Recombinant Arp3 Antibody - Primary antibody, specific to ACTR3, Rabbit IgG
Synonyms
Actin like protein 3 antibody | Actin related protein 3 antibody | ACTR3 antibody | ARP3 actin related protein 3 homolog (yeast) antibody | ACTR3 | ARP3
Specifications & Purity
ExactAb™, Validated, Recombinant, 0.3 mg/mL
Host species
Rabbit
Specificity
ACTR3
Immunogen
A synthetic peptide derived from human Arp3 (AA 394-407).
Positive Control
WB: A549, A431, Jurkat, HeLa, C6 and NIH/3T3 cell lysates. IHC: Mouse colon tissue. ICC: HeLa cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human Arp3 Antibody, Recombinant, could be used for WB, IHC, IF, ICC and so on. Application · WB: 1/500-1/1000 · IHC: 1/50-1/100 · IF/ICC: 1/50-1/200 Protein Relevance The Arp2/3 complex involved in Golgi polerization in NIH3T3 cells. In a different model it has been shown that the Arp2/3 complex is necessary for neutrophil chemotaxis and phagocytosis.
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purification
Form
Liquid
Concentration
0.3 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant Arp3 Antibody (Ab089664) - Western Blot All lanes: Recombinant Arp3 Antibody (Ab089664) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 47 kDa Observed band size: 45 kDa Exposure time: 28.2 s
Recombinant Arp3 Antibody (Ab089664) - IF/ICC Immunocytochemistry analysis of Arp3 (green) in HeLa cells using Recombinant Arp3 Antibody (Ab089664), and cells were counterstained with DAPI (blue).
Recombinant Arp3 Antibody (Ab089664) - IHC Immunohistochemistry analysis of paraffin-embedded mouse colon using Recombinant Arp3 Antibody (Ab089664). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.