| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab086791-10μl | 10μl | In stock | $66.90 | |
| Ab086791-50μl | 50μl | In stock | $199.90 | |
| Ab086791-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $339.90 | |
| Ab086791-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,889.90 |
Recombinant; Rabbit anti Human 5T4 Antibody; WB, IHC; Unconjugated
| Product Name | Recombinant 5T4 Antibody - Primary antibody, specific to TPBG, Rabbit IgG |
|---|---|
| Synonyms | 5T4 oncofetal trophoblast glycoprotein | 5T4 oncotrophoblast glycoprotein | 5T4 | 5T4AG | 5T4-AG | 5T4M6P1,5T4 oncofetal antigen | M6P1 | TPBG | trophoblast glycoprotein | 5T4 antibody | 5T4 oncofetal antigen antibody | 5T4 oncofetal trophoblast glycoprot |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.23 mg/mL |
| Host species | Rabbit |
| Specificity | TPBG |
| Immunogen | A synthetic peptide derived from human 5T4 (AA 110-170). |
| Positive Control | WB: A549, HT-29 and MCF7 cell lysates. IHC: Human uters cancer tissue. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human 5T4 Antibody, Recombinant, could be used for WB, IHC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 0.23 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant 5T4 Antibody (Ab086791) - Western Blot
All lanes: Recombinant 5T4 Antibody (Ab086791) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 46 kDa
Observed band size: 78 kDa
Exposure time: 162.1 s
Recombinant 5T4 Antibody (Ab086791) - Western Blot
All lanes: Recombinant 5T4 Antibody (Ab086791) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 46 kDa
Observed band size: 78 kDa
Exposure time: 10.0 s
Recombinant 5T4 Antibody (Ab086791) - IHC
Immunohistochemistry analysis of paraffin-embedded Human uters cancer using Recombinant 5T4 Antibody (Ab086791). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000"> 1/500-1/1000 |
| IHC | 1/50 - 1/100 "> 1/50 - 1/100 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0303230 | Certificate of Analysis | Mar 14, 2024 | Ab086791 |
| ZJ24F0303231 | Certificate of Analysis | Mar 14, 2024 | Ab086791 |
| 1. Myers KA, Rahi-Saund V, Davison MD, Young JA, Cheater AJ, Stern PL. (1994) Isolation of a cDNA encoding 5T4 oncofetal trophoblast glycoprotein. An antigen associated with metastasis contains leucine-rich repeats.. J Biol Chem, 269 (12): (9319-24). [PMID:8132670] [10.1021/op500134e] |
| 2. Forsberg G, Ohlsson L, Brodin T, Björk P, Lando PA, Shaw D, Stern PL, Dohlsten M. (2001) Therapy of human non-small-cell lung carcinoma using antibody targeting of a modified superantigen.. Br J Cancer, 85 (1): (129-36). [PMID:11437414] [10.1021/op500134e] |
| 3. Mulryan, Kate K and 7 more authors.. (2002) Attenuated recombinant vaccinia virus expressing oncofetal antigen (tumor-associated antigen) 5T4 induces active therapy of established tumors.. Molecular cancer therapeutics, [PMID:12481437] |
| 4. Otsuki, Tetsuji T and 24 more authors.. (2005) Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries.. DNA research : an international journal for rapid publication of reports on genes and genomes, [PMID:16303743] |
| 5. Zhao, Yuguang Y, Malinauskas, Tomas T, Harlos, Karl K and Jones, E Yvonne EY.. (2014) Structural insights into the inhibition of Wnt signaling by cancer antigen 5T4/Wnt-activated inhibitory factor 1.. Structure (London, England : 1993), (8): [PMID:24582434] |
