WB: MCF7, HEK293, A549, HeLa and K562 cell lysates.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human ATF3 Antibody, Recombinant, could be used for WB and so on. Application: WB: 1/500-1/1000 Protein Function: This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters.
Product Properties
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150kDa
Purification Method
Immunogen affinity purified
Form
Liquid
Concentration
0.5 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
1.Hai, T W TW, Liu, F F, Coukos, W J WJ and Green, M R MR.. (1989) Transcription factor ATF cDNA clones: an extensive family of leucine zipper proteins able to selectively form DNA-binding heterodimers.. Genes & development, [PMID:2516827]
2.Chen, B P BP, Liang, G G, Whelan, J J and Hai, T T.. (1994) ATF3 and ATF3 delta Zip. Transcriptional repression versus activation by alternatively spliced isoforms.. The Journal of biological chemistry, (3):[PMID:7515060]
3.Hashimoto, Yoshinori Y and 8 more authors.. (2002) An alternatively spliced isoform of transcriptional repressor ATF3 and its induction by stress stimuli.. Nucleic acids research, (1):[PMID:12034827]
4.Pan, YuanXiang Y, Chen, Hong H, Siu, Fai F and Kilberg, Michael S MS.. (2003) Amino acid deprivation and endoplasmic reticulum stress induce expression of multiple activating transcription factor-3 mRNA species that, when overexpressed in HepG2 cells, modulate transcription by the human asparagine synthetase promoter.. The Journal of biological chemistry, (3):[PMID:12881527]
5.Hillman, R Tyler RT, Green, Richard E RE and Brenner, Steven E SE.. (2004) An unappreciated role for RNA surveillance.. Genome biology, [PMID:14759258]
6.Gregory, S G SG and 178 more authors.. (2006) The DNA sequence and biological annotation of human chromosome 1.. Nature, (18):[PMID:16710414]
7.Fiebich, Bernd L BL and 6 more authors.. (2012) Pseudoephedrine inhibits T-cell activation by targeting NF-κB, NFAT and AP-1 signaling pathways.. Immunopharmacology and immunotoxicology, [PMID:21631396]
8.Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4):[PMID:23186163]
9.Cui, Hongmei H and 8 more authors.. (2015) The stress-responsive gene ATF3 regulates the histone acetyltransferase Tip60.. Nature communications, (13):[PMID:25865756]
10.Eising, Else E and 15 more authors.. (2019) A set of regulatory genes co-expressed in embryonic human brain is implicated in disrupted speech development.. Molecular psychiatry, [PMID:29463886]
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