| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab087460-10μl | 10μl | In stock | $76.90 | |
| Ab087460-50μl | 50μl | In stock | $229.90 | |
| Ab087460-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $369.90 | |
| Ab087460-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,999.90 |
Recombinant; Rabbit anti Human ADAR1 Antibody; WB; Unconjugated
| Product Name | Recombinant ADAR1 Antibody - Primary antibody, specific to ADAR, Rabbit IgG |
|---|---|
| Synonyms | 136 kDa double-stranded RNA-binding protein | 136kDa double stranded RNA binding protein | Adar 1 | ADAR | Adar1 | Adenosine deaminase acting on RNA 1 A | Adenosine deaminase RNA specific 1 | Adenosine deaminase RNA specific | Adenosine deaminase that act |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.18 mg/mL |
| Host species | Rabbit |
| Specificity | ADAR |
| Immunogen | A synthetic peptide derived from human ADAR1 (AA 200-250) |
| Positive Control | WB: HeLa, Hep G2 and SK-N-SH cell lysates. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human ADAR1 Antibody, Recombinant, could be used for WB and so on. |
| Isotype | Rabbit IgG |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 0.18 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant ADAR1 Antibody (Ab087460) - Western Blot
All lanes: Recombinant ADAR1 Antibody (Ab087460) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 136 kDa
Observed band size: 138, 150 kDa
Exposure time: 83.2 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0505442 | Certificate of Analysis | May 13, 2024 | Ab087460 |
| ZJ24F0505441 | Certificate of Analysis | May 13, 2024 | Ab087460 |
| 1. Francis Impens,Lilliana Radoshevich,Pascale Cossart,David Ribet. (2014-08-13) Mapping of SUMO sites and analysis of SUMOylation changes induced by external stimuli.. Proceedings of the National Academy of Sciences of the United States of America, 111 ((34)): (12432-12437). [PMID:25114211] |
| 2. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330] |
| 3. Patterson, J B JB and Samuel, C E CE.. (1995) Expression and regulation by interferon of a double-stranded-RNA-specific adenosine deaminase from human cells: evidence for two forms of the deaminase.. Molecular and cellular biology, [PMID:7565688] |
| 4. Kim, U U, Wang, Y Y, Sanford, T T, Zeng, Y Y and Nishikura, K K.. (1994) Molecular cloning of cDNA for double-stranded RNA adenosine deaminase, a candidate enzyme for nuclear RNA editing.. Proceedings of the National Academy of Sciences of the United States of America, (22): [PMID:7972084] |
| 5. Liu, Y Y, George, C X CX, Patterson, J B JB and Samuel, C E CE.. (1997) Functionally distinct double-stranded RNA-binding domains associated with alternative splice site variants of the interferon-inducible double-stranded RNA-specific adenosine deaminase.. The Journal of biological chemistry, (14): [PMID:9020165] |
| 6. George, C X CX and Samuel, C E CE.. (1999) Human RNA-specific adenosine deaminase ADAR1 transcripts possess alternative exon 1 structures that initiate from different promoters, one constitutively active and the other interferon inducible.. Proceedings of the National Academy of Sciences of the United States of America, (13): [PMID:10200312] |
| 7. Schwartz, T T, Rould, M A MA, Lowenhaupt, K K, Herbert, A A and Rich, A A.. (1999) Crystal structure of the Zalpha domain of the human editing enzyme ADAR1 bound to left-handed Z-DNA.. Science (New York, N.Y.), (11): [PMID:10364558] |
| 8. Schade, M M and 7 more authors.. (1999) The solution structure of the Zalpha domain of the human RNA editing enzyme ADAR1 reveals a prepositioned binding surface for Z-DNA.. Proceedings of the National Academy of Sciences of the United States of America, (26): [PMID:10535945] |
| 9. Cho, Dan-Sung C DS and 5 more authors.. (2003) Requirement of dimerization for RNA editing activity of adenosine deaminases acting on RNA.. The Journal of biological chemistry, (9): [PMID:12618436] |
| 10. Desterro, Joana M P JM and 5 more authors.. (2003) Dynamic association of RNA-editing enzymes with the nucleolus.. Journal of cell science, (1): [PMID:12665561] |
| 11. Miyamura, Yoshinori Y and 6 more authors.. (2003) Mutations of the RNA-specific adenosine deaminase gene (DSRAD) are involved in dyschromatosis symmetrica hereditaria.. American journal of human genetics, [PMID:12916015] |
| 12. Zhang, Xue-Jun XJ and 14 more authors.. (2004) Seven novel mutations of the ADAR gene in Chinese families and sporadic patients with dyschromatosis symmetrica hereditaria (DSH).. Human mutation, [PMID:15146470] |
| 13. Yang, Weidong W and 6 more authors.. (2005) ADAR1 RNA deaminase limits short interfering RNA efficacy in mammalian cells.. The Journal of biological chemistry, (4): [PMID:15556947] |
| 14. Li, Cheng-Rang CR and 9 more authors.. (2005) A new arginine substitution mutation of DSRAD gene in a Chinese family with dyschromatosis symmetrica hereditaria.. Journal of dermatological science, [PMID:15659327] |
| 15. Taylor, Deborah R DR, Puig, Montserrat M, Darnell, Miriam E R ME, Mihalik, Kathleen K and Feinstone, Stephen M SM.. (2005) New antiviral pathway that mediates hepatitis C virus replicon interferon sensitivity through ADAR1.. Journal of virology, [PMID:15858013] |
| 16. Nie, Yongzhan Y, Ding, Li L, Kao, Peter N PN, Braun, Robert R and Yang, Jing-Hua JH.. (2005) ADAR1 interacts with NF90 through double-stranded RNA and regulates NF90-mediated gene expression independently of RNA editing.. Molecular and cellular biology, [PMID:16055709] |
| 17. Desterro, Joana M P JM and 5 more authors.. (2005) SUMO-1 modification alters ADAR1 editing activity.. Molecular biology of the cell, [PMID:16120648] |
| 18. Hartwig, D D and 6 more authors.. (2006) The large form of ADAR 1 is responsible for enhanced hepatitis delta virus RNA editing in interferon-alpha-stimulated host cells.. Journal of viral hepatitis, [PMID:16475990] |
| 19. Gregory, S G SG and 178 more authors.. (2006) The DNA sequence and biological annotation of human chromosome 1.. Nature, (18): [PMID:16710414] |
| 20. Sjöblom, Tobias T and 28 more authors.. (2006) The consensus coding sequences of human breast and colorectal cancers.. Science (New York, N.Y.), (13): [PMID:16959974] |
| 21. Beausoleil, Sean A SA, Villén, Judit J, Gerber, Scott A SA, Rush, John J and Gygi, Steven P SP.. (2006) A probability-based approach for high-throughput protein phosphorylation analysis and site localization.. Nature biotechnology, [PMID:16964243] |
| 22. Nie, Yongzhan Y, Hammond, Graeme L GL and Yang, Jing-Hua JH.. (2007) Double-stranded RNA deaminase ADAR1 increases host susceptibility to virus infection.. Journal of virology, [PMID:17079286] |
| 23. Yu, Li-Rong LR and 5 more authors.. (2007) Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra.. Journal of proteome research, [PMID:17924679] |
| 24. Bechtel, Stephanie S and 18 more authors.. (2007) The full-ORF clone resource of the German cDNA Consortium.. BMC genomics, (31): [PMID:17974005] |
| 25. Cenci, Caterina C and 8 more authors.. (2008) Down-regulation of RNA editing in pediatric astrocytomas: ADAR2 editing activity inhibits cell migration and proliferation.. The Journal of biological chemistry, (14): [PMID:18178553] |
| 26. Cantin, Greg T GT and 6 more authors.. (2008) Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis.. Journal of proteome research, [PMID:18220336] |
| 27. Agranat, Lily L, Raitskin, Oleg O, Sperling, Joseph J and Sperling, Ruth R.. (2008) The editing enzyme ADAR1 and the mRNA surveillance protein hUpf1 interact in the cell nucleus.. Proceedings of the National Academy of Sciences of the United States of America, (1): [PMID:18362360] |
| 28. Dephoure, Noah N and 6 more authors.. (2008) A quantitative atlas of mitotic phosphorylation.. Proceedings of the National Academy of Sciences of the United States of America, (5): [PMID:18669648] |
| 29. Fritz, Jutta J and 5 more authors.. (2009) RNA-regulated interaction of transportin-1 and exportin-5 with the double-stranded RNA-binding domain regulates nucleocytoplasmic shuttling of ADAR1.. Molecular and cellular biology, [PMID:19124606] |
| 30. Clerzius, Guerline G and 5 more authors.. (2009) ADAR1 interacts with PKR during human immunodeficiency virus infection of lymphocytes and contributes to viral replication.. Journal of virology, [PMID:19605474] |
| 31. Doria, Margherita M, Neri, Francesca F, Gallo, Angela A, Farace, Maria Giulia MG and Michienzi, Alessandro A.. (2009) Editing of HIV-1 RNA by the double-stranded RNA deaminase ADAR1 stimulates viral infection.. Nucleic acids research, [PMID:19651874] |
| 32. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332] |
| 33. Toth, Ann M AM, Li, Zhiqun Z, Cattaneo, Roberto R and Samuel, Charles E CE.. (2009) RNA-specific adenosine deaminase ADAR1 suppresses measles virus-induced apoptosis and activation of protein kinase PKR.. The Journal of biological chemistry, (23): [PMID:19710021] |
| 34. Galeano, Federica F and 9 more authors.. (2010) Human BLCAP transcript: new editing events in normal and cancerous tissues.. International journal of cancer, (1): [PMID:19908260] |
| 35. and Nishikura, Kazuko.. (2010) Functions and regulation of RNA editing by ADAR deaminases.. Annual review of biochemistry, [PMID:20192758] |
| 36. and Samuel, Charles E.. (2011) Adenosine deaminases acting on RNA (ADARs) are both antiviral and proviral.. Virology, (15): [PMID:21211811] |
| 37. Doria, Margherita M and 6 more authors.. (2011) ADAR2 editing enzyme is a novel human immunodeficiency virus-1 proviral factor.. The Journal of general virology, [PMID:21289159] |
| 38. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692] |
| 39. Gélinas, Jean-François JF, Clerzius, Guerline G, Shaw, Eileen E and Gatignol, Anne A.. (2011) Enhancement of replication of RNA viruses by ADAR1 via RNA editing and inhibition of RNA-activated protein kinase.. Journal of virology, [PMID:21490091] |
| 40. and Wang, Qingde Q.. (2011) RNA editing catalyzed by ADAR1 and its function in mammalian cells.. Biochemistry. Biokhimiia, [PMID:22022963] |
| 41. Li, Zhiqun Z, Okonski, Kristina M KM and Samuel, Charles E CE.. (2012) Adenosine deaminase acting on RNA 1 (ADAR1) suppresses the induction of interferon by measles virus.. Journal of virology, [PMID:22278222] |
| 42. Mallela, Arka A and Nishikura, Kazuko K.. () A-to-I editing of protein coding and noncoding RNAs.. Critical reviews in biochemistry and molecular biology, [PMID:22988838] |
| 43. Rice, Gillian I GI and 53 more authors.. (2012) Mutations in ADAR1 cause Aicardi-Goutières syndrome associated with a type I interferon signature.. Nature genetics, [PMID:23001123] |
| 44. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
| 45. Guo, Ailan and 15 more authors.. (2014) Immunoaffinity enrichment and mass spectrometry analysis of protein methylation.. Molecular & cellular proteomics : MCP, [PMID:24129315] |
| 46. Bian, Yangyang Y and 9 more authors.. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.. Journal of proteomics, (16): [PMID:24275569] |
| 47. Barraud, Pierre P, Banerjee, Silpi S, Mohamed, Weaam I WI, Jantsch, Michael F MF and Allain, Frédéric H-T FH.. (2014) A bimodular nuclear localization signal assembled via an extended double-stranded RNA-binding domain acts as an RNA-sensing signal for transportin 1.. Proceedings of the National Academy of Sciences of the United States of America, (6): [PMID:24753571] |
| 48. Xiao, Zhenyu Z and 5 more authors.. (2015) System-wide Analysis of SUMOylation Dynamics in Response to Replication Stress Reveals Novel Small Ubiquitin-like Modified Target Proteins and Acceptor Lysines Relevant for Genome Stability.. Molecular & cellular proteomics : MCP, [PMID:25755297] |
| 49. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460] |
