| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab090333-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $66.90 | |
| Ab090333-50μl | 50μl | In stock | $199.90 | |
| Ab090333-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| Ab090333-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,699.90 |
Recombinant; Rabbit anti Human Axin 2 Antibody; WB; Unconjugated
| Product Name | Recombinant Axin 2 Antibody - Primary antibody, specific to AXIN2, Rabbit IgG |
|---|---|
| Synonyms | AXIL | axin 2 | Axin2 | Axin-2 | Axin-like protein | Axis inhibition protein 2 | Conductin | DKFZp781B0869 | MGC10366 | MGC126582 | Axil antibody | Axin like protein antibody | Axin-2 antibody | Axin-like protein antibody | Axin2 antibody | AXIN2_HUMAN an |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 2.0 mg/mL |
| Host species | Rabbit |
| Specificity | AXIN2 |
| Immunogen | A synthetic peptide derived from human Axin 2 (AA 67-81) |
| Positive Control | WB: Jurkat, C6, NIH/3T3 and HEK293 cell lysates. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description |
Rabbit anti Human Axin 2 Antibody, Recombinant, could be used for WB and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Immunogen affinity purified |
| Form | Liquid |
| Concentration | 2.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant Axin 2 Antibody (Ab090333) - Western Blot
All lanes: Recombinant Axin 2 Antibody (Ab090333) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 94 kDa
Observed band size: 84 kDa
Exposure time: 150.2 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000"> 1/500-1/1000 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F1202201 | Certificate of Analysis | Dec 26, 2023 | Ab090333 |
| ZJ23F1202202 | Certificate of Analysis | Dec 26, 2023 | Ab090333 |
| 1. Chen B et al.. (2009) Small molecule-mediated disruption of Wnt-dependent signaling in tissue regeneration and cancer.. Nat Chem Biol, 5 (2): (100-7). [PMID:19125156] |
| 2. Shultz MD, Kirby CA, Stams T, Chin DN, Blank J, Charlat O, Cheng H, Cheung A, Cong F, Feng Y, Fortin PD, Hood T, Tyagi V, Xu M, Zhang B, Shao W.. (2012) [1,2,4]triazol-3-ylsulfanylmethyl)-3-phenyl-[1,2,4]oxadiazoles: antagonists of the Wnt pathway that inhibit tankyrases 1 and 2 via novel adenosine pocket binding.. J Med Chem, 55 (3): (1127-1136). [PMID:22260203] [10.1021/jm2011222] |
| 3. Mai, M M, Qian, C C, Yokomizo, A A, Smith, D I DI and Liu, W W.. (1999) Cloning of the human homolog of conductin (AXIN2), a gene mapping to chromosome 17q23-q24.. Genomics, (1): [PMID:10049590] |
| 4. Liu, W W and 14 more authors.. (2000) Mutations in AXIN2 cause colorectal cancer with defective mismatch repair by activating beta-catenin/TCF signalling.. Nature genetics, [PMID:11017067] |
| 5. Lammi, Laura L and 7 more authors.. (2004) Mutations in AXIN2 cause familial tooth agenesis and predispose to colorectal cancer.. American journal of human genetics, [PMID:15042511] |
| 6. Liu, Wei W and 12 more authors.. (2006) Axin is a scaffold protein in TGF-beta signaling that promotes degradation of Smad7 by Arkadia.. The EMBO journal, (19): [PMID:16601693] |
| 7. Zody, Michael C MC and 73 more authors.. (2006) DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage.. Nature, (20): [PMID:16625196] |
| 8. Kanzaki, Hirotaka H and 9 more authors.. (2006) Single nucleotide polymorphism of the AXIN2 gene is preferentially associated with human lung cancer risk in a Japanese population.. International journal of molecular medicine, [PMID:16820935] |
| 9. Huang, Shih-Min A SM and 35 more authors.. (2009) Tankyrase inhibition stabilizes axin and antagonizes Wnt signalling.. Nature, (1): [PMID:19759537] |
| 10. Lui, Tony T H TT and 6 more authors.. (2011) The ubiquitin-specific protease USP34 regulates axin stability and Wnt/β-catenin signaling.. Molecular and cellular biology, [PMID:21383061] |
| 11. Zhang, Yue Y and 14 more authors.. (2011) RNF146 is a poly(ADP-ribose)-directed E3 ligase that regulates axin degradation and Wnt signalling.. Nature cell biology, [PMID:21478859] |
| 12. Ji, Lei L and 9 more authors.. (2017) The SIAH E3 ubiquitin ligases promote Wnt/β-catenin signaling through mediating Wnt-induced Axin degradation.. Genes & development, (1): [PMID:28546513] |
