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Size

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Ab089519-10μl

10μl

Available within 8-12 weeks(?)

$66.90

Ab089519-50μl

50μl

Available within 4-8 weeks(?)

$189.90

Ab089519-100μl

100μl

Available within 4-8 weeks(?)

$299.90

Ab089519-1ml

1ml

Available within 8-12 weeks(?)

$1,799.90

Basic Description

Product Name	Recombinant Argonaute-2 Antibody - Primary antibody, specific to AGO2, Rabbit IgG
Synonyms	Ago 2 antibody; AGO2_HUMAN antibody; Argonaute 2 antibody; argonaute 2, RISC catalytic component antibody; Argonaute RISC catalytic component 2 antibody; Argonaute2 antibody; CTA-204B4.6 antibody; dAgo2 antibody; eIF 2C 2 antibody; eIF-2C 2 antibody; eIF2
Specifications & Purity	ExactAb™, Validated, Recombinant, 0.5 mg/mL
Host species	Rabbit
Specificity	AGO2
Immunogen	A synthetic peptide derived from human Argonaute-2 (AA 381-396).
Positive Control	WB: K562, MCF-7, HeLa, Hep G2 and A431 cell lysates. ICC/IF: HCT116 cells.
Conjugation	Unconjugated
Grade	ExactAb™, Recombinant, Validated
Product Description	Rabbit anti Human Argonaute-2 Antibody, Recombinant, could be used for WB, IF, ICC and so on. Application WB: 1/500-1/1000 IF/ICC: 1/50-1/200 Protein Function Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include EIF2C2/AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by EIF2C2/AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'-UTR). Can also upregulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the 3'-UTR of the TNF (TNF-alpha) mRNA and upregulates translation under conditions of serum starvation. Also required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct the transcriptional repression of complementary promoter regions.

Product Properties

Isotype	Rabbit IgG
SDS-PAGE	150 kDa
Purification Method	Antigen affinity purification
Form	Liquid
Concentration	0.5 mg/mL
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant Argonaute-2 Antibody (Ab089519) - Western Blot
All lanes: Recombinant Argonaute-2 Antibody (Ab089519) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 97 kDa
Observed band size: 90 kDa
Exposure time: 20.0 s

Recombinant Argonaute-2 Antibody (Ab089519) - IF
Immunocytochemistry analysis of Argonaute 2 (green) in HCT116 cells using Recombinant Argonaute-2 Antibody (Ab089519) and cells were counterstained with DAPI (blue).

Associated Targets

AGO2 Tbio Protein argonaute-2 0 Activities

Discover Target: AGO2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Application

Application	Dilution info
WB	1/500-1/1000
IF/ICC	1/50 - 1/200

Certificates

Certificate of Analysis(COA)

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2 results found

Lot Number	Certificate Type	Date	Item
ZJ23F0901092	Certificate of Analysis	Sep 18, 2023	Ab089519
ZJ23F0901093	Certificate of Analysis	Sep 18, 2023	Ab089519

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References

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13. Haase, Astrid D AD and 6 more authors.. (2005) TRBP, a regulator of cellular PKR and HIV-1 virus expression, interacts with Dicer and functions in RNA silencing.. EMBO reports, [PMID:16142218]
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16. Nusbaum, Chad C and 75 more authors.. (2006) DNA sequence and analysis of human chromosome 8.. Nature, (19): [PMID:16421571]
17. Wichroski, Michael J MJ, Robb, G Brett GB and Rana, Tariq M TM.. (2006) Human retroviral host restriction factors APOBEC3G and APOBEC3F localize to mRNA processing bodies.. PLoS pathogens, [PMID:16699599]
18. Chu, Chia-ying CY and Rana, Tariq M TM.. (2006) Translation repression in human cells by microRNA-induced gene silencing requires RCK/p54.. PLoS biology, [PMID:16756390]
19. Janowski, Bethany A BA and 7 more authors.. (2006) Involvement of AGO1 and AGO2 in mammalian transcriptional silencing.. Nature structural & molecular biology, [PMID:16936728]
20. Vasudevan, Shobha S and Steitz, Joan A JA.. (2007) AU-rich-element-mediated upregulation of translation by FXR1 and Argonaute 2.. Cell, (23): [PMID:17382880]
21. Chendrimada, Thimmaiah P TP and 7 more authors.. (2007) MicroRNA silencing through RISC recruitment of eIF6.. Nature, (14): [PMID:17507929]
22. Kiriakidou, Marianthi M and 5 more authors.. (2007) An mRNA m7G cap binding-like motif within human Ago2 represses translation.. Cell, (15): [PMID:17524464]
23. Robb, G Brett GB and Rana, Tariq M TM.. (2007) RNA helicase A interacts with RISC in human cells and functions in RISC loading.. Molecular cell, (25): [PMID:17531811]
24. Höck, Julia J and 7 more authors.. (2007) Proteomic and functional analysis of Argonaute-containing mRNA-protein complexes in human cells.. EMBO reports, [PMID:17932509]
25. Vasudevan, Shobha S, Tong, Yingchun Y and Steitz, Joan A JA.. (2007) Switching from repression to activation: microRNAs can up-regulate translation.. Science (New York, N.Y.), (21): [PMID:18048652]
26. MacRae, Ian J IJ, Ma, Enbo E, Zhou, Min M, Robinson, Carol V CV and Doudna, Jennifer A JA.. (2008) In vitro reconstitution of the human RISC-loading complex.. Proceedings of the National Academy of Sciences of the United States of America, (15): [PMID:18178619]
27. Wu, Ligang L, Fan, Jihua J and Belasco, Joel G JG.. (2008) Importance of translation and nonnucleolytic ago proteins for on-target RNA interference.. Current biology : CB, (9): [PMID:18771919]
28. Weinmann, Lasse L and 9 more authors.. (2009) Importin 8 is a gene silencing factor that targets argonaute proteins to distinct mRNAs.. Cell, (6): [PMID:19167051]
29. Lin, Jung-Chun JC and Tarn, Woan-Yuh WY.. (2009) RNA-binding motif protein 4 translocates to cytoplasmic granules and suppresses translation via argonaute2 during muscle cell differentiation.. The Journal of biological chemistry, (11): [PMID:19801630]
30. Frank, Filipp F, Sonenberg, Nahum N and Nagar, Bhushan B.. (2010) Structural basis for 5'-nucleotide base-specific recognition of guide RNA by human AGO2.. Nature, (10): [PMID:20505670]
31. James, Victoria V and 14 more authors.. (2010) LIM-domain proteins, LIMD1, Ajuba, and WTIP are required for microRNA-mediated gene silencing.. Proceedings of the National Academy of Sciences of the United States of America, (13): [PMID:20616046]
32. Braun, Joerg E JE, Huntzinger, Eric E, Fauser, Maria M and Izaurralde, Elisa E.. (2011) GW182 proteins directly recruit cytoplasmic deadenylase complexes to miRNA targets.. Molecular cell, (7): [PMID:21981923]
33. Schirle, Nicole T NT and MacRae, Ian J IJ.. (2012) The crystal structure of human Argonaute2.. Science (New York, N.Y.), (25): [PMID:22539551]
34. Elkayam, Elad E and 6 more authors.. (2012) The structure of human argonaute-2 in complex with miR-20a.. Cell, (6): [PMID:22682761]
35. Liu, Chao C and 8 more authors.. (2012) APOBEC3G inhibits microRNA-mediated repression of translation by interfering with the interaction between Argonaute-2 and MOV10.. The Journal of biological chemistry, (24): [PMID:22791714]
36. Phalora, Prabhjeet K PK, Sherer, Nathan M NM, Wolinsky, Steven M SM, Swanson, Chad M CM and Malim, Michael H MH.. (2012) HIV-1 replication and APOBEC3 antiviral activity are not regulated by P bodies.. Journal of virology, [PMID:22915799]
37. Loedige, Inga I, Gaidatzis, Dimos D, Sack, Ragna R, Meister, Gunter G and Filipowicz, Witold W.. (2013) The mammalian TRIM-NHL protein TRIM71/LIN-41 is a repressor of mRNA function.. Nucleic acids research, (7): [PMID:23125361]
38. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163]
39. Horman, Shane R SR and 13 more authors.. (2013) Akt-mediated phosphorylation of argonaute 2 downregulates cleavage and upregulates translational repression of MicroRNA targets.. Molecular cell, (9): [PMID:23603119]
40. Faehnle, Christopher R CR, Elkayam, Elad E, Haase, Astrid D AD, Hannon, Gregory J GJ and Joshua-Tor, Leemor L.. (2013) The making of a slicer: activation of human Argonaute-1.. Cell reports, (27): [PMID:23746446]
41. Gregersen, Lea H LH and 7 more authors.. (2014) MOV10 Is a 5' to 3' RNA helicase contributing to UPF1 mRNA target degradation by translocation along 3' UTRs.. Molecular cell, (22): [PMID:24726324]
42. Srivastava, Monika M and 19 more authors.. (2015) Roquin binds microRNA-146a and Argonaute2 to regulate microRNA homeostasis.. Nature communications, (20): [PMID:25697406]
43. Golden, Ryan J RJ and 14 more authors.. (2017) An Argonaute phosphorylation cycle promotes microRNA-mediated silencing.. Nature, (9): [PMID:28114302]
44. Elbarbary, Reyad A RA and 6 more authors.. (2017) Tudor-SN-mediated endonucleolytic decay of human cell microRNAs promotes G1/S phase transition.. Science (New York, N.Y.), (26): [PMID:28546213]
45. Youn, Ji-Young JY and 14 more authors.. (2018) High-Density Proximity Mapping Reveals the Subcellular Organization of mRNA-Associated Granules and Bodies.. Molecular cell, (1): [PMID:29395067]
46. Liu, XueQiao; Sadaoka, Tomohiko; Krogmann, Tammy and Cohen, Jeffrey I.. (2020) Epstein-Barr Virus (EBV) Tegument Protein BGLF2 Suppresses Type I Interferon Signaling To Promote EBV Reactivation.. Journal of virology, (18): [PMID:32213613]

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Recombinant Argonaute-2 Antibody - Primary antibody, specific to AGO2, Rabbit IgG