Recombinant Arp3 Antibody - Primary antibody, specific to ACTR3, Rabbit IgG

    Application:
  • IF/ICC
  • IHC
  • WB
Features and benefits
  • Host species: Rabbit
  • Species reactivity(Reacts with): Human,Mouse,Rat
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
Item Number
Ab089664
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab089664-10μl
10μl
In stock
$66.90
Ab089664-50μl
50μl
In stock
$199.90
Ab089664-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$289.90
Ab089664-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,279.90

Recombinant; Rabbit anti Human Arp3 Antibody; WB, IHC, ICC, IF; Unconjugated

Basic Description

Product NameRecombinant Arp3 Antibody - Primary antibody, specific to ACTR3, Rabbit IgG
SynonymsActin like protein 3 antibody | Actin related protein 3 antibody | ACTR3 antibody | ARP3 actin related protein 3 homolog (yeast) antibody | ACTR3 | ARP3
Specifications & PurityExactAb™, Validated, Recombinant, 0.3 mg/mL
Host speciesRabbit
SpecificityACTR3
ImmunogenA synthetic peptide derived from human Arp3 (AA 394-407).
Positive ControlWB: A549, A431, Jurkat, HeLa, C6 and NIH/3T3 cell lysates. IHC: Mouse colon tissue. ICC: HeLa cells.
ConjugationUnconjugated
GradeExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human Arp3 Antibody, Recombinant, could be used for WB, IHC, IF, ICC and so on.
Application
· WB: 1/500-1/1000
· IHC: 1/50-1/100
· IF/ICC: 1/50-1/200
Protein Relevance
The Arp2/3 complex involved in Golgi polerization in NIH3T3 cells. In a different model it has been shown that the Arp2/3 complex is necessary for neutrophil chemotaxis and phagocytosis.

Product Properties

IsotypeRabbit IgG
SDS-PAGE150 kDa
Purification MethodAntigen affinity purification
FormLiquid
Concentration0.3 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant Arp3 Antibody (Ab089664) - Western Blot
All lanes: Recombinant Arp3 Antibody (Ab089664) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 47 kDa
Observed band size: 45 kDa
Exposure time: 28.2 s

Recombinant Arp3 Antibody (Ab089664) - IF/ICC
Immunocytochemistry analysis of Arp3 (green) in HeLa cells using Recombinant Arp3 Antibody (Ab089664), and cells were counterstained with DAPI (blue).

Recombinant Arp3 Antibody (Ab089664) - IHC
Immunohistochemistry analysis of paraffin-embedded mouse colon using Recombinant Arp3 Antibody (Ab089664). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Associated Targets(Human)

ACTR3 Tchem Actin-related protein 3 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Mechanisms of Action

Mechanism of ActionAction Typetarget IDTarget NameTarget TypeTarget OrganismBinding Site NameReferences

Application

ApplicationDilution info
WB1/500-1/1000

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1/500-1/1000

IHC1/50 - 1/100

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1/50 - 1/100

IF/ICC1/50 - 1/200

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1/50 - 1/200

Certificates

Certificate of Analysis(COA)

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Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0202724Certificate of AnalysisFeb 05, 2024 Ab089664
ZJ24F0202723Certificate of AnalysisFeb 05, 2024 Ab089664

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