Basic Description

Product Name	Recombinant ALIX Antibody - Primary antibody, specific to PDCD6IP, Rabbit IgG
Synonyms	PDC6I_HUMAN \| Programmed cell death 6-interacting protein \| AIP1 \| ALIX \| KIAA1375 \| PDCD6-interacting protein \| ALG-2-interacting protein 1 \| ALG-2-interacting protein X \| Hp95 \| AIP1 antibody \| ALG 2 interacting protein 1 antibody \| ALG-2-interacting pr
Specifications & Purity	ExactAb™, Validated, Recombinant, 1.2 mg/mL
Host species	Rabbit
Specificity	PDCD6IP
Immunogen	A synthetic peptide derived from human ALIX (AA 3-102)
Positive Control	WB: HeLa, MCF7, Jurkat, A549, K562, NIH/3T3 and C6 cell lysates.
Conjugation	Unconjugated
Grade	ExactAb™, Recombinant, Validated
Product Description	Rabbit anti Human ALIX Antibody, Recombinant, could be used for WB and so on. Application: WB: 1/500-1/1000 Protein Function Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome. Binds to the phospholipid lysobisphosphatidic acid (LBPA) which is abundant in MVBs internal membranes. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). Appears to be an adapter for a subset of ESCRT-III proteins, such as CHMP4, to function at distinct membranes. Required for completion of cytokinesis. Involved in HIV-1 virus budding. Can replace TSG101 it its role of supporting HIV-1 release; this function implies the interaction with CHMP4B. May play a role in the regulation of both apoptosis and cell proliferation.

Product Properties

Isotype	Rabbit IgG
SDS-PAGE	150 kDa
Purification Method	Antigen affinity purified
Form	Liquid
Concentration	1.2 mg/mL
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant ALIX Antibody (Ab088156) - Western Blot
All lanes: Recombinant ALIX Antibody (Ab088156) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution

Predicted band size: 96 kDa
Observed band size: 81 kDa
Exposure time: 7.0 s

Recombinant ALIX Antibody (Ab088156) - Western Blot
All lanes: Recombinant ALIX Antibody (Ab088156) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution

Predicted band size: 96 kDa
Observed band size: 80, 100 kDa
Exposure time: 25.0 s

Associated Targets(Human)

PDCD6IP Tbio Programmed cell death 6-interacting protein (0 Activities)

Discover Target: PDCD6IP

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Application

Application	Dilution info
WB	1/500-1/1000

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0303220	Certificate of Analysis	Mar 14, 2024	Ab088156
ZJ24F0303221	Certificate of Analysis	Mar 14, 2024	Ab088156

FAQs and Articles

References

1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M. (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.. Science, 325 (5942): (834-40). [PMID:19608861]
2. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330]
3. Nagase, T T, Kikuno, R R, Ishikawa, K I KI, Hirosawa, M M and Ohara, O O.. (2000) Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro.. DNA research : an international journal for rapid publication of reports on genes and genomes, (28): [PMID:10718198]
4. Wu, Y Y and 6 more authors.. (2001) Overexpression of Hp95 induces G1 phase arrest in confluent HeLa cells.. Differentiation; research in biological diversity, [PMID:11683497]
5. Katoh, Keiichi K and 7 more authors.. (2003) The ALG-2-interacting protein Alix associates with CHMP4b, a human homologue of yeast Snf7 that is involved in multivesicular body sorting.. The Journal of biological chemistry, (3): [PMID:12860994]
6. Strack, Bettina B, Calistri, Arianna A, Craig, Stewart S, Popova, Elena E and Göttlinger, Heinrich G HG.. (2003) AIP1/ALIX is a binding partner for HIV-1 p6 and EIAV p9 functioning in virus budding.. Cell, (19): [PMID:14505569]
7. von Schwedler, Uta K UK and 14 more authors.. (2003) The protein network of HIV budding.. Cell, (19): [PMID:14505570]
8. Martin-Serrano, Juan J, Yarovoy, Anton A, Perez-Caballero, David D, Bieniasz, Paul D PD and Yaravoy, Anton A.. (2003) Divergent retroviral late-budding domains recruit vacuolar protein sorting factors by using alternative adaptor proteins.. Proceedings of the National Academy of Sciences of the United States of America, (14): [PMID:14519844]
9. Peck, Jeremy W JW, Bowden, Emma T ET and Burbelo, Peter D PD.. (2004) Structure and function of human Vps20 and Snf7 proteins.. The Biochemical journal, (1): [PMID:14583093]
10. Katoh, Keiichi K, Shibata, Hideki H, Hatta, Kazumi K and Maki, Masatoshi M.. (2004) CHMP4b is a major binding partner of the ALG-2-interacting protein Alix among the three CHMP4 isoforms.. Archives of biochemistry and biophysics, (1): [PMID:14678797]
11. Matsuo, Hirotami H and 12 more authors.. (2004) Role of LBPA and Alix in multivesicular liposome formation and endosome organization.. Science (New York, N.Y.), (23): [PMID:14739459]
12. Ichioka, Fumitaka F and 5 more authors.. (2005) Identification of Rab GTPase-activating protein-like protein (RabGAPLP) as a novel Alix/AIP1-interacting protein.. Bioscience, biotechnology, and biochemistry, [PMID:15849434]
13. Segura-Morales, Carolina C and 5 more authors.. (2005) Tsg101 and Alix interact with murine leukemia virus Gag and cooperate with Nedd4 ubiquitin ligases during budding.. The Journal of biological chemistry, (22): [PMID:15908698]
14. Muzny, Donna M DM and 113 more authors.. (2006) The DNA sequence, annotation and analysis of human chromosome 3.. Nature, (27): [PMID:16641997]
15. Yamasaki, Akinori A, Tani, Katsuko K, Yamamoto, Akitsugu A, Kitamura, Naomi N and Komada, Masayuki M.. (2006) The Ca2+-binding protein ALG-2 is recruited to endoplasmic reticulum exit sites by Sec31A and stabilizes the localization of Sec31A.. Molecular biology of the cell, [PMID:16957052]
16. Chi, An A and 16 more authors.. (2006) Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes.. Journal of proteome research, [PMID:17081065]
17. Lee, Sangho S, Joshi, Anjali A, Nagashima, Kunio K, Freed, Eric O EO and Hurley, James H JH.. (2007) Structural basis for viral late-domain binding to Alix.. Nature structural & molecular biology, [PMID:17277784]
18. Fisher, Robert D RD and 5 more authors.. (2007) Structural and biochemical studies of ALIX/AIP1 and its role in retrovirus budding.. Cell, (9): [PMID:17350572]
19. Usami, Yoshiko Y, Popov, Sergei S and Göttlinger, Heinrich G HG.. (2007) Potent rescue of human immunodeficiency virus type 1 late domain mutants by ALIX/AIP1 depends on its CHMP4 binding site.. Journal of virology, [PMID:17428861]
20. Carlton, Jez G JG and Martin-Serrano, Juan J.. (2007) Parallels between cytokinesis and retroviral budding: a role for the ESCRT machinery.. Science (New York, N.Y.), (29): [PMID:17556548]
21. Morita, Eiji E and 6 more authors.. (2007) Human ESCRT and ALIX proteins interact with proteins of the midbody and function in cytokinesis.. The EMBO journal, (3): [PMID:17853893]
22. Shibata, Hideki H and 6 more authors.. (2008) Identification of Alix-type and Non-Alix-type ALG-2-binding sites in human phospholipid scramblase 3: differential binding to an alternatively spliced isoform and amino acid-substituted mutants.. The Journal of biological chemistry, (11): [PMID:18256029]
23. McCullough, John J, Fisher, Robert D RD, Whitby, Frank G FG, Sundquist, Wesley I WI and Hill, Christopher P CP.. (2008) ALIX-CHMP4 interactions in the human ESCRT pathway.. Proceedings of the National Academy of Sciences of the United States of America, (3): [PMID:18511562]
24. Carlton, Jez G JG, Agromayor, Monica M and Martin-Serrano, Juan J.. (2008) Differential requirements for Alix and ESCRT-III in cytokinesis and HIV-1 release.. Proceedings of the National Academy of Sciences of the United States of America, (29): [PMID:18641129]
25. Dephoure, Noah N and 6 more authors.. (2008) A quantitative atlas of mitotic phosphorylation.. Proceedings of the National Academy of Sciences of the United States of America, (5): [PMID:18669648]
26. Suzuki, Hironori H and 7 more authors.. (2008) Structural basis for Ca2+ -dependent formation of ALG-2/Alix peptide complex: Ca2+/EF3-driven arginine switch mechanism.. Structure (London, England : 1993), (8): [PMID:18940611]
27. Lee, Hyung Ho HH, Elia, Natalie N, Ghirlando, Rodolfo R, Lippincott-Schwartz, Jennifer J and Hurley, James H JH.. (2008) Midbody targeting of the ESCRT machinery by a noncanonical coiled coil in CEP55.. Science (New York, N.Y.), (24): [PMID:18948538]
28. Okumura, Mayumi M and 6 more authors.. (2009) Penta-EF-hand protein ALG-2 functions as a Ca2+-dependent adaptor that bridges Alix and TSG101.. Biochemical and biophysical research communications, (14): [PMID:19520058]
29. Wang, Pingzhang P, Yu, Peng P, Gao, Peng P, Shi, Taiping T and Ma, Dalong D.. (2009) Discovery of novel human transcript variants by analysis of intronic single-block EST with polyadenylation site.. BMC genomics, (12): [PMID:19906316]
30. Rauch, Susanne S and Martin-Serrano, Juan J.. (2011) Multiple interactions between the ESCRT machinery and arrestin-related proteins: implications for PPXY-dependent budding.. Journal of virology, [PMID:21191027]
31. Benitez, Bruno A BA and 9 more authors.. (2011) Exome-sequencing confirms DNAJC5 mutations as cause of adult neuronal ceroid-lipofuscinosis.. PloS one, [PMID:22073189]
32. Bienvenut, Willy V WV and 6 more authors.. (2012) Comparative large scale characterization of plant versus mammal proteins reveals similar and idiosyncratic N-α-acetylation features.. Molecular & cellular proteomics : MCP, [PMID:22223895]
33. Baietti, Maria Francesca MF and 11 more authors.. (2012) Syndecan-syntenin-ALIX regulates the biogenesis of exosomes.. Nature cell biology, (3): [PMID:22660413]
34. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163]
35. Guo, Ailan and 15 more authors.. (2014) Immunoaffinity enrichment and mass spectrometry analysis of protein methylation.. Molecular & cellular proteomics : MCP, [PMID:24129315]
36. Bian, Yangyang Y and 9 more authors.. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.. Journal of proteomics, (16): [PMID:24275569]
37. Han, Ziying Z and 6 more authors.. (2015) ALIX Rescues Budding of a Double PTAP/PPEY L-Domain Deletion Mutant of Ebola VP40: A Role for ALIX in Ebola Virus Egress.. The Journal of infectious diseases, (1): [PMID:25786915]
38. Khan, Amjad and 6 more authors.. (2020) PDCD6IP, encoding a regulator of the ESCRT complex, is mutated in microcephaly.. Clinical genetics, [PMID:32286682]
39. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460]

Solution Calculators

Molarity Calculator

Determine the necessary mass, volume, or concentration for preparing a solution.

Dilution Calculator

Determine the dilution needed to prepare a stock solution.

SKU	Size	Availability	Price
Ab088156-10μl	10μl	In stock	$69.90
Ab088156-50μl	50μl	In stock	$199.90
Ab088156-100μl	100μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$299.90
Ab088156-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$2,699.90

Recombinant ALIX Antibody - Primary antibody, specific to PDCD6IP, Rabbit IgG