| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab128631-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $49.90 | |
| Ab128631-50μl | 50μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $147.90 | |
| Ab128631-100μl | 100μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $237.90 | |
| Ab128631-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $1,707.90 |
Recombinant (RR685); Rabbit anti Human ATP1A1 Antibody; WB, IHC, Flow, IP; Unconjugated
| Product Name | Recombinant ATP1A1 Antibody - Primary antibody, specific to ATP1A1, Rabbit IgG |
|---|---|
| Synonyms | ATPase Na+/K+ transporting alpha antibody | adenosinetriphosphatase antibody | AT1A1_HUMAN antibody | ATP1A1 antibody | ATP1A4 antibody | ATP1AL2 antibody | ATP1B antibody | ATP1B1 antibody | ATPase Na+/K+ transporting alpha 1 polypeptide antibody | ATPas |
| Specifications & Purity | ExactAb™, Validated, Recombinant, High performance, 0.5 mg/mL |
| Host species | Rabbit |
| Specificity | ATP1A1 |
| Immunogen | A synthetic peptide corresponding to residues on the N-terminus of human NaK ATPase was used as an immunogen. |
| Positive Control | WB: C6, HT29, A431, Hep G2, and Raw264.7 cell lysates. IHC: Endometrium tissue. Flow: HeLa cells. IP: TT whole cell lysate. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, High Performance, Recombinant, Validated |
| Product Description | Rabbit anti Human ATP1A1 Antibody, Recombinant antibody (RR685), could be used for WB, IHC, Flow, IP and so on. Application: WB: 1/2000 - 1/5000 IHC: 1/10000 - 1/20000 Flow: 1/50 - 1/200 IP: 1/50 Protein Function This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. Post-translational Phosphorylation on Tyr-10 modulates pumping activity. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant ATP1A1 Antibody (Ab128631) - Western Blot
All lanes: Recombinant ATP1A1 Antibody (Ab128631) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 100 kDa
Observed band size: 97 kDa
Exposure time: 32.2 s
Recombinant ATP1A1 Antibody (Ab128631) - Western Blot
All lanes: Recombinant ATP1A1 Antibody (Ab128631) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 100 kDa
Observed band size: 97 kDa
Exposure time: 10.0 s
Recombinant ATP1A1 Antibody (Ab128631) - IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of endometrium tissue labelling NaK ATPase with Recombinant ATP1A1 Antibody (Ab128631) at 1/10000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.
Recombinant ATP1A1 Antibody (Ab128631) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Recombinant ATP1A1 Antibody (Ab128631) (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in the Recombinant ATP1A1 Antibody (Ab128631) (1/200 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody used was a Goat Anti-Rabbit Alexa Fluor® 488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabeled sample (Black) was used as a control.
Recombinant ATP1A1 Antibody (Ab128631) - IP
NaK ATPase was immunoprecipitated from 0.4 mg of TT whole cell lysate with Recombinant ATP1A1 Antibody (Ab128631) at 1/50 dilution
2nd Ab: GAR HRP for IP 1/500
Lane 1: Recombinant ATP1A1 Antibody (Ab128631) IP in TT whole cell lysate
Lane 2: PBS instead of Recombinant ATP1A1 Antibody (Ab128631) in TT whole cell lysate
Lane 3: TT whole cell lysate, 10 μg (input)
Exposure: 120 s.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
| Application | Dilution info |
|---|---|
| IP |
1/50
|
| WB |
1/2000 - 1/5000
|
| Flow Cytometry | 1/50 - 1/200 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F0901094 | Certificate of Analysis | Sep 18, 2023 | Ab128631 |
| ZJ23F0901095 | Certificate of Analysis | Sep 18, 2023 | Ab128631 |
