Basic Description

Product Name	ARID1A Antibody - Primary antibody, specific to ARID1A, Rabbit IgG
Synonyms	actin-dependent regulator of chromatin subfamily F member 1 antibody \| ARI1A_HUMAN antibody \| ARID domain containing protein 1A antibody \| ARID domain-containing protein 1A antibody \| ARID1A antibody \| AT rich interactive domain 1A (SWI like) antibody \| A
Specifications & Purity	ExactAb™, Validated, Recombinant, Lot by Lot
Biochemical and Physiological Mechanisms	Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Binds DNA non-specifically. Also involved in vitamin D-coupled transcription regulation via its association with the WIN
Host species	Rabbit
Specificity	ARID1A
Immunogen	A synthetic peptide derived from human ARID1A (AA 1200-1400).
Positive Control	WB: A375, 293 and HeLa lysates. IHC: Human colon adenocarcinoma tissue. ICC/IF: SH-SY5Y cells.
Conjugation	Unconjugated
Grade	ExactAb™, Recombinant, Validated
Product Description	Rabbit anti Human ARID1A Antibody, Recombinant, could be used for WB, IHC, IF/ICC and so on.

Product Properties

Isotype	Rabbit IgG
Light Chain Type	kappa
SDS-PAGE	150 kDa
Purification Method	Protein A purified
Form	Liquid
Concentration	Lot by Lot
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

ARID1A Tbio AT-rich interactive domain-containing protein 1A (0 Activities)

Discover Target: ARID1A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Application

Application	Dilution info
IHC	1/100 - 1/200
WB	1/1000 - 1/2000
IF/ICC	1/50 - 1/200

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

References

1. Lemon B, Inouye C, King DS, Tjian R. (2001) Selectivity of chromatin-remodelling cofactors for ligand-activated transcription.. Nature, 414 (6866): (924-8). [PMID:11780067]
2. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M. (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.. Science, 325 (5942): (834-40). [PMID:19608861]
3. Kadoch C, Crabtree GR. (2015) Mammalian SWI/SNF chromatin remodeling complexes and cancer: Mechanistic insights gained from human genomics.. Sci Adv, 1 (5): (e1500447). [PMID:26601204]
4. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330]
5. Wang, W W and 5 more authors.. (1996) Diversity and specialization of mammalian SWI/SNF complexes.. Genes & development, (1): [PMID:8804307]
6. Takeuchi, T T, Chen, B K BK, Qiu, Y Y, Sonobe, H H and Ohtsuki, Y Y.. (1997) Molecular cloning and expression of a novel human cDNA containing CAG repeats.. Gene, (19): [PMID:9434167]
7. Dallas, P B PB and 5 more authors.. (2000) The human SWI-SNF complex protein p270 is an ARID family member with non-sequence-specific DNA binding activity.. Molecular and cellular biology, [PMID:10757798]
8. Nie, Z Z and 6 more authors.. (2000) A specificity and targeting subunit of a human SWI/SNF family-related chromatin-remodeling complex.. Molecular and cellular biology, [PMID:11073988]
9. Kozmik, Z Z and 5 more authors.. (2001) Characterization of mammalian orthologues of the Drosophila osa gene: cDNA cloning, expression, chromosomal localization, and direct physical interaction with Brahma chromatin-remodeling complex.. Genomics, (15): [PMID:11318604]
10. Kato, Hiroyuki H and 10 more authors.. (2002) SYT associates with human SNF/SWI complexes and the C-terminal region of its fusion partner SSX1 targets histones.. The Journal of biological chemistry, (15): [PMID:11734557]
11. Hurlstone, Adam F L AF, Olave, Ivan A IA, Barker, Nick N, van Noort, Mascha M and Clevers, Hans H.. (2002) Cloning and characterization of hELD/OSA1, a novel BRG1 interacting protein.. The Biochemical journal, (15): [PMID:11988099]
12. Inoue, Hiroko H and 5 more authors.. (2002) Largest subunits of the human SWI/SNF chromatin-remodeling complex promote transcriptional activation by steroid hormone receptors.. The Journal of biological chemistry, (1): [PMID:12200431]
13. Nie, Zuqin Z and 11 more authors.. (2003) Novel SWI/SNF chromatin-remodeling complexes contain a mixed-lineage leukemia chromosomal translocation partner.. Molecular and cellular biology, [PMID:12665591]
14. Martens, Joseph A JA and Winston, Fred F.. (2003) Recent advances in understanding chromatin remodeling by Swi/Snf complexes.. Current opinion in genetics & development, [PMID:12672490]
15. Kim, Suhkmann S, Zhang, Ziming Z, Upchurch, Sean S, Isern, Nancy N and Chen, Yuan Y.. (2004) Structure and DNA-binding sites of the SWI1 AT-rich interaction domain (ARID) suggest determinants for sequence-specific DNA recognition.. The Journal of biological chemistry, (16): [PMID:14722072]
16. Wang, Xiaomei X and 7 more authors.. (2004) Two related ARID family proteins are alternative subunits of human SWI/SNF complexes.. The Biochemical journal, (15): [PMID:15170388]
17. Gregory, S G SG and 178 more authors.. (2006) The DNA sequence and biological annotation of human chromosome 1.. Nature, (18): [PMID:16710414]
18. Beausoleil, Sean A SA, Villén, Judit J, Gerber, Scott A SA, Rush, John J and Gygi, Steven P SP.. (2006) A probability-based approach for high-throughput protein phosphorylation analysis and site localization.. Nature biotechnology, [PMID:16964243]
19. Matsuoka, Shuhei S and 12 more authors.. (2007) ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage.. Science (New York, N.Y.), (25): [PMID:17525332]
20. Cantin, Greg T GT and 6 more authors.. (2008) Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis.. Journal of proteome research, [PMID:18220336]
21. Dephoure, Noah N and 6 more authors.. (2008) A quantitative atlas of mitotic phosphorylation.. Proceedings of the National Academy of Sciences of the United States of America, (5): [PMID:18669648]
22. Lange, Martin M and 16 more authors.. (2008) Regulation of muscle development by DPF3, a novel histone acetylation and methylation reader of the BAF chromatin remodeling complex.. Genes & development, (1): [PMID:18765789]
23. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332]
24. Varela, Ignacio I and 43 more authors.. (2011) Exome sequencing identifies frequent mutation of the SWI/SNF complex gene PBRM1 in renal carcinoma.. Nature, (27): [PMID:21248752]
25. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692]
26. Jones, Siân S and 20 more authors.. (2012) Somatic mutations in the chromatin remodeling gene ARID1A occur in several tumor types.. Human mutation, [PMID:22009941]
27. Tsurusaki, Yoshinori Y and 30 more authors.. (2012) Mutations affecting components of the SWI/SNF complex cause Coffin-Siris syndrome.. Nature genetics, (18): [PMID:22426308]
28. Van Damme, Petra P and 15 more authors.. (2012) N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.. Proceedings of the National Academy of Sciences of the United States of America, (31): [PMID:22814378]
29. Euskirchen, Ghia G, Auerbach, Raymond K RK and Snyder, Michael M.. (2012) SWI/SNF chromatin-remodeling factors: multiscale analyses and diverse functions.. The Journal of biological chemistry, (7): [PMID:22952240]
30. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163]
31. Wieczorek, Dagmar D and 55 more authors.. (2013) A comprehensive molecular study on Coffin-Siris and Nicolaides-Baraitser syndromes identifies a broad molecular and clinical spectrum converging on altered chromatin remodeling.. Human molecular genetics, (20): [PMID:23906836]
32. Guo, Ailan and 15 more authors.. (2014) Immunoaffinity enrichment and mass spectrometry analysis of protein methylation.. Molecular & cellular proteomics : MCP, [PMID:24129315]
33. Bian, Yangyang Y and 9 more authors.. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.. Journal of proteomics, (16): [PMID:24275569]
34. Bateman, Nicholas W NW and 8 more authors.. (2016) Identification and functional characterization of a novel bipartite nuclear localization sequence in ARID1A.. Biochemical and biophysical research communications, (1): [PMID:26614907]
35. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460]

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SKU	Size	Availability	Price
Ab089579-10μl	10μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$69.90
Ab089579-50μl	50μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$199.90
Ab089579-100μl	100μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$309.90
Ab089579-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$2,399.90

ARID1A Antibody - Primary antibody, specific to ARID1A, Rabbit IgG