| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab089976-10μl | 10μl | In stock | $66.90 | |
| Ab089976-50μl | 50μl | In stock | $199.90 | |
| Ab089976-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| Ab089976-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,699.90 |
Recombinant; Rabbit anti Human ATG12 Antibody; WB; Unconjugated
| Product Name | Recombinant ATG12 Antibody - Primary antibody, specific to ATG12, Rabbit IgG |
|---|---|
| Synonyms | Apg12 (autophagy 12, S. cerevisiae)-like | APG12 autophagy 12-like (S. cerevisiae) | APG12 | APG12HAPG12 | APG12-like | ATG12 autophagy related 12 homolog (S. cerevisiae) | ATG12 | Autophagy-related protein 12 | FBR93 | HAPG12 | ubiquitin-like protein ATG |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 1.6 mg/mL |
| Host species | Rabbit |
| Specificity | ATG12 |
| Immunogen | A synthetic peptide derived from human Apg12 (AA 123-140) |
| Positive Control | WB: K562 and Hep G2 cell lysates. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human ATG12 Antibody, Recombinant, could be used for WB and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified |
| Form | Liquid |
| Concentration | 1.6 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant ATG12 Antibody (Ab089976) - Western Blot
All lanes: Recombinant ATG12 Antibody (Ab089976) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 15 kDa
Observed band size: 21, 52 kDa
Exposure time: 50.0 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000"> 1/500-1/1000 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0303191 | Certificate of Analysis | Mar 13, 2024 | Ab089976 |
| ZJ24F0303190 | Certificate of Analysis | Mar 13, 2024 | Ab089976 |
| 1. Mizushima, N N, Sugita, H H, Yoshimori, T T and Ohsumi, Y Y.. (1998) A new protein conjugation system in human. The counterpart of the yeast Apg12p conjugation system essential for autophagy.. The Journal of biological chemistry, (18): [PMID:9852036] |
| 2. Tanida, I I, Tanida-Miyake, E E, Ueno, T T and Kominami, E E.. (2001) The human homolog of Saccharomyces cerevisiae Apg7p is a Protein-activating enzyme for multiple substrates including human Apg12p, GATE-16, GABARAP, and MAP-LC3.. The Journal of biological chemistry, (19): [PMID:11096062] |
| 3. Tanida, Isei I, Tanida-Miyake, Emiko E, Komatsu, Masaaki M, Ueno, Takashi T and Kominami, Eiki E.. (2002) Human Apg3p/Aut1p homologue is an authentic E2 enzyme for multiple substrates, GATE-16, GABARAP, and MAP-LC3, and facilitates the conjugation of hApg12p to hApg5p.. The Journal of biological chemistry, (19): [PMID:11825910] |
| 4. Tanida, Isei I, Nishitani, Tomohito T, Nemoto, Takahiro T, Ueno, Takashi T and Kominami, Eiki E.. (2002) Mammalian Apg12p, but not the Apg12p.Apg5p conjugate, facilitates LC3 processing.. Biochemical and biophysical research communications, (6): [PMID:12207896] |
| 5. Schmutz, Jeremy J and 75 more authors.. (2004) The DNA sequence and comparative analysis of human chromosome 5.. Nature, (16): [PMID:15372022] |
| 6. Shao, Yufang Y, Gao, Zhonghua Z, Feldman, Taya T and Jiang, Xuejun X.. () Stimulation of ATG12-ATG5 conjugation by ribonucleic acid.. Autophagy, [PMID:16963840] |
| 7. Prigione, Alessandro A and Cortopassi, Gino G.. () Mitochondrial DNA deletions and chloramphenicol treatment stimulate the autophagic transcript ATG12.. Autophagy, [PMID:17457038] |
| 8. Jounai, Nao N and 10 more authors.. (2007) The Atg5 Atg12 conjugate associates with innate antiviral immune responses.. Proceedings of the National Academy of Sciences of the United States of America, (28): [PMID:17709747] |
| 9. Kim, Peter Kijun PK, Hailey, Dale Warren DW, Mullen, Robert Thomas RT and Lippincott-Schwartz, Jennifer J.. (2008) Ubiquitin signals autophagic degradation of cytosolic proteins and peroxisomes.. Proceedings of the National Academy of Sciences of the United States of America, (30): [PMID:19074260] |
| 10. Lee, In Hye IH and Finkel, Toren T.. (2009) Regulation of autophagy by the p300 acetyltransferase.. The Journal of biological chemistry, (6): [PMID:19124466] |
| 11. Chen, Dandan D and 5 more authors.. (2012) A mammalian autophagosome maturation mechanism mediated by TECPR1 and the Atg12-Atg5 conjugate.. Molecular cell, (9): [PMID:22342342] |
| 12. Otomo, Chinatsu C, Metlagel, Zoltan Z, Takaesu, Giichi G and Otomo, Takanori T.. (2013) Structure of the human ATG12~ATG5 conjugate required for LC3 lipidation in autophagy.. Nature structural & molecular biology, [PMID:23202584] |
| 13. Kim, Myungjin M and 21 more authors.. (2016) Mutation in ATG5 reduces autophagy and leads to ataxia with developmental delay.. eLife, (26): [PMID:26812546] |
| 14. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460] |
