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Recombinant ATP citrate lyase Antibody - Primary antibody, specific to ACLY, Rabbit IgG

  • ExactAb™
  • Recombinant
  • Validated
  • 0.3 mg/mL
Features and benefits
  • Short Overview:

    Recombinant; Rabbit anti Human ATP citrate lyase Antibody; WB, ICC, IF; Unconjugated

  • Species reactivity(Reacts with): Human,Mouse,Rat
  • Isotype: Rabbit IgG
    Application:
  • IF/ICC
  • WB
Item Number
Ab090068
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab090068-10μl
10μl
In stock
$66.90
Ab090068-50μl
50μl
In stock
$189.90
Ab090068-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$299.90
Ab090068-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,699.90

Recombinant; Rabbit anti Human ATP citrate lyase Antibody; WB, ICC, IF; Unconjugated

Basic Description

Product NameRecombinant ATP citrate lyase Antibody - Primary antibody, specific to ACLY, Rabbit IgG
SynonymsACL antibody; Acly antibody; ACLY_HUMAN antibody; ATP citrate (pro-S) lyase antibody; ATP citrate lyase antibody; ATP citrate synthase antibody; ATP-citrate (pro-S-)-lyase antibody; ATP-citrate synthase antibody; ATPcitrate synthase antibody; ATPCL antibo
Specifications & PurityExactAb™, Validated, Recombinant, 0.3 mg/mL
Host speciesRabbit
SpecificityACLY
ImmunogenA synthetic peptide derived from human ATP citrate lyase (AA 1083-1011)
Positive ControlWB: K562, HeLa, NIH/3T3 and C6 cell lysates. ICC/IF: A549 cells.
ConjugationUnconjugated
GradeExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human ATP citrate lyase Antibody, Recombinant, could be used for WB, ICC, IF and so on.
Application:
WB: 1/500-1/1000
ICC/IF: 1/50-1/200
Protein Function
ATP-citrate synthase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine.

Product Properties

IsotypeRabbit IgG
SDS-PAGE150 kDa
Purification MethodAntigen affinity purified
FormLiquid
Concentration0.3 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant ATP citrate lyase Antibody (Ab090068) - Western Blot
All lanes: Recombinant ATP citrate lyase Antibody (Ab090068) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 121 kDa
Observed band size: 117 kDa
Exposure time: 22.8 s

Recombinant ATP citrate lyase Antibody (Ab090068) - IF/ICC
Immunocytochemistry analysis of ATP Citrate lyase (green) in A549 using Recombinant ATP citrate lyase Antibody (Ab090068), and cells were counterstained with DAPI (blue).

Associated Targets

ACLY Tclin ATP-citrate synthase 0 Activities

Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
WB

1/500-1/1000

ICC/IF

1/50-1/200

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section

2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0303193Certificate of AnalysisMar 13, 2024 Ab090068
ZJ24F0303192Certificate of AnalysisMar 13, 2024 Ab090068

Related Documents

References

1. Elshourbagy NA, Near JC, Kmetz PJ, Wells TN, Groot PH, Saxty BA, Hughes SA, Franklin M, Gloger IS.  (1992)  Cloning and expression of a human ATP-citrate lyase cDNA..  Eur J Biochem,  204  (2): (491-9).  [PMID:1371749]
2. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.  (2009)  Lysine acetylation targets protein complexes and co-regulates major cellular functions..  Science,  325  (5942): (834-40).  [PMID:19608861]
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7. Jernigan FE, Hanai JI, Sukhatme VP, Sun L..  (2017)  Discovery of furan carboxylate derivatives as novel inhibitors of ATP-citrate lyase via virtual high-throughput screening..  Bioorg Med Chem Lett,  27  (4): (929-935).  [PMID:28129980]
8. Jiao WH, Li J, Wang D, Zhang MM, Liu LY, Sun F, Li JY, Capon RJ, Lin HW..  (2019)  Cinerols, Nitrogenous Meroterpenoids from the Marine Sponge Dysidea cinerea..  J Nat Prod,  82  (9): (2586-2593).  [PMID:31532203]
9. Lord, K A KA and 10 more authors..  (1997)  Variant cDNA sequences of human ATP:citrate lyase: cloning, expression, and purification from baculovirus-infected insect cells..  Protein expression and purification,      [PMID:9116495]
10. Potapova, I A IA, El-Maghrabi, M R MR, Doronin, S V SV and Benjamin, W B WB..  (2000)  Phosphorylation of recombinant human ATP:citrate lyase by cAMP-dependent protein kinase abolishes homotropic allosteric regulation of the enzyme by citrate and increases the enzyme activity. Allosteric activation of ATP:citrate lyase by phosphorylated sugars..  Biochemistry,    (8):   [PMID:10653665]
11. Rush, John J and 9 more authors..  (2005)  Immunoaffinity profiling of tyrosine phosphorylation in cancer cells..  Nature biotechnology,      [PMID:15592455]
12. Giannakopoulos, Nadia V NV and 9 more authors..  (2005)  Proteomic identification of proteins conjugated to ISG15 in mouse and human cells..  Biochemical and biophysical research communications,    (21):   [PMID:16139798]
13. Zody, Michael C MC and 73 more authors..  (2006)  DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage..  Nature,    (20):   [PMID:16625196]
14. Yu, Li-Rong LR and 5 more authors..  (2007)  Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra..  Journal of proteome research,      [PMID:17924679]
15. Zahedi, René P RP and 8 more authors..  (2008)  Phosphoproteome of resting human platelets..  Journal of proteome research,      [PMID:18088087]
16. Dephoure, Noah N and 6 more authors..  (2008)  A quantitative atlas of mitotic phosphorylation..  Proceedings of the National Academy of Sciences of the United States of America,    (5):   [PMID:18669648]
17. Daub, Henrik H and 9 more authors..  (2008)  Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle..  Molecular cell,    (8):   [PMID:18691976]
18. Ma, Zhengping Z, Chu, Ching-Hsuen CH and Cheng, Dong D..  (2009)  A novel direct homogeneous assay for ATP citrate lyase..  Journal of lipid research,      [PMID:19286649]
19. Oppermann, Felix S FS and 7 more authors..  (2009)  Large-scale proteomics analysis of the human kinome..  Molecular & cellular proteomics : MCP,      [PMID:19369195]
20. Mayya, Viveka V and 7 more authors..  (2009)  Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions..  Science signaling,    (18):   [PMID:19690332]
21. Sun, Tianjun T, Hayakawa, Koto K, Bateman, Katherine S KS and Fraser, Marie E ME..  (2010)  Identification of the citrate-binding site of human ATP-citrate lyase using X-ray crystallography..  The Journal of biological chemistry,    (27):   [PMID:20558738]
22. Rigbolt, Kristoffer T G KT and 9 more authors..  (2011)  System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation..  Science signaling,    (15):   [PMID:21406692]
23. Sun, Tianjun T, Hayakawa, Koto K and Fraser, Marie E ME..  (2011)  ADP-Mg2+ bound to the ATP-grasp domain of ATP-citrate lyase..  Acta crystallographica. Section F, Structural biology and crystallization communications,    (1):   [PMID:22102020]
24. Zhou, Houjiang H and 6 more authors..  (2013)  Toward a comprehensive characterization of a human cancer cell phosphoproteome..  Journal of proteome research,    (4):   [PMID:23186163]
25. Lin, Ruiting R and 7 more authors..  (2013)  Acetylation stabilizes ATP-citrate lyase to promote lipid biosynthesis and tumor growth..  Molecular cell,    (22):   [PMID:23932781]
26. Bian, Yangyang Y and 9 more authors..  (2014)  An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome..  Journal of proteomics,    (16):   [PMID:24275569]
27. Zhang, Cen C and 11 more authors..  (2016)  Cullin3-KLHL25 ubiquitin ligase targets ACLY for degradation to inhibit lipid synthesis and tumor progression..  Genes & development,    (1):   [PMID:27664236]

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