Basic Description

Product Name	Recombinant APG5L/ATG5 Antibody - Primary antibody, specific to ATG5, Rabbit IgG
Synonyms	APG 5 antibody \| APG 5L antibody \| APG5 antibody \| APG5 autophagy 5 like antibody \| APG5 like antibody \| APG5-like antibody \| APG5L antibody \| Apoptosis specific proteinantibody \| Apoptosis-specific protein antibody \| ASP antibody \| ATG 5 antibody \| Atg5
Specifications & Purity	ExactAb™, Validated, Recombinant, 0.3 mg/mL
Host species	Rabbit
Specificity	ATG5
Immunogen	A synthetic peptide derived from human APG5L/ATG5 (AA 21-34)
Positive Control	WB: HeLa, K562, A431, THP-1, HUVEC, NIH/3T3 and C6 cell lysates. ICC/IF: MCF7 cells.
Conjugation	Unconjugated
Grade	ExactAb™, Recombinant, Validated
Product Description	Rabbit anti Human APG5L/ATG5 Antibody, Recombinant, could be used for WB, ICC, IF and so on. Application: WB: 1/500-1/1000 ICC/IF: 1/50-1/200 Protein Function: Involved in autophagic vesicle formation. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity. The ATG12-ATG5 conjugate also negatively regulates the innate antiviral immune response by blocking the type I IFN production pathway through direct association with RARRES3 and MAVS. Also plays a role in translation or delivery of incoming viral RNA to the translation apparatus. Plays a critical role in multiple aspects of lymphocyte development and is essential for both B and T lymphocyte survival and proliferation. Required for optimal processing and presentation of antigens for MHC II. Involved in the maintenance of axon morphology and membrane structures, as well as in normal adipocyte differentiation. Promotes primary ciliogenesis through removal of OFD1 from centriolar satellites and degradation of IFT20 via the autophagic pathway. May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Plays a crucial role in IFN-gamma-induced autophagic cell death by interacting with FADD.

Product Properties

Isotype	Rabbit IgG
Light Chain Type	kappa
SDS-PAGE	150kDa
Purification Method	Antigen affinity purifie
Form	Liquid
Concentration	0.3 mg/mL
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant APG5L/ATG5 Antibody ( Ab089177) - Western Blot
All lanes: Recombinant APG5L/ATG5 Antibody (Ab089177) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 32 kDa
Observed band size: 51 kDa
Exposure time: 4.9 s

Recombinant APG5L/ATG5 Antibody ( Ab089177) - IF/ICC
Immunocytochemistry analysis of APG5L/ATG5 (green) in MCF7 using Recombinant APG5L/ATG5 Antibody (Ab089177) and DAPI (blue).

Associated Targets(Human)

ATG5 Tbio Autophagy protein 5 (0 Activities)

Discover Target: ATG5

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Application

Application	Dilution info
WB	1/500-1/1000 "> 1/500-1/1000

Certificates

Certificate of Analysis(COA)

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2 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0809227	Certificate of Analysis	Aug 05, 2024	Ab089177
ZJ24F0809226	Certificate of Analysis	Aug 05, 2024	Ab089177

FAQs and Articles

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Protocol of Western Blotting For High Molecular Weights

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Western blotting Biological buffer Immunoblotting Protein Electrophoresis High Molecular Weights large molecular proteins WB antibody

References

1. Grand, R J RJ and 6 more authors.. (1995) A novel protein expressed in mammalian cells undergoing apoptosis.. Experimental cell research, [PMID:7796880]
2. Hammond, E M EM and 7 more authors.. (1998) Homology between a human apoptosis specific protein and the product of APG5, a gene involved in autophagy in yeast.. FEBS letters, (3): [PMID:9563500]
3. Mizushima, N N, Sugita, H H, Yoshimori, T T and Ohsumi, Y Y.. (1998) A new protein conjugation system in human. The counterpart of the yeast Apg12p conjugation system essential for autophagy.. The Journal of biological chemistry, (18): [PMID:9852036]
4. Tanida, I I, Tanida-Miyake, E E, Ueno, T T and Kominami, E E.. (2001) The human homolog of Saccharomyces cerevisiae Apg7p is a Protein-activating enzyme for multiple substrates including human Apg12p, GATE-16, GABARAP, and MAP-LC3.. The Journal of biological chemistry, (19): [PMID:11096062]
5. Mizushima, N N and 8 more authors.. (2001) Dissection of autophagosome formation using Apg5-deficient mouse embryonic stem cells.. The Journal of cell biology, (19): [PMID:11266458]
6. Tanida, Isei I, Tanida-Miyake, Emiko E, Komatsu, Masaaki M, Ueno, Takashi T and Kominami, Eiki E.. (2002) Human Apg3p/Aut1p homologue is an authentic E2 enzyme for multiple substrates, GATE-16, GABARAP, and MAP-LC3, and facilitates the conjugation of hApg12p to hApg5p.. The Journal of biological chemistry, (19): [PMID:11825910]
7. Tanida, Isei I, Nishitani, Tomohito T, Nemoto, Takahiro T, Ueno, Takashi T and Kominami, Eiki E.. (2002) Mammalian Apg12p, but not the Apg12p.Apg5p conjugate, facilitates LC3 processing.. Biochemical and biophysical research communications, (6): [PMID:12207896]
8. Pyo, Jong-Ok JO and 12 more authors.. (2005) Essential roles of Atg5 and FADD in autophagic cell death: dissection of autophagic cell death into vacuole formation and cell death.. The Journal of biological chemistry, (27): [PMID:15778222]
9. Sjöblom, Tobias T and 28 more authors.. (2006) The consensus coding sequences of human breast and colorectal cancers.. Science (New York, N.Y.), (13): [PMID:16959974]
10. Shao, Yufang Y, Gao, Zhonghua Z, Feldman, Taya T and Jiang, Xuejun X.. () Stimulation of ATG12-ATG5 conjugation by ribonucleic acid.. Autophagy, [PMID:16963840]
11. Jounai, Nao N and 10 more authors.. (2007) The Atg5 Atg12 conjugate associates with innate antiviral immune responses.. Proceedings of the National Academy of Sciences of the United States of America, (28): [PMID:17709747]
12. Bechtel, Stephanie S and 18 more authors.. (2007) The full-ORF clone resource of the German cDNA Consortium.. BMC genomics, (31): [PMID:17974005]
13. Lee, In Hye IH and Finkel, Toren T.. (2009) Regulation of autophagy by the p300 acetyltransferase.. The Journal of biological chemistry, (6): [PMID:19124466]
14. Guévin, Carl C and 5 more authors.. (2010) Autophagy protein ATG5 interacts transiently with the hepatitis C virus RNA polymerase (NS5B) early during infection.. Virology, (15): [PMID:20580051]
15. Ogawa, Michinaga M and 17 more authors.. (2011) A Tecpr1-dependent selective autophagy pathway targets bacterial pathogens.. Cell host & microbe, (19): [PMID:21575909]
16. Mai, Sören S, Muster, Britta B, Bereiter-Hahn, Jürgen J and Jendrach, Marina M.. (2012) Autophagy proteins LC3B, ATG5 and ATG12 participate in quality control after mitochondrial damage and influence lifespan.. Autophagy, [PMID:22170153]
17. Chen, Dandan D and 5 more authors.. (2012) A mammalian autophagosome maturation mechanism mediated by TECPR1 and the Atg12-Atg5 conjugate.. Molecular cell, (9): [PMID:22342342]
18. Van Damme, Petra P and 15 more authors.. (2012) N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.. Proceedings of the National Academy of Sciences of the United States of America, (31): [PMID:22814378]
19. Otomo, Chinatsu C, Metlagel, Zoltan Z, Takaesu, Giichi G and Otomo, Takanori T.. (2013) Structure of the human ATG12~ATG5 conjugate required for LC3 lipidation in autophagy.. Nature structural & molecular biology, [PMID:23202584]
20. Kim, Myungjin M and 21 more authors.. (2016) Mutation in ATG5 reduces autophagy and leads to ataxia with developmental delay.. eLife, (26): [PMID:26812546]
21. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460]

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Recombinant APG5L/ATG5 Antibody - Primary antibody, specific to ATG5, Rabbit IgG