Recombinant; Rabbit anti Human Alpha Skeletal Muscle Actin Antibody; WB, IHC, ICC, IF; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab088433-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $49.90 | |
| Ab088433-50μl | 50μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $139.90 | |
| Ab088433-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $219.90 | |
| Ab088433-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,099.90 |
Recombinant; Rabbit anti Human Alpha Skeletal Muscle Actin Antibody; WB, IHC, ICC, IF; Unconjugated
| Product Name | Recombinant alpha Skeletal Muscle Actin Antibody - Primary antibody, specific to ACTA1, IgG |
|---|---|
| Synonyms | a actin antibody; ACTA antibody; ACTA1 antibody; Actin, alpha skeletal muscle antibody; ACTS_HUMAN antibody; Alpha actin 1 antibody; Alpha-actin-1 antibody; ASMA antibody; MPFD antibody; Skeletal muscle alpha actin 1 antibody; ACTA1; ACTA; Actin; alpha sk |
| Specifications & Purity | Recombinant, ExactAb™, Validated, High performance, Lot by Lot |
| Biochemical and Physiological Mechanisms | Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. |
| Host species | Rabbit |
| Specificity | Alpha Skeletal Muscle Actin |
| Immunogen | A synthetic peptide derived from human Alpha Skeletal Muscle Actin (AA 40-85). |
| Positive Control | WB: Mouse spleen, Rat heart, HeLa, A431, HEK293, NIH/3T3 and C6 lysates. IHC: Mouse heart tissue. IF/ICC: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, High Performance, Recombinant, Validated |
| Product Description |
Rabbit anti Human Alpha Skeletal Muscle Actin Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on.
|
| Isotype | Rabbit IgG |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant Alpha Skeletal Muscle Actin Antibody (Ab088433) - Western Blot
All lanes: Recombinant Alpha Skeletal Muscle Actin Antibody (Ab088433) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 3.8 s
Recombinant Alpha Skeletal Muscle Actin Antibody (Ab088433) - IF/ICC
Immunofluorescent analysis of HeLa cells using Recombinant Alpha Skeletal Muscle Actin Antibody (Ab088433).
Recombinant Alpha Skeletal Muscle Actin Antibody (Ab088433) - IF/ICC
Immunofluorescent analysis of HeLa cells using Recombinant Alpha Skeletal Muscle Actin Antibody (Ab088433) at 1/50 dilution.
Recombinant Alpha Skeletal Muscle Actin Antibody (Ab088433) - IF/ICC
Immunofluorescent analysis of HeLa cells using Recombinant Alpha Skeletal Muscle Actin Antibody (Ab088433) at 1/50 dilution.
Recombinant Alpha Skeletal Muscle Actin Antibody (Ab088433) - IHC
Immunohistochemical analysis of paraffin-embedded mouse heart, using Recombinant Alpha Skeletal Muscle Actin Antibody (Ab088433).
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| IHC | 1/100-1/500 |
| WB |
1/1000 - 1/10000
|
| IF/ICC |
1/50-1/500
|
Enter Lot Number to search for COA:
| 1. Taylor, A A, Erba, H P HP, Muscat, G E GE and Kedes, L L.. (1988) Nucleotide sequence and expression of the human skeletal alpha-actin gene: evolution of functional regulatory domains.. Genomics, [PMID:2907503] |
| 2. Hanauer, A A and 5 more authors.. (1983) Isolation and characterization of cDNA clones for human skeletal muscle alpha actin.. Nucleic acids research, (11): [PMID:6190133] |
| 3. Nowak, K J KJ and 24 more authors.. (1999) Mutations in the skeletal muscle alpha-actin gene in patients with actin myopathy and nemaline myopathy.. Nature genetics, [PMID:10508519] |
| 4. Hauser, M A MA and 17 more authors.. (2000) Myotilin is mutated in limb girdle muscular dystrophy 1A.. Human molecular genetics, (1): [PMID:10958653] |
| 5. Jungbluth, H H and 10 more authors.. (2001) Mild phenotype of nemaline myopathy with sleep hypoventilation due to a mutation in the skeletal muscle alpha-actin (ACTA1) gene.. Neuromuscular disorders : NMD, [PMID:11166164] |
| 6. Ilkovski, B B and 15 more authors.. (2001) Nemaline myopathy caused by mutations in the muscle alpha-skeletal-actin gene.. American journal of human genetics, [PMID:11333380] |
| 7. Sparrow, John C JC and 7 more authors.. (2003) Muscle disease caused by mutations in the skeletal muscle alpha-actin gene (ACTA1).. Neuromuscular disorders : NMD, [PMID:12921789] |
| 8. Ilkovski, Biljana B and 8 more authors.. (2004) Evidence for a dominant-negative effect in ACTA1 nemaline myopathy caused by abnormal folding, aggregation and altered polymerization of mutant actin isoforms.. Human molecular genetics, (15): [PMID:15198992] |
| 9. Agrawal, Pankaj B PB and 11 more authors.. (2004) Heterogeneity of nemaline myopathy cases with skeletal muscle alpha-actin gene mutations.. Annals of neurology, [PMID:15236405] |
| 10. Ohlsson, M M, Tajsharghi, H H, Darin, N N, Kyllerman, M M and Oldfors, A A.. (2004) Follow-up of nemaline myopathy in two patients with novel mutations in the skeletal muscle alpha-actin gene (ACTA1).. Neuromuscular disorders : NMD, [PMID:15336687] |
| 11. Laing, Nigel G NG and 12 more authors.. (2004) Actin mutations are one cause of congenital fibre type disproportion.. Annals of neurology, [PMID:15468086] |
| 12. Kaindl, A M AM and 11 more authors.. (2004) Missense mutations of ACTA1 cause dominant congenital myopathy with cores.. Journal of medical genetics, [PMID:15520409] |
| 13. Hutchinson, David O DO, Charlton, Amanda A, Laing, Nigel G NG, Ilkovski, Biljana B and North, Kathryn N KN.. (2006) Autosomal dominant nemaline myopathy with intranuclear rods due to mutation of the skeletal muscle ACTA1 gene: clinical and pathological variability within a kindred.. Neuromuscular disorders : NMD, [PMID:16427282] |
| 14. Bosch-Comas, A A, Lindsten, K K, Gonzàlez-Duarte, R R, Masucci, M G MG and Marfany, G G.. (2006) The ubiquitin-specific protease USP25 interacts with three sarcomeric proteins.. Cellular and molecular life sciences : CMLS, [PMID:16501887] |
| 15. Gregory, S G SG and 178 more authors.. (2006) The DNA sequence and biological annotation of human chromosome 1.. Nature, (18): [PMID:16710414] |
| 16. D'Amico, Adele A and 14 more authors.. (2006) Fatal hypertrophic cardiomyopathy and nemaline myopathy associated with ACTA1 K336E mutation.. Neuromuscular disorders : NMD, [PMID:16945537] |
| 17. Clarke, Nigel F NF and 8 more authors.. (2007) The pathogenesis of ACTA1-related congenital fiber type disproportion.. Annals of neurology, [PMID:17387733] |
| 18. Domazetovska, Ana A and 10 more authors.. (2007) Intranuclear rod myopathy: molecular pathogenesis and mechanisms of weakness.. Annals of neurology, [PMID:17705262] |
| 19. Kudryashov, Dmitri S DS and 10 more authors.. (2008) Connecting actin monomers by iso-peptide bond is a toxicity mechanism of the Vibrio cholerae MARTX toxin.. Proceedings of the National Academy of Sciences of the United States of America, (25): [PMID:19015515] |
| 20. Peng, Chao C and 18 more authors.. (2011) The first identification of lysine malonylation substrates and its regulatory enzyme.. Molecular & cellular proteomics : MCP, [PMID:21908771] |
| 21. Jain, R K RK and 14 more authors.. (2012) Nemaline myopathy with stiffness and hypertonia associated with an ACTA1 mutation.. Neurology, (3): [PMID:22442437] |
| 22. Gatayama, Ryohei R and 6 more authors.. (2013) Nemaline myopathy with dilated cardiomyopathy in childhood.. Pediatrics, [PMID:23650303] |
| 23. Li, Ming-Ming MM and 18 more authors.. (2013) ALKBH4-dependent demethylation of actin regulates actomyosin dynamics.. Nature communications, [PMID:23673617] |
| 24. Chae, Jong Hee JH and 6 more authors.. (2015) Utility of next generation sequencing in genetic diagnosis of early onset neuromuscular disorders.. Journal of medical genetics, [PMID:25635128] |
| 25. Zukosky, Kristen K and 22 more authors.. (2015) Association of a Novel ACTA1 Mutation With a Dominant Progressive Scapuloperoneal Myopathy in an Extended Family.. JAMA neurology, [PMID:25938801] |
| 26. Heisler, David B and 10 more authors.. (2015) ACTIN-DIRECTED TOXIN. ACD toxin-produced actin oligomers poison formin-controlled actin polymerization.. Science (New York, N.Y.), (31): [PMID:26228148] |
| 27. Ahmed, Atif A AA and 7 more authors.. (2018) Arthrogryposis and pterygia as lethal end manifestations of genetically defined congenital myopathies.. American journal of medical genetics. Part A, [PMID:29274205] |
| 28. Wilkinson, Alex W AW and 21 more authors.. (2019) SETD3 is an actin histidine methyltransferase that prevents primary dystocia.. Nature, [PMID:30626964] |
