| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab090068-10μl | 10μl | In stock | $66.90 | |
| Ab090068-50μl | 50μl | In stock | $189.90 | |
| Ab090068-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| Ab090068-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,699.90 |
Recombinant; Rabbit anti Human ATP citrate lyase Antibody; WB, ICC, IF; Unconjugated
| Product Name | Recombinant ATP citrate lyase Antibody - Primary antibody, specific to ACLY, Rabbit IgG |
|---|---|
| Synonyms | ACL antibody | Acly antibody | ACLY_HUMAN antibody | ATP citrate (pro-S) lyase antibody | ATP citrate lyase antibody | ATP citrate synthase antibody | ATP-citrate (pro-S-)-lyase antibody | ATP-citrate synthase antibody | ATPcitrate synthase antibody | ATP |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.3 mg/mL |
| Host species | Rabbit |
| Specificity | ACLY |
| Immunogen | A synthetic peptide derived from human ATP citrate lyase (AA 1083-1011) |
| Positive Control | WB: K562, HeLa, NIH/3T3 and C6 cell lysates. ICC/IF: A549 cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description |
Rabbit anti Human ATP citrate lyase Antibody, Recombinant, could be used for WB, ICC, IF and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified |
| Form | Liquid |
| Concentration | 0.3 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant ATP citrate lyase Antibody (Ab090068) - Western Blot
All lanes: Recombinant ATP citrate lyase Antibody (Ab090068) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 121 kDa
Observed band size: 117 kDa
Exposure time: 22.8 s
Recombinant ATP citrate lyase Antibody (Ab090068) - IF/ICC
Immunocytochemistry analysis of ATP Citrate lyase (green) in A549 using Recombinant ATP citrate lyase Antibody (Ab090068), and cells were counterstained with DAPI (blue).
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000"> 1/500-1/1000 |
| IF/ICC |
1/50-1/200
">
1/50-1/200
|
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0303193 | Certificate of Analysis | Mar 13, 2024 | Ab090068 |
| ZJ24F0303192 | Certificate of Analysis | Mar 13, 2024 | Ab090068 |
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| 8. Jiao WH, Li J, Wang D, Zhang MM, Liu LY, Sun F, Li JY, Capon RJ, Lin HW.. (2019) Cinerols, Nitrogenous Meroterpenoids from the Marine Sponge Dysidea cinerea.. J Nat Prod, 82 (9): (2586-2593). [PMID:31532203] [10.1021/acs.jnatprod.9b00471] |
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| 11. Rush, John J and 9 more authors.. (2005) Immunoaffinity profiling of tyrosine phosphorylation in cancer cells.. Nature biotechnology, [PMID:15592455] |
| 12. Giannakopoulos, Nadia V NV and 9 more authors.. (2005) Proteomic identification of proteins conjugated to ISG15 in mouse and human cells.. Biochemical and biophysical research communications, (21): [PMID:16139798] |
| 13. Zody, Michael C MC and 73 more authors.. (2006) DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage.. Nature, (20): [PMID:16625196] |
| 14. Yu, Li-Rong LR and 5 more authors.. (2007) Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra.. Journal of proteome research, [PMID:17924679] |
| 15. Zahedi, René P RP and 8 more authors.. (2008) Phosphoproteome of resting human platelets.. Journal of proteome research, [PMID:18088087] |
| 16. Dephoure, Noah N and 6 more authors.. (2008) A quantitative atlas of mitotic phosphorylation.. Proceedings of the National Academy of Sciences of the United States of America, (5): [PMID:18669648] |
| 17. Daub, Henrik H and 9 more authors.. (2008) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle.. Molecular cell, (8): [PMID:18691976] |
| 18. Ma, Zhengping Z, Chu, Ching-Hsuen CH and Cheng, Dong D.. (2009) A novel direct homogeneous assay for ATP citrate lyase.. Journal of lipid research, [PMID:19286649] |
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| 20. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332] |
| 21. Sun, Tianjun T, Hayakawa, Koto K, Bateman, Katherine S KS and Fraser, Marie E ME.. (2010) Identification of the citrate-binding site of human ATP-citrate lyase using X-ray crystallography.. The Journal of biological chemistry, (27): [PMID:20558738] |
| 22. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692] |
| 23. Sun, Tianjun T, Hayakawa, Koto K and Fraser, Marie E ME.. (2011) ADP-Mg2+ bound to the ATP-grasp domain of ATP-citrate lyase.. Acta crystallographica. Section F, Structural biology and crystallization communications, (1): [PMID:22102020] |
| 24. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
| 25. Lin, Ruiting R and 7 more authors.. (2013) Acetylation stabilizes ATP-citrate lyase to promote lipid biosynthesis and tumor growth.. Molecular cell, (22): [PMID:23932781] |
| 26. Bian, Yangyang Y and 9 more authors.. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.. Journal of proteomics, (16): [PMID:24275569] |
| 27. Zhang, Cen C and 11 more authors.. (2016) Cullin3-KLHL25 ubiquitin ligase targets ACLY for degradation to inhibit lipid synthesis and tumor progression.. Genes & development, (1): [PMID:27664236] |
| 28. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460] |
