| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab176170-10μg | 10μg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $56.90 | |
| Ab176170-50μg | 50μg | In stock | $159.90 | |
| Ab176170-100μg | 100μg | In stock | $249.90 | |
| Ab176170-1mg | 1mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $999.90 |
mAb( 3D4-1); Mouse anti Human RBP4 Antibody; Detection Antibody, ELISA, CLIA, LF, GICA, FIA; Unconjugated
| Product Name | RBP4 Mouse mAb - Primary antibody, specific to RBP4, Mouse IgG1 |
|---|---|
| Synonyms | Plasma retinol-binding protein | PRBP | RBP |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Azide Free, Lot by Lot |
| Host species | Mouse |
| Specificity | RBP4 |
| Immunogen | Recombinant Human RBP4 Protein, C-His tag |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, Validated |
| Product Description | Mouse anti Human RBP4 Antibody, Monoclonal (3D4-1), is a Detection antibody could be used for ELISA, CLIA, LF, GICA, FIA. |
| Isotype | Mouse IgG1 |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Affinity chromatography |
| Matched Pairs | [Ab176167] and [Ab176165] RBP4 Mouse mAb as Capture antibody in ELISA, CLIA, LF, GICA, FIA |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 2-8°C short term (30 days). Store at -20°C long term (36 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Sandwich ELISA - RBP4 protein standard curve
Human RBP4 protein was serially diluted 2-fold and captured by RBP4 Mouse mAb (Ab176165) coated on a Clear Polystyrene Microplate (W291037). RBP4 Mouse mAb (Ab176170) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (np156148).
Relevance of detection results between aladdin Antibody Pair (Ab176165 & Ab176170) and R&D in CLIA
·Capture antibody: RBP4 Mouse mAb (Ab176165)
·Detection antibody: RBP4 Mouse mAb (Ab176170)
Relevance of detection results between aladdin Antibody Pair (Ab176167 & Ab176170) and R&D in CLIA
·Capture antibody: RBP4 Mouse mAb (Ab176167)
·Detection antibody: RBP4 Mouse mAb (Ab176170)
Binding ELISA - RBP4 Mouse mAb (Ab176170)
Immobilized Recombinant Human RBP4 Protein at 1.0 μg/mL can bind RBP4 Mouse mAb (Ab176170) with the EC₅₀ of 22.69 ng/mL.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
| Application | Dilution info |
|---|---|
| CLIA | 100μg/4μg AE |
| FLFIA | 1.0 mg/mL |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F1101571 | Certificate of Analysis | Nov 08, 2023 | Ab176170 |
| ZJ23F1101572 | Certificate of Analysis | Nov 08, 2023 | Ab176170 |
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| 2. Cioffi CL, Dobri N, Freeman EE, Conlon MP, Chen P, Stafford DG, Schwarz DM, Golden KC, Zhu L, Kitchen DB, Barnes KD, Racz B, Qin Q, Michelotti E, Cywin CL, Martin WH, Pearson PG, Johnson G, Petrukhin K.. (2014) Design, synthesis, and evaluation of nonretinoid retinol binding protein 4 antagonists for the potential treatment of atrophic age-related macular degeneration and Stargardt disease.. J Med Chem, 57 (18): (7731-7757). [PMID:25210858] [10.1021/jm5010013] |
| 3. Cioffi CL, Racz B, Freeman EE, Conlon MP, Chen P, Stafford DG, Schwarz DM, Zhu L, Kitchen DB, Barnes KD, Dobri N, Michelotti E, Cywin CL, Martin WH, Pearson PG, Johnson G, Petrukhin K.. (2015) Bicyclic [3.3.0]-Octahydrocyclopenta[c]pyrrolo Antagonists of Retinol Binding Protein 4: Potential Treatment of Atrophic Age-Related Macular Degeneration and Stargardt Disease.. J Med Chem, 58 (15): (5863-5888). [PMID:26181715] [10.1021/acs.jmedchem.5b00423] |
| 4. Cioffi CL, Racz B, Varadi A, Freeman EE, Conlon MP, Chen P, Zhu L, Kitchen DB, Barnes KD, Martin WH, Pearson PG, Johnson G, Blaner WS, Petrukhin K.. (2019) Design, Synthesis, and Preclinical Efficacy of Novel Nonretinoid Antagonists of Retinol-Binding Protein 4 in the Mouse Model of Hepatic Steatosis.. J Med Chem, 62 (11): (5470-5500). [PMID:31079449] [10.1021/acs.jmedchem.9b00352] |
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| 24. Berry, Daniel C DC, O'Byrne, Sheila M SM, Vreeland, Amanda C AC, Blaner, William S WS and Noy, Noa N.. (2012) Cross talk between signaling and vitamin A transport by the retinol-binding protein receptor STRA6.. Molecular and cellular biology, [PMID:22665496] |
| 25. Cukras, Catherine C and 11 more authors.. (2012) Exome analysis identified a novel mutation in the RBP4 gene in a consanguineous pedigree with retinal dystrophy and developmental abnormalities.. PloS one, [PMID:23189188] |
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