Recombinant; Rabbit anti Human Anterior Gradient 2 Antibody; WB, IHC, ICC, IF; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab089031-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $66.90 | |
| Ab089031-50μl | 50μl | In stock | $189.90 | |
| Ab089031-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $289.90 | |
| Ab089031-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,599.90 |
Recombinant; Rabbit anti Human Anterior Gradient 2 Antibody; WB, IHC, ICC, IF; Unconjugated
| Product Name | Recombinant Anterior Gradient 2 Antibody - Primary antibody, specific to Anterior Gradient 2, Rabbit IgG |
|---|---|
| Synonyms | AG 2 antibody; AG 2 protein antibody; AG-2 antibody; AG2 antibody; AG2 protein antibody; AGR 2 antibody; AGR2 antibody; AGR2_HUMAN antibody; Anterior gradient 2 homolog (Xenopus laevis) antibody; anterior gradient 2 homolog antibody; Anterior gradient 2, |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.4 mg/mL |
| Host species | Rabbit |
| Specificity | Anterior Gradient 2 |
| Immunogen | A synthetic peptide derived from human Anterior Gradient 2 (AA 27-40) |
| Positive Control | WB: K562, C6 and NIH/3T3 cell lysates. IHC: Colon tissue. ICC/IF: MCF7 cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description |
Rabbit anti Human Anterior Gradient 2 Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purification |
| Form | Liquid |
| Concentration | 0.4 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant Anterior Gradient 2 Antibody (Ab089031) - Western Blot
All lanes: Recombinant Anterior Gradient 2 Antibody (Ab089031) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 20 kDa
Observed band size: 16 kDa
Exposure time: 25.6 s
Recombinant Anterior Gradient 2 Antibody (Ab089031) - IF/ICC
Immunocytochemistry analysis of Anterior Gradient 2 (green) in MCF7 using Recombinant Anterior Gradient 2 Antibody (Ab089031), and cells were counterstained with DAPI (blue).
Recombinant Anterior Gradient 2 Antibody (Ab089031) - IHC
Immunohistochemistry analysis of paraffin-embedded colon using Recombinant Anterior Gradient 2 Antibody (Ab089031). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
| ICC/IF |
1/50-1/200
|
| IHC | 1/50 - 1/100 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F1202246 | Certificate of Analysis | Dec 26, 2023 | Ab089031 |
| ZJ23F1202245 | Certificate of Analysis | Dec 26, 2023 | Ab089031 |
| 1. Clark HF, Gurney AL, Abaya E, Baker K, Baldwin D, Brush J, Chen J, Chow B, Chui C, Crowley C et al.. (2003) The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment.. Genome Res, 13 (10): (2265-70). [PMID:12975309] |
| 2. Thompson, D A DA and Weigel, R J RJ.. (1998) hAG-2, the human homologue of the Xenopus laevis cement gland gene XAG-2, is coexpressed with estrogen receptor in breast cancer cell lines.. Biochemical and biophysical research communications, (9): [PMID:9790916] |
| 3. Fletcher, G C GC and 10 more authors.. (2003) hAG-2 and hAG-3, human homologues of genes involved in differentiation, are associated with oestrogen receptor-positive breast tumours and interact with metastasis gene C4.4a and dystroglycan.. British journal of cancer, (24): [PMID:12592373] |
| 4. Hillier, Ladeana W LW and 106 more authors.. (2003) The DNA sequence of human chromosome 7.. Nature, (10): [PMID:12853948] |
| 5. Zhang, Zemin Z and Henzel, William J WJ.. (2004) Signal peptide prediction based on analysis of experimentally verified cleavage sites.. Protein science : a publication of the Protein Society, [PMID:15340161] |
| 6. Zhang, Jin-San JS, Gong, Aiyu A, Cheville, John C JC, Smith, David I DI and Young, Charles Y F CY.. (2005) AGR2, an androgen-inducible secretory protein overexpressed in prostate cancer.. Genes, chromosomes & cancer, [PMID:15834940] |
| 7. Wang, Zheng Z, Hao, Ying Y and Lowe, Anson W AW.. (2008) The adenocarcinoma-associated antigen, AGR2, promotes tumor growth, cell migration, and cellular transformation.. Cancer research, (15): [PMID:18199544] |
| 8. Park, Sung-Woo SW and 7 more authors.. (2009) The protein disulfide isomerase AGR2 is essential for production of intestinal mucus.. Proceedings of the National Academy of Sciences of the United States of America, (28): [PMID:19359471] |
| 9. Patel, Pryank P and 6 more authors.. (2013) Metastasis-promoting anterior gradient 2 protein has a dimeric thioredoxin fold structure and a role in cell adhesion.. Journal of molecular biology, (11): [PMID:23274113] |
