Recombinant; Rabbit anti Human APPL Antibody; WB, IHC; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab089390-10μl | 10μl | In stock | $79.90 | |
| Ab089390-50μl | 50μl | In stock | $219.90 | |
| Ab089390-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $339.90 | |
| Ab089390-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,699.90 |
Recombinant; Rabbit anti Human APPL Antibody; WB, IHC; Unconjugated
| Product Name | Recombinant APPL Antibody - Primary antibody, specific to APPL1, Rabbit IgG |
|---|---|
| Synonyms | Adapter protein containing PH domain antibody | Adaptor Protein Containing pH Domain antibody | Adaptor protein containing pH domain PTB domain and leucine zipper motif 1 antibody | Adaptor protein containing pH domain PTB domain and leucine zipper motif |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.3 mg/mL |
| Host species | Rabbit |
| Specificity | APPL1 |
| Immunogen | A synthetic peptide derived from human APPL (AA 402-413) |
| Positive Control | WB: HeLa, HEK293, MCF7 and NIH/3T3 cell lysates. IHC: Human lung tissue. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description |
Rabbit anti Human APPL Antibody, Recombinant, could be used for WB, IHC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified |
| Form | Liquid |
| Concentration | 0.3 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant APPL Antibody (Ab089390) - Western Blot
All lanes: Recombinant APPL Antibody (Ab089390) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 80 kDa
Observed band size: 87 kDa
Exposure time: 60.0 s
Recombinant APPL Antibody (Ab089390) - IHC
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant APPL Antibody (Ab089390). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
| IHC | 1/50 - 1/100 |
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0303793 | Certificate of Analysis | Mar 27, 2024 | Ab089390 |
| ZJ24F0303792 | Certificate of Analysis | Mar 27, 2024 | Ab089390 |
| 1. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330] |
| 2. Mitsuuchi, Y Y and 5 more authors.. (1999) Identification of a chromosome 3p14.3-21.1 gene, APPL, encoding an adaptor molecule that interacts with the oncoprotein-serine/threonine kinase AKT2.. Oncogene, (2): [PMID:10490823] |
| 3. Nagase, T T, Kikuno, R R, Ishikawa, K I KI, Hirosawa, M M and Ohara, O O.. (2000) Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro.. DNA research : an international journal for rapid publication of reports on genes and genomes, (28): [PMID:10718198] |
| 4. Liu, Jiayou J and 6 more authors.. (2002) Mediation of the DCC apoptotic signal by DIP13 alpha.. The Journal of biological chemistry, (19): [PMID:12011067] |
| 5. Nakajima, Daisuke D and 5 more authors.. (2002) Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones.. DNA research : an international journal for rapid publication of reports on genes and genomes, (30): [PMID:12168954] |
| 6. Miaczynska, Marta M and 8 more authors.. (2004) APPL proteins link Rab5 to nuclear signal transduction via an endosomal compartment.. Cell, (6): [PMID:15016378] |
| 7. Sjöblom, Tobias T and 28 more authors.. (2006) The consensus coding sequences of human breast and colorectal cancers.. Science (New York, N.Y.), (13): [PMID:16959974] |
| 8. Nechamen, Cheryl A CA, Thomas, Richard M RM and Dias, James A JA.. (2007) APPL1, APPL2, Akt2 and FOXO1a interact with FSHR in a potential signaling complex.. Molecular and cellular endocrinology, (2): [PMID:17030088] |
| 9. Matsuoka, Shuhei S and 12 more authors.. (2007) ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage.. Science (New York, N.Y.), (25): [PMID:17525332] |
| 10. Erdmann, Kai S KS and 9 more authors.. (2007) A role of the Lowe syndrome protein OCRL in early steps of the endocytic pathway.. Developmental cell, [PMID:17765681] |
| 11. Chial, Heidi J HJ and 5 more authors.. (2008) Membrane targeting by APPL1 and APPL2: dynamic scaffolds that oligomerize and bind phosphoinositides.. Traffic (Copenhagen, Denmark), [PMID:18034774] |
| 12. Dephoure, Noah N and 6 more authors.. (2008) A quantitative atlas of mitotic phosphorylation.. Proceedings of the National Academy of Sciences of the United States of America, (5): [PMID:18669648] |
| 13. Rashid, Sajid S and 5 more authors.. (2009) Endosomal adaptor proteins APPL1 and APPL2 are novel activators of beta-catenin/TCF-mediated transcription.. The Journal of biological chemistry, (3): [PMID:19433865] |
| 14. Wang, Changhua C and 10 more authors.. (2009) Yin-Yang regulation of adiponectin signaling by APPL isoforms in muscle cells.. The Journal of biological chemistry, (13): [PMID:19661063] |
| 15. Swan, Laura E LE, Tomasini, Livia L, Pirruccello, Michelle M, Lunardi, Joël J and De Camilli, Pietro P.. (2010) Two closely related endocytic proteins that share a common OCRL-binding motif with APPL1.. Proceedings of the National Academy of Sciences of the United States of America, (23): [PMID:20133602] |
| 16. Noakes, Christopher J CJ, Lee, Grace G and Lowe, Martin M.. (2011) The PH domain proteins IPIP27A and B link OCRL1 to receptor recycling in the endocytic pathway.. Molecular biology of the cell, (1): [PMID:21233288] |
| 17. Urbanska, Anna A, Sadowski, Lukasz L, Kalaidzidis, Yannis Y and Miaczynska, Marta M.. (2011) Biochemical characterization of APPL endosomes: the role of annexin A2 in APPL membrane recruitment.. Traffic (Copenhagen, Denmark), [PMID:21645192] |
| 18. Pirruccello, Michelle M, Swan, Laura E LE, Folta-Stogniew, Ewa E and De Camilli, Pietro P.. (2011) Recognition of the F&H motif by the Lowe syndrome protein OCRL.. Nature structural & molecular biology, (12): [PMID:21666675] |
| 19. Van Damme, Petra P and 15 more authors.. (2012) N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.. Proceedings of the National Academy of Sciences of the United States of America, (31): [PMID:22814378] |
| 20. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
| 21. Bian, Yangyang Y and 9 more authors.. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.. Journal of proteomics, (16): [PMID:24275569] |
| 22. Cheng, Kenneth K Y KK and 8 more authors.. (2014) The adaptor protein APPL2 inhibits insulin-stimulated glucose uptake by interacting with TBC1D1 in skeletal muscle.. Diabetes, [PMID:24879834] |
| 23. Prudente, Sabrina S and 23 more authors.. (2015) Loss-of-Function Mutations in APPL1 in Familial Diabetes Mellitus.. American journal of human genetics, (2): [PMID:26073777] |
| 24. Song, Jie J and 6 more authors.. (2016) APPL proteins promote TGFβ-induced nuclear transport of the TGFβ type I receptor intracellular domain.. Oncotarget, (5): [PMID:26583432] |
