| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab088446-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $69.90 | |
| Ab088446-50μl | 50μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $207.90 | |
| Ab088446-100μl | 100μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $297.90 | |
| Ab088446-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,187.90 |
Recombinant (RR645); Rabbit anti Human ACTA2 Antibody; WB, IHC, Flow, IF, ICC; Unconjugated
| Product Name | Recombinant ACTA2 Antibody - Primary antibody, specific to ACTA2, Rabbit IgG |
|---|---|
| Synonyms | a actin antibody | AAT6 antibody | ACTA_HUMAN antibody | ACTA2 antibody | Actin alpha 2 smooth muscle aorta antibody | Actin aortic smooth muscle antibody | Actin, aortic smooth muscle antibody | ACTSA antibody | ACTVS antibody | Alpha 2 actin antibody | |
| Specifications & Purity | ExactAb™, Validated, Recombinant, High performance, 0.05 mg/mL |
| Host species | Rabbit |
| Specificity | ACTA2 |
| Immunogen | A synthetic peptide corresponding to residues on the N-terminus of human Alpha Actin 2 was used as an immunogen. |
| Positive Control | WB: Hela, HEK293, LNCap, Raji, Hep G2, A431, Jurkat, HUVEC, C6, NIH/3T3 cell lyeates. IHC: Placenta. Flow: HeLa cells. IF/ICC: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, High Performance, Recombinant, Validated |
| Product Description | Rabbit anti Human ACTA2 Antibody, Recombinant (RR645), could be used for WB, IHC, Flow, IF, ICC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 0.05 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant ACTA2 Antibody (Ab088446) - Western Blot
All lanes: Recombinant ACTA2 Antibody (Ab088446) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 44 kDa
Exposure time: 80.0 s
Recombinant ACTA2 Antibody (Ab088446) - IF
IF analysis of ACTA2 (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained Recombinant ACTA2 Antibody (Ab088446) at 1/100 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.
Recombinant ACTA2 Antibody (Ab088446) - IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of placenta tissue labelling ACTA2 with Recombinant ACTA2 Antibody (Ab088446) at 1/2000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.
Recombinant ACTA2 Antibody (Ab088446) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Recombinant ACTA2 Antibody (Ab088446) (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in Recombinant ACTA2 Antibody (Ab088446) (1/100 dilution) in 1x PBS/1% BSA for 30 min at 4°C. The secondary antibody used was a Goat Anti-Rabbit Alexa Fluor® 488 (IgG H+L) at 1/2000 dilution for 20 min at 4°C. Unlabeled sample (Green) was used as a control.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/1000 - 1/2000"> 1/1000 - 1/2000 |
| IF/ICC | 1/80 - 1/250 |
| Flow Cytometry | 1/20 - 1/100 |
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F0901100 | Certificate of Analysis | Sep 18, 2023 | Ab088446 |
| ZJ23F0901101 | Certificate of Analysis | Sep 18, 2023 | Ab088446 |
