b
Synonyms	DNA repair exonuclease rad9 homolog A \| EC 3.1.11.2 \| hRAD9 \| Cell cycle checkpoint control protein antibody \| Cell cycle checkpoint control protein RAD9A antibody \| DNA repair exonuclease rad9 homolog A antibody \| hRAD 9 antibody \| hRAD9 antibody \| Rad 9
Specifications & Purity	ExactAb™, Validated, 1.0 mg/mL
Host species	Mouse
Specificity	Rad9
Immunogen	A synthetic peptide derived from human RAD9A (AA 264-370)
Positive Control	WB: HeLa, MCF7, NIH/3T3 and C6 cell lysates. IP: HeLa and NIH/3T3 cell lysates.
Conjugation	Unconjugated
Grade	ExactAb™, Validated
Product Description	Mouse anti Human Rad9 Antibody, Monoclonal (3A3-A7-F8), could be used for WB, IP and so on. Application: WB: 1/500-1/1000 IP: 1/20 Protein Function: Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. RAD9A possesses 3'->5' double stranded DNA exonuclease activity. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.

Product Properties

Isotype	Mouse IgG2b
Light Chain Type	kappa
SDS-PAGE	150 kDa
Purification Method	Protein G purified
Form	Liquid
Concentration	1.0 mg/mL
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Rad9 Mouse mAb (Ab124408) - Western Blot
All lanes: Rad9 Mouse mAb (Ab124408) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 43 kDa
Observed band size: 49 kDa
Exposure time: 16.2 s

Rad9 Mouse mAb (Ab124408) - Western Blot
All lanes: Rad9 Mouse mAb (Ab124408) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 43 kDa
Observed band size: 48 kDa
Exposure time: 20.0 s

Rad9 Mouse mAb (Ab124408) - IP
Immunoprecipitation analysis of Rad9 in HeLa and NIH/3T3 lysates using Rad9 Mouse mAb (Ab124408).

Associated Targets

RAD9A Tbio Cell cycle checkpoint control protein RAD9A 0 Activities

Discover Target: RAD9A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Application

Application	Dilution info
WB	1/500-1/1000
IP	1/20

Certificates

Certificate of Analysis(COA)

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2 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0505942	Certificate of Analysis	May 22, 2024	Ab124408
ZJ24F0505943	Certificate of Analysis	May 22, 2024	Ab124408

FAQs and Articles

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Guidelines of Primary Antibodies for Western Blotting

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References

1. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330]
2. Lieberman, H B HB, Hopkins, K M KM, Nass, M M, Demetrick, D D and Davey, S S.. (1996) A human homolog of the Schizosaccharomyces pombe rad9+ checkpoint control gene.. Proceedings of the National Academy of Sciences of the United States of America, (26): [PMID:8943031]
3. St Onge, R P RP, Udell, C M CM, Casselman, R R and Davey, S S.. (1999) The human G2 checkpoint control protein hRAD9 is a nuclear phosphoprotein that forms complexes with hRAD1 and hHUS1.. Molecular biology of the cell, [PMID:10359610]
4. Bessho, T T and Sancar, A A.. (2000) Human DNA damage checkpoint protein hRAD9 is a 3' to 5' exonuclease.. The Journal of biological chemistry, (17): [PMID:10713044]
5. Hang, H H and Lieberman, H B HB.. (2000) Physical interactions among human checkpoint control proteins HUS1p, RAD1p, and RAD9p, and implications for the regulation of cell cycle progression.. Genomics, (1): [PMID:10777662]
6. Cai, R L RL, Yan-Neale, Y Y, Cueto, M A MA, Xu, H H and Cohen, D D.. (2000) HDAC1, a histone deacetylase, forms a complex with Hus1 and Rad9, two G2/M checkpoint Rad proteins.. The Journal of biological chemistry, (8): [PMID:10846170]
7. Rauen, M M, Burtelow, M A MA, Dufault, V M VM and Karnitz, L M LM.. (2000) The human checkpoint protein hRad17 interacts with the PCNA-like proteins hRad1, hHus1, and hRad9.. The Journal of biological chemistry, (22): [PMID:10884395]
8. Xiang, S L SL and 5 more authors.. (2001) The J domain of Tpr2 regulates its interaction with the proapoptotic and cell-cycle checkpoint protein, Rad9.. Biochemical and biophysical research communications, (5): [PMID:11573955]
9. Yoshida, Kiyotsugu K, Komatsu, Kiyoshi K, Wang, Hong-Gang HG and Kufe, Donald D.. (2002) c-Abl tyrosine kinase regulates the human Rad9 checkpoint protein in response to DNA damage.. Molecular and cellular biology, [PMID:11971963]
10. Bermudez, Vladimir P VP and 6 more authors.. (2003) Loading of the human 9-1-1 checkpoint complex onto DNA by the checkpoint clamp loader hRad17-replication factor C complex in vitro.. Proceedings of the National Academy of Sciences of the United States of America, (18): [PMID:12578958]
11. Yoshida, Kiyotsugu K, Wang, Hong-Gang HG, Miki, Yoshio Y and Kufe, Donald D.. (2003) Protein kinase Cdelta is responsible for constitutive and DNA damage-induced phosphorylation of Rad9.. The EMBO journal, (17): [PMID:12628935]
12. Roos-Mattjus, Pia P and 7 more authors.. (2003) Phosphorylation of human Rad9 is required for genotoxin-activated checkpoint signaling.. The Journal of biological chemistry, (4): [PMID:12709442]
13. Hopkins, Kevin M KM and 5 more authors.. (2003) Expression of mammalian paralogues of HRAD9 and Mrad9 checkpoint control genes in normal and cancerous testicular tissue.. Cancer research, (1): [PMID:14500360]
14. Toueille, Magali M and 9 more authors.. (2004) The human Rad9/Rad1/Hus1 damage sensor clamp interacts with DNA polymerase beta and increases its DNA substrate utilisation efficiency: implications for DNA repair.. Nucleic acids research, [PMID:15314187]
15. Wang, Wensheng W and 7 more authors.. (2004) The human Rad9-Rad1-Hus1 checkpoint complex stimulates flap endonuclease 1.. Proceedings of the National Academy of Sciences of the United States of America, (30): [PMID:15556996]
16. Smirnova, Ekaterina E, Toueille, Magali M, Markkanen, Enni E and Hübscher, Ulrich U.. (2005) The human checkpoint sensor and alternative DNA clamp Rad9-Rad1-Hus1 modulates the activity of DNA ligase I, a component of the long-patch base excision repair machinery.. The Biochemical journal, (1): [PMID:15871698]
17. Wu, Xiaoming X, Shell, Steven M SM and Zou, Yue Y.. (2005) Interaction and colocalization of Rad9/Rad1/Hus1 checkpoint complex with replication protein A in human cells.. Oncogene, (7): [PMID:15897895]
18. Friedrich-Heineken, Erica E and 6 more authors.. (2005) The two DNA clamps Rad9/Rad1/Hus1 complex and proliferating cell nuclear antigen differentially regulate flap endonuclease 1 activity.. Journal of molecular biology, (11): [PMID:16216273]
19. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332]
20. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692]
21. Cotta-Ramusino, Cecilia C and 5 more authors.. (2011) A DNA damage response screen identifies RHINO, a 9-1-1 and TopBP1 interacting protein required for ATR signaling.. Science (New York, N.Y.), (10): [PMID:21659603]

Solution Calculators

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SKU	Size	Availability	Price
Ab124408-10μl	10μl	In stock	$69.90
Ab124408-50μl	50μl	In stock	$189.90
Ab124408-100μl	100μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$299.90
Ab124408-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$2,599.90

Rad9 Mouse mAb - Primary antibody, specific to Rad9, Mouse IgG2b