Recombinant AKT1/AKT2/AKT3 Antibody - Primary antibody, specific to AKT1/AKT2/AKT3, IgG

    Application:
  • IHC
  • WB
Features and benefits
  • Host species: Rabbit
  • Species reactivity(Reacts with): Hamster,Human,Mouse,Rat
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
Item Number
Ab087925
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab087925-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$66.90
Ab087925-50μl
50μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$189.90
Ab087925-100μl
100μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$299.90
Ab087925-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,249.90

Recombinant; Rabbit anti Human AKT1/AKT2/AKT3 Antibody; WB, IHC; Unconjugated

Basic Description

Product NameRecombinant AKT1/AKT2/AKT3 Antibody - Primary antibody, specific to AKT1/AKT2/AKT3, IgG
SynonymsAKT antibody | AKT1 antibody | AKT1 kinase antibody | AKT1m antibody | AKT2 antibody | AKT2 kinase antibody | Akt3 antibody | AKT3_HUMAN antibody | CAKT antibody | CWS6 antibody | DKFZp434N0250 antibody | HIHGHH antibody | kinase Akt1 antibody | MGC99656
Specifications & PurityExactAb™, Validated, Recombinant, 1.1 mg/mL
Host speciesRabbit
SpecificityAKT1/AKT2/AKT3
ImmunogenRecombinant protein of human AKT1/2/3 (AA 281-480).
Positive ControlWB: HeLa, Jurkat, NIH/3T3, A549 and MCF7 cell lysates. IHC: Human lung cancer tissue.
ConjugationUnconjugated
GradeExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human AKT1/AKT2/AKT3 Antibody, Recombinant, could be used for WB, IHC and so on.
Application
• WB: 1/500-1/1000
• IHC: 1/50-1/100
Protein Function
IGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent.

Product Properties

IsotypeIgG
SDS-PAGE150 kDa
Purification MethodAntigen affinity purification
FormLiquid
Concentration1.1 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant AKT1/AKT2/AKT3 Antibody (Ab087925) - Western Blot
All lanes: Recombinant AKT1/AKT2/AKT3 Antibody (Ab087925) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 56 kDa
Observed band size: 57 kDa
Exposure time: 2.7 s

Recombinant AKT1/AKT2/AKT3 Antibody (Ab087925) - IHC
Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using Recombinant AKT1/AKT2/AKT3 Antibody (Ab087925). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Associated Targets(Human)

AKT3 Tchem RAC-gamma serine/threonine-protein kinase (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
AKT2 Tchem RAC-beta serine/threonine-protein kinase (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
AKT1 Tchem RAC-alpha serine/threonine-protein kinase (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
AKT2 Tchem Serine/threonine-protein kinase AKT2 (4301 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
AKT3 Tchem Serine/threonine-protein kinase AKT3 (3157 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
AKT1 Tchem Serine/threonine-protein kinase AKT (9192 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
AKT2 Tchem Serine/threonine-protein kinase AKT (245 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
AKT2 Tchem Serine/threonine-protein kinase Rac alpha/beta (1 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
AKT1 Tchem VHL/AKT1 (26 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
AKT1 Tchem CRBN/AKT1 (26 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
AKT2 Tchem CRBN/AKT2 (2 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID

Mechanisms of Action

Mechanism of ActionAction Typetarget IDTarget NameTarget TypeTarget OrganismBinding Site NameReferences

Application

ApplicationDilution info
WB1/500-1/1000

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1/500-1/1000

IHC1/50 - 1/100

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1/50 - 1/100

Certificates

Certificate of Analysis(COA)

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2 results found

Lot NumberCertificate TypeDateItem
ZJ23F0901115Certificate of AnalysisSep 24, 2023 Ab087925
ZJ23F0901114Certificate of AnalysisSep 24, 2023 Ab087925

Related Documents

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