Recombinant; Rabbit anti Human BCL6 Antibody; WB, IHC, IF, ICC; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab090809-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $66.90 | |
| Ab090809-50μl | 50μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $189.90 | |
| Ab090809-100μl | 100μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $289.90 | |
| Ab090809-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,299.90 |
Recombinant; Rabbit anti Human BCL6 Antibody; WB, IHC, IF, ICC; Unconjugated
| Product Name | Recombinant BCL6 Antibody - Primary antibody, specific to Bcl6, Rabbit IgG |
|---|---|
| Synonyms | B cell CLL/lymphoma 6 antibody; B cell lymphoma 6 protein antibody; B-cell lymphoma 5 protein antibody; B-cell lymphoma 6 protein antibody; BCL 5 antibody; Bcl 6 antibody; BCL-5 antibody; BCL-6 antibody; BCL5 antibody; BCL6 antibody; BCL6_HUMAN antibody; |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.9 mg/mL |
| Host species | Rabbit |
| Specificity | Bcl6 |
| Immunogen | A synthetic peptide derived from human BCL6 (AA 687-706). |
| Positive Control | WB: Raji and Ramos cell lysates. IHC: Human tonsil. ICC/IF: Ramos cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description |
Rabbit anti Human BCL6 Antibody, Recombinant, could be used for WB, IHC, IF, ICC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 0.9 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant BCL6 Antibody (Ab090809) - Western Blot
All lanes: Recombinant BCL6 Antibody (Ab090809) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 79 kDa
Observed band size: 81 kDa
Exposure time: 60.0 s
Recombinant BCL6 Antibody (Ab090809) - IF/ICC
Immunofluorescence analysis of BCL6 in Ramos cells using Recombinant BCL6 Antibody (Ab090809).
Recombinant BCL6 Antibody (Ab090809) - IHC
Immunohistochemistry analysis of paraffin-embedded Human tonsil using Recombinant BCL6 Antibody (Ab090809). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/2000 |
| IHC | 1/50 - 1/100 |
| IF/ICC | 1/50 - 1/200 |
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F1001290 | Certificate of Analysis | Oct 13, 2023 | Ab090809 |
| ZJ23F1001291 | Certificate of Analysis | Oct 13, 2023 | Ab090809 |
| 1. Shaffer AL, Yu X, He Y, Boldrick J, Chan EP, Staudt LM. (2000) BCL-6 represses genes that function in lymphocyte differentiation, inflammation, and cell cycle control.. Immunity, 13 (2): (199-212). [PMID:10981963] |
| 2. Ghetu AF, Corcoran CM, Cerchietti L, Bardwell VJ, Melnick A, Privé GG. (2008) Structure of a BCOR corepressor peptide in complex with the BCL6 BTB domain dimer.. Mol Cell, 29 (3): (384-91). [PMID:18280243] |
| 3. Ahmad KF, Melnick A, Lax S, Bouchard D, Liu J, Kiang CL, Mayer S, Takahashi S, Licht JD, Privé GG. (2003) Mechanism of SMRT corepressor recruitment by the BCL6 BTB domain.. Mol Cell, 12 (6): (1551-64). [PMID:14690607] |
| 4. Yasui T, Yamamoto T, Sakai N, Asano K, Takai T, Yoshitomi Y, Davis M, Takagi T, Sakamoto K, Sogabe S et al.. (2017) Discovery of a novel B-cell lymphoma 6 (BCL6)-corepressor interaction inhibitor by utilizing structure-based drug design.. Bioorg Med Chem, 25 (17): (4876-4886). [PMID:28760529] |
| 5. Cardenas MG, Yu W, Beguelin W, Teater MR, Geng H, Goldstein RL, Oswald E, Hatzi K, Yang SN, Cohen J et al.. (2016) Rationally designed BCL6 inhibitors target activated B cell diffuse large B cell lymphoma.. J Clin Invest, 126 (9): (3351-62). [PMID:27482887] |
| 6. Cerchietti LC, Ghetu AF, Zhu X, Da Silva GF, Zhong S, Matthews M, Bunting KL, Polo JM, Farès C, Arrowsmith CH et al.. (2010) A small-molecule inhibitor of BCL6 kills DLBCL cells in vitro and in vivo.. Cancer Cell, 17 (4): (400-11). [PMID:20385364] |
| 7. Kerres N, Steurer S, Schlager S, Bader G, Berger H, Caligiuri M, Dank C, Engen JR, Ettmayer P, Fischerauer B et al.. (2017) Chemically Induced Degradation of the Oncogenic Transcription Factor BCL6.. Cell Rep, 20 (12): (2860-2875). [PMID:28930682] |
| 8. McCoull W, Cheung T, Anderson E, Barton P, Burgess J, Byth K, Cao Q, Castaldi MP, Chen H, Chiarparin E et al.. (2018) Development of a Novel B-Cell Lymphoma 6 (BCL6) PROTAC To Provide Insight into Small Molecule Targeting of BCL6.. ACS Chem Biol, 13 (11): (3131-3141). [PMID:30335946] |
| 9. McCoull W, Abrams RD, Anderson E, Blades K, Barton P, Box M, Burgess J, Byth K, Cao Q, Chuaqui C, Carbajo RJ, Cheung T, Code E, Ferguson AD, Fillery S, Fuller NO, Gangl E, Gao N, Grist M, Hargreaves D, Howard MR, Hu J, Kemmitt PD, Nelson JE, O'Connell N, Prince DB, Raubo P, Rawlins PB, Robb GR, Shi J, Waring MJ, Whittaker D, Wylot M, Zhu X.. (2017) Discovery of Pyrazolo[1,5-a]pyrimidine B-Cell Lymphoma 6 (BCL6) Binders and Optimization to High Affinity Macrocyclic Inhibitors.. J Med Chem, 60 (10): (4386-4402). [PMID:28485934] |
| 10. Kamada Y, Sakai N, Sogabe S, Ida K, Oki H, Sakamoto K, Lane W, Snell G, Iida M, Imaeda Y, Sakamoto J, Matsui J.. (2017) Discovery of a B-Cell Lymphoma 6 Protein-Protein Interaction Inhibitor by a Biophysics-Driven Fragment-Based Approach.. J Med Chem, 60 (10): (4358-4368). [PMID:28471657] |
| 11. Cheng H, Linhares BM, Yu W, Cardenas MG, Ai Y, Jiang W, Winkler A, Cohen S, Melnick A, MacKerell A, Cierpicki T, Xue F.. (2018) Identification of Thiourea-Based Inhibitors of the B-Cell Lymphoma 6 BTB Domain via NMR-Based Fragment Screening and Computer-Aided Drug Design.. J Med Chem, 61 (17): (7573-7588). [PMID:29969259] |
| 12. Bellenie BR, Cheung KJ, Varela A, Pierrat OA, Collie GW, Box GM, Bright MD, Gowan S, Hayes A, Rodrigues MJ, Shetty KN, Carter M, Davis OA, Henley AT, Innocenti P, Johnson LD, Liu M, de Klerk S, Le Bihan YV, Lloyd MG, McAndrew PC, Shehu E, Talbot R, Woodward HL, Burke R, Kirkin V, van Montfort RLM, Raynaud FI, Rossanese OW, Hoelder S.. (2020) Achieving In Vivo Target Depletion through the Discovery and Optimization of Benzimidazolone BCL6 Degraders.. J Med Chem, 63 (8): (4047-4068). [PMID:32275432] |
| 13. Guo W, Xing Y, Zhang Q, Xie J, Huang D, Gu H, He P, Zhou M, Xu S, Pang X, Liu M, Yi Z, Chen Y.. (2020) Synthesis and Biological Evaluation of B-Cell Lymphoma 6 Inhibitors of N-Phenyl-4-pyrimidinamine Derivatives Bearing Potent Activities against Tumor Growth.. J Med Chem, 63 (2): (676-695). [PMID:31895575] |
| 14. Teng M, Ficarro SB, Yoon H, Che J, Zhou J, Fischer ES, Marto JA, Zhang T, Gray NS.. (2020) Rationally Designed Covalent BCL6 Inhibitor That Targets a Tyrosine Residue in the Homodimer Interface.. ACS Med Chem Lett, 11 (6): (1269-1273). [PMID:32551010] |
| 15. Kerckaert, J P JP and 5 more authors.. (1993) LAZ3, a novel zinc-finger encoding gene, is disrupted by recurring chromosome 3q27 translocations in human lymphomas.. Nature genetics, [PMID:8220427] |
| 16. Ye, B H BH and 6 more authors.. (1993) Alterations of a zinc finger-encoding gene, BCL-6, in diffuse large-cell lymphoma.. Science (New York, N.Y.), (29): [PMID:8235596] |
| 17. Miki, T T, Kawamata, N N, Hirosawa, S S and Aoki, N N.. (1994) Gene involved in the 3q27 translocation associated with B-cell lymphoma, BCL5, encodes a Krüppel-like zinc-finger protein.. Blood, (1): [PMID:8274740] |
| 18. Baron, B W BW and 5 more authors.. (1993) Identification of the gene associated with the recurring chromosomal translocations t(3;14)(q27;q32) and t(3;22)(q27;q11) in B-cell lymphomas.. Proceedings of the National Academy of Sciences of the United States of America, (1): [PMID:8506375] |
| 19. Niu, H H, Ye, B H BH and Dalla-Favera, R R.. (1998) Antigen receptor signaling induces MAP kinase-mediated phosphorylation and degradation of the BCL-6 transcription factor.. Genes & development, (1): [PMID:9649500] |
| 20. Galiègue-Zouitina, S S and 6 more authors.. (1999) Nonrandom fusion of L-plastin(LCP1) and LAZ3(BCL6) genes by t(3;13)(q27;q14) chromosome translocation in two cases of B-cell non-Hodgkin lymphoma.. Genes, chromosomes & cancer, [PMID:10469447] |
| 21. Hosokawa, Y Y and 5 more authors.. (2000) The Ikaros gene, a central regulator of lymphoid differentiation, fuses to the BCL6 gene as a result of t(3;7)(q27;p12) translocation in a patient with diffuse large B-cell lymphoma.. Blood, (15): [PMID:10753856] |
| 22. Ueda, Chiyoko C and 8 more authors.. (2002) The gene for interleukin-21 receptor is the partner of BCL6 in t(3;16)(q27;p11), which is recurrently observed in diffuse large B-cell lymphoma.. Oncogene, (17): [PMID:11821949] |
| 23. Bereshchenko, Oksana R OR, Gu, Wei W and Dalla-Favera, Riccardo R.. (2002) Acetylation inactivates the transcriptional repressor BCL6.. Nature genetics, [PMID:12402037] |
| 24. Kurata, Masayuki M and 6 more authors.. (2002) Characterization of t(3;6)(q27;p21) breakpoints in B-cell non-Hodgkin's lymphoma and construction of the histone H4/BCL6 fusion gene, leading to altered expression of Bcl-6.. Cancer research, (1): [PMID:12414651] |
| 25. Mascle, Xavier X, Albagli, Olivier O and Lemercier, Claudie C.. (2003) Point mutations in BCL6 DNA-binding domain reveal distinct roles for the six zinc fingers.. Biochemical and biophysical research communications, (10): [PMID:12504096] |
| 26. Petrie, Kevin K and 6 more authors.. (2003) The histone deacetylase 9 gene encodes multiple protein isoforms.. The Journal of biological chemistry, (2): [PMID:12590135] |
| 27. Fujita, Naoyuki N and 6 more authors.. (2004) MTA3 and the Mi-2/NuRD complex regulate cell fate during B lymphocyte differentiation.. Cell, (1): [PMID:15454082] |
| 28. Phan, Ryan T RT and Dalla-Favera, Riccardo R.. (2004) The BCL6 proto-oncogene suppresses p53 expression in germinal-centre B cells.. Nature, (2): [PMID:15577913] |
| 29. Phan, Ryan T RT, Saito, Masumichi M, Basso, Katia K, Niu, Huifeng H and Dalla-Favera, Riccardo R.. (2005) BCL6 interacts with the transcription factor Miz-1 to suppress the cyclin-dependent kinase inhibitor p21 and cell cycle arrest in germinal center B cells.. Nature immunology, [PMID:16142238] |
| 30. Pantano, Serafino S, Jarrossay, David D, Saccani, Simona S, Bosisio, Daniela D and Natoli, Gioacchino G.. (2006) Plastic downregulation of the transcriptional repressor BCL6 during maturation of human dendritic cells.. Experimental cell research, (1): [PMID:16455075] |
| 31. Muzny, Donna M DM and 113 more authors.. (2006) The DNA sequence, annotation and analysis of human chromosome 3.. Nature, (27): [PMID:16641997] |
| 32. Phan, Ryan T RT, Saito, Masumichi M, Kitagawa, Yukiko Y, Means, Anthony R AR and Dalla-Favera, Riccardo R.. (2007) Genotoxic stress regulates expression of the proto-oncogene Bcl6 in germinal center B cells.. Nature immunology, [PMID:17828269] |
| 33. Mendez, Lourdes M LM and 6 more authors.. (2008) CtBP is an essential corepressor for BCL6 autoregulation.. Molecular and cellular biology, [PMID:18212045] |
| 34. Stead, Mark A MA and 5 more authors.. (2008) Structure of the wild-type human BCL6 POZ domain.. Acta crystallographica. Section F, Structural biology and crystallization communications, (1): [PMID:19052359] |
| 35. Duan, Shanshan S and 9 more authors.. (2012) FBXO11 targets BCL6 for degradation and is inactivated in diffuse large B-cell lymphomas.. Nature, (5): [PMID:22113614] |
| 36. Basso, Katia K and 6 more authors.. (2012) BCL6 positively regulates AID and germinal center gene expression via repression of miR-155.. The Journal of experimental medicine, (17): [PMID:23166356] |
| 37. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
| 38. Hatzi, Katerina K and 19 more authors.. (2013) A hybrid mechanism of action for BCL6 in B cells defined by formation of functionally distinct complexes at enhancers and promoters.. Cell reports, (15): [PMID:23911289] |
| 39. Mena, Elijah L EL and 7 more authors.. (2018) Dimerization quality control ensures neuronal development and survival.. Science (New York, N.Y.), (12): [PMID:30190310] |
