| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab124408-10μl | 10μl | In stock | $69.90 | |
| Ab124408-50μl | 50μl | In stock | $189.90 | |
| Ab124408-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| Ab124408-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,599.90 |
mAb (3A3-A7-F8); Mouse anti Human Rad9 Antibody; WB, IP; Unconjugated
| Product Name | Rad9 Mouse mAb - Primary antibody, specific to Rad9, Mouse IgG2b |
|---|---|
| Synonyms | DNA repair exonuclease rad9 homolog A | EC 3.1.11.2 | hRAD9 | Cell cycle checkpoint control protein antibody | Cell cycle checkpoint control protein RAD9A antibody | DNA repair exonuclease rad9 homolog A antibody | hRAD 9 antibody | hRAD9 antibody | Rad 9 |
| Specifications & Purity | ExactAb™, Validated, 1.0 mg/mL |
| Host species | Mouse |
| Specificity | Rad9 |
| Immunogen | A synthetic peptide derived from human RAD9A (AA 264-370) |
| Positive Control | WB: HeLa, MCF7, NIH/3T3 and C6 cell lysates. IP: HeLa and NIH/3T3 cell lysates. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Validated |
| Product Description | Mouse anti Human Rad9 Antibody, Monoclonal (3A3-A7-F8), could be used for WB, IP and so on. |
| Isotype | Mouse IgG2b |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein G purified |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Rad9 Mouse mAb (Ab124408) - Western Blot
All lanes: Rad9 Mouse mAb (Ab124408) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 43 kDa
Observed band size: 49 kDa
Exposure time: 16.2 s
Rad9 Mouse mAb (Ab124408) - Western Blot
All lanes: Rad9 Mouse mAb (Ab124408) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 43 kDa
Observed band size: 48 kDa
Exposure time: 20.0 s
Rad9 Mouse mAb (Ab124408) - IP
Immunoprecipitation analysis of Rad9 in HeLa and NIH/3T3 lysates using Rad9 Mouse mAb (Ab124408).
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000"> 1/500-1/1000 |
| IP | 1/20 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0505942 | Certificate of Analysis | May 22, 2024 | Ab124408 |
| ZJ24F0505943 | Certificate of Analysis | May 22, 2024 | Ab124408 |
| 1. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330] |
| 2. Lieberman, H B HB, Hopkins, K M KM, Nass, M M, Demetrick, D D and Davey, S S.. (1996) A human homolog of the Schizosaccharomyces pombe rad9+ checkpoint control gene.. Proceedings of the National Academy of Sciences of the United States of America, (26): [PMID:8943031] |
| 3. St Onge, R P RP, Udell, C M CM, Casselman, R R and Davey, S S.. (1999) The human G2 checkpoint control protein hRAD9 is a nuclear phosphoprotein that forms complexes with hRAD1 and hHUS1.. Molecular biology of the cell, [PMID:10359610] |
| 4. Bessho, T T and Sancar, A A.. (2000) Human DNA damage checkpoint protein hRAD9 is a 3' to 5' exonuclease.. The Journal of biological chemistry, (17): [PMID:10713044] |
| 5. Hang, H H and Lieberman, H B HB.. (2000) Physical interactions among human checkpoint control proteins HUS1p, RAD1p, and RAD9p, and implications for the regulation of cell cycle progression.. Genomics, (1): [PMID:10777662] |
| 6. Cai, R L RL, Yan-Neale, Y Y, Cueto, M A MA, Xu, H H and Cohen, D D.. (2000) HDAC1, a histone deacetylase, forms a complex with Hus1 and Rad9, two G2/M checkpoint Rad proteins.. The Journal of biological chemistry, (8): [PMID:10846170] |
| 7. Rauen, M M, Burtelow, M A MA, Dufault, V M VM and Karnitz, L M LM.. (2000) The human checkpoint protein hRad17 interacts with the PCNA-like proteins hRad1, hHus1, and hRad9.. The Journal of biological chemistry, (22): [PMID:10884395] |
| 8. Xiang, S L SL and 5 more authors.. (2001) The J domain of Tpr2 regulates its interaction with the proapoptotic and cell-cycle checkpoint protein, Rad9.. Biochemical and biophysical research communications, (5): [PMID:11573955] |
| 9. Yoshida, Kiyotsugu K, Komatsu, Kiyoshi K, Wang, Hong-Gang HG and Kufe, Donald D.. (2002) c-Abl tyrosine kinase regulates the human Rad9 checkpoint protein in response to DNA damage.. Molecular and cellular biology, [PMID:11971963] |
| 10. Bermudez, Vladimir P VP and 6 more authors.. (2003) Loading of the human 9-1-1 checkpoint complex onto DNA by the checkpoint clamp loader hRad17-replication factor C complex in vitro.. Proceedings of the National Academy of Sciences of the United States of America, (18): [PMID:12578958] |
| 11. Yoshida, Kiyotsugu K, Wang, Hong-Gang HG, Miki, Yoshio Y and Kufe, Donald D.. (2003) Protein kinase Cdelta is responsible for constitutive and DNA damage-induced phosphorylation of Rad9.. The EMBO journal, (17): [PMID:12628935] |
| 12. Roos-Mattjus, Pia P and 7 more authors.. (2003) Phosphorylation of human Rad9 is required for genotoxin-activated checkpoint signaling.. The Journal of biological chemistry, (4): [PMID:12709442] |
| 13. Hopkins, Kevin M KM and 5 more authors.. (2003) Expression of mammalian paralogues of HRAD9 and Mrad9 checkpoint control genes in normal and cancerous testicular tissue.. Cancer research, (1): [PMID:14500360] |
| 14. Toueille, Magali M and 9 more authors.. (2004) The human Rad9/Rad1/Hus1 damage sensor clamp interacts with DNA polymerase beta and increases its DNA substrate utilisation efficiency: implications for DNA repair.. Nucleic acids research, [PMID:15314187] |
| 15. Wang, Wensheng W and 7 more authors.. (2004) The human Rad9-Rad1-Hus1 checkpoint complex stimulates flap endonuclease 1.. Proceedings of the National Academy of Sciences of the United States of America, (30): [PMID:15556996] |
| 16. Smirnova, Ekaterina E, Toueille, Magali M, Markkanen, Enni E and Hübscher, Ulrich U.. (2005) The human checkpoint sensor and alternative DNA clamp Rad9-Rad1-Hus1 modulates the activity of DNA ligase I, a component of the long-patch base excision repair machinery.. The Biochemical journal, (1): [PMID:15871698] |
| 17. Wu, Xiaoming X, Shell, Steven M SM and Zou, Yue Y.. (2005) Interaction and colocalization of Rad9/Rad1/Hus1 checkpoint complex with replication protein A in human cells.. Oncogene, (7): [PMID:15897895] |
| 18. Friedrich-Heineken, Erica E and 6 more authors.. (2005) The two DNA clamps Rad9/Rad1/Hus1 complex and proliferating cell nuclear antigen differentially regulate flap endonuclease 1 activity.. Journal of molecular biology, (11): [PMID:16216273] |
| 19. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332] |
| 20. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692] |
| 21. Cotta-Ramusino, Cecilia C and 5 more authors.. (2011) A DNA damage response screen identifies RHINO, a 9-1-1 and TopBP1 interacting protein required for ATR signaling.. Science (New York, N.Y.), (10): [PMID:21659603] |
