A synthetic peptide derived from human AP3M1 (AA 146-159)
Positive Control
WB: HEK293, HeLa, K562, NIH/3T3 and C6 cell lysates.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human AP3M1 Antibody, Recombinant, could be used for WB and so on. Application: WB: 1/500-1/1000 Protein Function Part of the AP-3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes.
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Form
Liquid
Concentration
0.3 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant AP3M1 Antibody (Ab089101) - Western Blot All lanes: Recombinant AP3M1 Antibody (Ab089101) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 47 kDa Observed band size: 44 kDa Exposure time: 51.9 s
1.Dell'Angelica, E C EC, Shotelersuk, V V, Aguilar, R C RC, Gahl, W A WA and Bonifacino, J S JS.. (1999) Altered trafficking of lysosomal proteins in Hermansky-Pudlak syndrome due to mutations in the beta 3A subunit of the AP-3 adaptor.. Molecular cell, [PMID:10024875]
2.Deloukas, P P and 135 more authors.. (2004) The DNA sequence and comparative analysis of human chromosome 10.. Nature, (27):[PMID:15164054]
3.Ward, Casey; Maselko, Maciej; Lupfer, Christopher; Prescott, Meagan and Pastey, Manoj K.. (2017) Interaction of the Human Respiratory Syncytial Virus matrix protein with cellular adaptor protein complex 3 plays a critical role in trafficking.. PloS one, [PMID:29028839]
4.Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460]
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