Recombinant (RR647); Rabbit anti Human Bax Antibody; WB, IHC, Flow, IP; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab090652-10μl | 10μl | In stock | $59.90 | |
| Ab090652-50μl | 50μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $177.90 | |
| Ab090652-100μl | 100μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $297.90 | |
| Ab090652-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,217.90 |
Recombinant (RR647); Rabbit anti Human Bax Antibody; WB, IHC, Flow, IP; Unconjugated
| Product Name | Recombinant Bax Antibody - Primary antibody, specific to Bax, Rabbit IgG |
|---|---|
| Synonyms | Apoptosis regulator BAX antibody; BAX antibody; Bax-protein antibody; BAX_HUMAN antibody; BAXA antibody; Baxdelta2G9 antibody; Baxdelta2G9omega antibody; Baxdelta2omega antibody; Bcl-2-like protein 4 antibody; BCL2 associated X protein antibody; BCL2 asso |
| Specifications & Purity | ExactAb™, Validated, Recombinant, High performance, 0.25mg/mL |
| Host species | Rabbit |
| Specificity | Bax |
| Immunogen | A synthetic peptide of human BAX (AA 1-100). |
| Positive Control | WB: A549, HT-29, HeLa, LNCAP, Hep G2 and HKE293 cell lysates. IHC: Human Ovarian cancer tissue. Flow: HeLa cells. IP: HT-29 whole cell lysate. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, High Performance, Recombinant, Validated |
| Product Description | Rabbit anti Human Bax Antibody, Recombinant (RR647), could be used for WB, IHC, Flow, IP and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 0.25mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant Bax Antibody (Ab090652) - Western Blot
All lanes: Recombinant Bax Antibody (Ab090652) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 21 kDa
Observed band size: 20 kDa
Exposure time: 6.2 s
Recombinant Bax Antibody (Ab090652) - IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian cancer tissue labelling Bax with Recombinant Bax Antibody (Ab090652) at 1/1600. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.
Recombinant Bax Antibody (Ab090652) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Recombinant Bax Antibody (Ab090652) (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in the Recombinant Bax Antibody (Ab090652) (1/400 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody was used a Goat Anti-Rabbit Alexa Fluor® 488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabeled sample (Black) was used as a control.
Recombinant Bax Antibody (Ab090652) - IP
Bax was immunoprecipitated from 0.5 mg HT-29 whole cell lysate with Recombinant Bax Antibody (Ab090652) at 1/25 dilution.
2nd Ab: GAR HRP for IP 1/500
Lane 1: Recombinant Bax Antibody (Ab090652) IP in HT-29 whole cell lysate
Lane 2: PBS instead of Recombinant Bax Antibody (Ab090652) in HT-29 whole cell lysate
Lane 3: HT-29 whole cell lysate, 10 μg (input)
Exposure: 30 s.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Application | Dilution info |
|---|---|
| Flow Cytometry |
1/100-1/400
|
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| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0404255 | Certificate of Analysis | Apr 09, 2024 | Ab090652 |
| ZJ23F0901011 | Certificate of Analysis | Sep 11, 2023 | Ab090652 |
| ZJ23F0901012 | Certificate of Analysis | Sep 11, 2023 | Ab090652 |
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