Recombinant protein of human AKT1/2/3 (AA 281-480).
Positive Control
WB: HeLa, Jurkat, NIH/3T3, A549 and MCF7 cell lysates. IHC: Human lung cancer tissue.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human AKT1/AKT2/AKT3 Antibody, Recombinant, could be used for WB, IHC and so on. Application • WB: 1/500-1/1000 • IHC: 1/50-1/100 Protein Function IGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent.
Product Properties
Isotype
IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purification
Form
Liquid
Concentration
1.1 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant AKT1/AKT2/AKT3 Antibody (Ab087925) - Western Blot All lanes: Recombinant AKT1/AKT2/AKT3 Antibody (Ab087925) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 56 kDa Observed band size: 57 kDa Exposure time: 2.7 s
Recombinant AKT1/AKT2/AKT3 Antibody (Ab087925) - IHC Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using Recombinant AKT1/AKT2/AKT3 Antibody (Ab087925). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.