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Recombinant APPBP1 Antibody - Primary antibody, specific to NAE1, Rabbit IgG

  • ExactAb™
  • Recombinant
  • Validated
  • 0.6 mg/mL
Features and benefits
  • Short Overview:

    Recombinant; Rabbit anti Human APPBP1 Antibody; WB; Unconjugated

  • Species reactivity(Reacts with): Human
  • Isotype: Rabbit IgG
    Application:
  • WB
Item Number
Ab089387
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab089387-10μl
10μl
In stock
$69.90
Ab089387-50μl
50μl
In stock
$199.90
Ab089387-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$309.90
Ab089387-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,999.90

Recombinant; Rabbit anti Human APPBP1 Antibody; WB; Unconjugated

Basic Description

Product NameRecombinant APPBP1 Antibody - Primary antibody, specific to NAE1, Rabbit IgG
Specifications & PurityExactAb™, Validated, Recombinant, 0.6 mg/mL
Host speciesRabbit
SpecificityNAE1
ImmunogenA synthetic peptide derived from human APPBP1 (AA 61-74)
Positive ControlWB: SK-OV-3, U-2 OS, HeLa, A431 and K562 cell lysates.
ConjugationUnconjugated
GradeExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human APPBP1 Antibody, Recombinant, could be used for WB and so on.

Application:
WB: 1/500-1/1000

Protein Function:

Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation.

Product Properties

IsotypeRabbit IgG
SDS-PAGE150 kDa
Purification MethodAntigen Affinity Purified
FormLiquid
Concentration0.6 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant APPBP1 Antibody (Ab089387) - Western Blot
All lanes: Recombinant APPBP1 Antibody (Ab089387) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 60 kDa
Observed band size: 62 kDa
Exposure time: 31.7 s

Associated Targets

NAE1 Tchem NEDD8-activating enzyme E1 regulatory subunit 0 Activities

Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
WB

1/500-1/1000

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section

2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0505872Certificate of AnalysisMay 21, 2024 Ab089387
ZJ24F0505873Certificate of AnalysisMay 21, 2024 Ab089387

Related Documents

Solution Calculators