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Ab090512-10μl

10μl

In stock

$69.90

Ab090512-50μl

50μl

In stock

$189.90

Ab090512-100μl

100μl

Available within 8-12 weeks(?)

$299.90

Ab090512-1ml

1ml

Available within 8-12 weeks(?)

$2,699.90

Basic Description

Product Name	Recombinant BAF57/SMARCE1 Antibody - Primary antibody, specific to SMARCE1, Rabbit IgG
Synonyms	BAF57 antibody \| BRG1 associated factor 57 antibody \| BRG1-associated factor 57 antibody \| Chromatin remodeling complex BRG1 associated factor 57 antibody \| FLJ35648 antibody \| SMARCE 1 antibody \| SMARCE1 antibody \| SMCE1_HUMAN antibody \| SWI/SNF related
Specifications & Purity	ExactAb™, Validated, Recombinant, 0.2 mg/mL
Host species	Rabbit
Specificity	SMARCE1
Immunogen	A synthetic peptide derived from human BAF57/SMARCE1 (AA 1-59)
Positive Control	WB: Raji, MCF7, HeLa, Jurkat, NIH/3T3 and C6 cell lysates.
Conjugation	Unconjugated
Grade	ExactAb™, Recombinant, Validated
Product Description	Rabbit anti Human SMARCE1 Antibody, Recombinant, could be used for WB and so on. Application: WB: 1/500-1/1000 Protein Function Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Required for the coactivation of estrogen responsive promoters by Swi/Snf complexes and the SRC/p160 family of histone acetyltransferases (HATs). Also specifically interacts with the CoREST corepressor resulting in repression of neuronal specific gene promoters in non-neuronal cells. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.

Product Properties

Isotype	Rabbit IgG
Light Chain Type	kappa
SDS-PAGE	150 kDa
Purification Method	Protein A purified
Form	Liquid
Concentration	0.2 mg/mL
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant BAF57/SMARCE1 Antibody (Ab090512) - Western Blot
All lanes: Recombinant BAF57/SMARCE1 Antibody (Ab090512) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 47 kDa
Observed band size: 52 kDa
Exposure time: 10.7 s

Associated Targets(Human)

SMARCE1 Tbio SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1 (0 Activities)

Discover Target: SMARCE1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Application

Application	Dilution info
WB	1/500-1/1000

Certificates

Certificate of Analysis(COA)

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2 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0404920	Certificate of Analysis	Apr 25, 2024	Ab090512
ZJ24F0404919	Certificate of Analysis	Apr 25, 2024	Ab090512

FAQs and Articles

FAQ

Protocol of Western Blotting For High Molecular Weights

Categories: Protocols

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Western blotting Biological buffer Immunoblotting Protein Electrophoresis High Molecular Weights large molecular proteins WB antibody

References

1. Hsiao PW, Fryer CJ, Trotter KW, Wang W, Archer TK. (2003) BAF60a mediates critical interactions between nuclear receptors and the BRG1 chromatin-remodeling complex for transactivation.. Mol Cell Biol, 23 (17): (6210-20). [PMID:12917342]
2. Kadoch C, Crabtree GR. (2015) Mammalian SWI/SNF chromatin remodeling complexes and cancer: Mechanistic insights gained from human genomics.. Sci Adv, 1 (5): (e1500447). [PMID:26601204]
3. Francis Impens,Lilliana Radoshevich,Pascale Cossart,David Ribet. (2014-08-13) Mapping of SUMO sites and analysis of SUMOylation changes induced by external stimuli.. Proceedings of the National Academy of Sciences of the United States of America, 111 ((34)): (12432-12437). [PMID:25114211]
4. Wang, W W and 5 more authors.. (1998) Architectural DNA binding by a high-mobility-group/kinesin-like subunit in mammalian SWI/SNF-related complexes.. Proceedings of the National Academy of Sciences of the United States of America, (20): [PMID:9435219]
5. Chi, Tian H TH and 6 more authors.. (2002) Reciprocal regulation of CD4/CD8 expression by SWI/SNF-like BAF complexes.. Nature, (11): [PMID:12110891]
6. Belandia, Borja B, Orford, Rob L RL, Hurst, Helen C HC and Parker, Malcolm G MG.. (2002) Targeting of SWI/SNF chromatin remodelling complexes to estrogen-responsive genes.. The EMBO journal, (1): [PMID:12145209]
7. Battaglioli, Elena E and 6 more authors.. (2002) REST repression of neuronal genes requires components of the hSWI.SNF complex.. The Journal of biological chemistry, (25): [PMID:12192000]
8. Martens, Joseph A JA and Winston, Fred F.. (2003) Recent advances in understanding chromatin remodeling by Swi/Snf complexes.. Current opinion in genetics & development, [PMID:12672490]
9. Huang, Chun-Yin CY and 7 more authors.. (2005) hZimp7, a novel PIAS-like protein, enhances androgen receptor-mediated transcription and interacts with SWI/SNF-like BAF complexes.. Molecular endocrinology (Baltimore, Md.), [PMID:16051670]
10. Zody, Michael C MC and 73 more authors.. (2006) DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage.. Nature, (20): [PMID:16625196]
11. Lange, Martin M and 16 more authors.. (2008) Regulation of muscle development by DPF3, a novel histone acetylation and methylation reader of the BAF chromatin remodeling complex.. Genes & development, (1): [PMID:18765789]
12. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332]
13. Keppler, Brian R BR and Archer, Trevor K TK.. (2010) Ubiquitin-dependent and ubiquitin-independent control of subunit stoichiometry in the SWI/SNF complex.. The Journal of biological chemistry, (12): [PMID:20829358]
14. Mi, Lixin and 8 more authors.. (2011) Identification of potential protein targets of isothiocyanates by proteomics.. Chemical research in toxicology, (17): [PMID:21838287]
15. Tsurusaki, Yoshinori Y and 30 more authors.. (2012) Mutations affecting components of the SWI/SNF complex cause Coffin-Siris syndrome.. Nature genetics, (18): [PMID:22426308]
16. Euskirchen, Ghia G, Auerbach, Raymond K RK and Snyder, Michael M.. (2012) SWI/SNF chromatin-remodeling factors: multiscale analyses and diverse functions.. The Journal of biological chemistry, (7): [PMID:22952240]
17. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163]
18. Smith, Miriam J MJ and 12 more authors.. (2013) Loss-of-function mutations in SMARCE1 cause an inherited disorder of multiple spinal meningiomas.. Nature genetics, [PMID:23377182]
19. Wieczorek, Dagmar D and 55 more authors.. (2013) A comprehensive molecular study on Coffin-Siris and Nicolaides-Baraitser syndromes identifies a broad molecular and clinical spectrum converging on altered chromatin remodeling.. Human molecular genetics, (20): [PMID:23906836]
20. Guo, Ailan and 15 more authors.. (2014) Immunoaffinity enrichment and mass spectrometry analysis of protein methylation.. Molecular & cellular proteomics : MCP, [PMID:24129315]
21. Raffalli-Ebezant, Helen H and 6 more authors.. (2015) Pediatric intracranial clear cell meningioma associated with a germline mutation of SMARCE1: a novel case.. Child's nervous system : ChNS : official journal of the International Society for Pediatric Neurosurgery, [PMID:25249420]
22. Xiao, Zhenyu Z and 5 more authors.. (2015) System-wide Analysis of SUMOylation Dynamics in Response to Replication Stress Reveals Novel Small Ubiquitin-like Modified Target Proteins and Acceptor Lysines Relevant for Genome Stability.. Molecular & cellular proteomics : MCP, [PMID:25755297]
23. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460]

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Recombinant BAF57/SMARCE1 Antibody - Primary antibody, specific to SMARCE1, Rabbit IgG