| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab086603-10μl | 10μl | In stock | $66.90 | |
| Ab086603-50μl | 50μl | In stock | $199.90 | |
| Ab086603-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| Ab086603-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,699.90 |
Recombinant; Rabbit anti Human 14-3-3 gamma/YWHAG Antibody; WB; Unconjugated
| Product Name | Recombinant 14-3-3 gamma/YWHAG Antibody - Primary antibody, specific to YWHAG, Rabbit IgG |
|---|---|
| Synonyms | 1433 gamma | 14-3-3 gamma | 14-3-3 protein gamma | 14-3-3GAMMA | KCIP-1 | Protein kinase C inhibitor protein 1 | tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gammapolypeptide | YWHAG | 14 3 3 gamma antibody | 14 3 3 protein gamm |
| Specifications & Purity | Recombinant, ExactAb™, Validated, KD, 0.25 mg/mL |
| Host species | Rabbit |
| Specificity | YWHAG |
| Immunogen | A synthetic peptide derived from human 14-3-3 gamma (AA 135-186). |
| Positive Control | WB: HeLa, A549, A431 K562, Jurkat, NIH/3T3 and C6 cell lysates. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, KD Validation, Recombinant, Validated |
| Product Description |
Rabbit anti Human 14-3-3 gamma/YWHAG Antibody, Recombinant, could be used for WB and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 0.25 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant 14-3-3 gamma/YWHAG Antibody (Ab086603) - Western Blot
All lanes: Recombinant 14-3-3 gamma/YWHAG Antibody (Ab086603) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa
Exposure time: 41.0 s
Recombinant 14-3-3 gamma/YWHAG Antibody (Ab086603) - Western Blot
All lanes: Recombinant 14-3-3 gamma/YWHAG Antibody (Ab086603) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa
Exposure time: 17.0 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000"> 1/500-1/1000 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0202977 | Certificate of Analysis | Feb 29, 2024 | Ab086603 |
| ZJ24F0202976 | Certificate of Analysis | Feb 29, 2024 | Ab086603 |
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| 7. Ganguly, S S and 13 more authors.. (2001) Role of a pineal cAMP-operated arylalkylamine N-acetyltransferase/14-3-3-binding switch in melatonin synthesis.. Proceedings of the National Academy of Sciences of the United States of America, (3): [PMID:11427721] |
| 8. Gevaert, Kris K and 6 more authors.. (2003) Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides.. Nature biotechnology, [PMID:12665801] |
| 9. Hillier, Ladeana W LW and 106 more authors.. (2003) The DNA sequence of human chromosome 7.. Nature, (10): [PMID:12853948] |
| 10. Andersen, Jens S JS and 5 more authors.. (2003) Proteomic characterization of the human centrosome by protein correlation profiling.. Nature, (4): [PMID:14654843] |
| 11. Nagata-Ohashi, Kyoko K and 11 more authors.. (2004) A pathway of neuregulin-induced activation of cofilin-phosphatase Slingshot and cofilin in lamellipodia.. The Journal of cell biology, (24): [PMID:15159416] |
| 12. Screaton, Robert A RA and 11 more authors.. (2004) The CREB coactivator TORC2 functions as a calcium- and cAMP-sensitive coincidence detector.. Cell, (1): [PMID:15454081] |
| 13. Yoshida, Kiyotsugu K, Yamaguchi, Tomoko T, Natsume, Tohru T, Kufe, Donald D and Miki, Yoshio Y.. (2005) JNK phosphorylation of 14-3-3 proteins regulates nuclear targeting of c-Abl in the apoptotic response to DNA damage.. Nature cell biology, [PMID:15696159] |
| 14. Jin, Yetao Y and 6 more authors.. (2006) 14-3-3gamma binds to MDMX that is phosphorylated by UV-activated Chk1, resulting in p53 activation.. The EMBO journal, (22): [PMID:16511572] |
| 15. Angrand, Pierre-Olivier PO and 12 more authors.. (2006) Transgenic mouse proteomics identifies new 14-3-3-associated proteins involved in cytoskeletal rearrangements and cell signaling.. Molecular & cellular proteomics : MCP, [PMID:16959763] |
| 16. Yang, Xiaowen X and 8 more authors.. (2006) Structural basis for protein-protein interactions in the 14-3-3 protein family.. Proceedings of the National Academy of Sciences of the United States of America, (14): [PMID:17085597] |
| 17. Jin, Yun-Hye YH and 6 more authors.. (2008) Sirt2 interacts with 14-3-3 beta/gamma and down-regulates the activity of p53.. Biochemical and biophysical research communications, (11): [PMID:18249187] |
| 18. Brummer, Tilman T and 11 more authors.. (2008) Phosphorylation-dependent binding of 14-3-3 terminates signalling by the Gab2 docking protein.. The EMBO journal, (3): [PMID:19172738] |
| 19. Kajiwara, Yuji Y, Buxbaum, Joseph D JD and Grice, Dorothy E DE.. (2009) SLITRK1 binds 14-3-3 and regulates neurite outgrowth in a phosphorylation-dependent manner.. Biological psychiatry, (15): [PMID:19640509] |
| 20. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332] |
| 21. Olsen, Jesper V JV and 11 more authors.. (2010) Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis.. Science signaling, (12): [PMID:20068231] |
| 22. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
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| 25. Yuasa, Keizo K and 5 more authors.. (2015) Suppression of death-associated protein kinase 2 by interaction with 14-3-3 proteins.. Biochemical and biophysical research communications, (14): [PMID:26047703] |
| 26. Masters, Seth L SL and 31 more authors.. (2016) Familial autoinflammation with neutrophilic dermatosis reveals a regulatory mechanism of pyrin activation.. Science translational medicine, (30): [PMID:27030597] |
| 27. Stevers, Loes M LM and 5 more authors.. (2017) Structural interface between LRRK2 and 14-3-3 protein.. The Biochemical journal, (23): [PMID:28202711] |
| 28. Guella, Ilaria I and 17 more authors.. (2017) De Novo Mutations in YWHAG Cause Early-Onset Epilepsy.. American journal of human genetics, (3): [PMID:28777935] |
