| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab089387-10μl | 10μl | In stock | $69.90 | |
| Ab089387-50μl | 50μl | In stock | $199.90 | |
| Ab089387-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $309.90 | |
| Ab089387-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,999.90 |
Recombinant; Rabbit anti Human APPBP1 Antibody; WB; Unconjugated
| Product Name | Recombinant APPBP1 Antibody - Primary antibody, specific to NAE1, Rabbit IgG |
|---|---|
| Synonyms | NAE1 | A-116A10.1 | APPBP1 | HPP1 | NEDFIH | ula-1 | 59 kDa antibody | A 116A10.1 antibody | Amyloid beta precursor protein binding protein 1 59kD antibody | Amyloid beta precursor protein binding protein 1 59kDa antibody | Amyloid beta precursor protein |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.6 mg/mL |
| Host species | Rabbit |
| Specificity | NAE1 |
| Immunogen | A synthetic peptide derived from human APPBP1 (AA 61-74) |
| Positive Control | WB: SK-OV-3, U-2 OS, HeLa, A431 and K562 cell lysates. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human APPBP1 Antibody, Recombinant, could be used for WB and so on. Application: Protein Function: Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen Affinity Purified |
| Form | Liquid |
| Concentration | 0.6 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant APPBP1 Antibody (Ab089387) - Western Blot
All lanes: Recombinant APPBP1 Antibody (Ab089387) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 60 kDa
Observed band size: 62 kDa
Exposure time: 31.7 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000"> 1/500-1/1000 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0505872 | Certificate of Analysis | May 21, 2024 | Ab089387 |
| ZJ24F0505873 | Certificate of Analysis | May 21, 2024 | Ab089387 |
