Recombinant (RR607); Rabbit anti Human Beta III Tubulin Antibody; WB, Flow; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab091154-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $49.90 | |
| Ab091154-50μl | 50μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $147.90 | |
| Ab091154-100μl | 100μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $237.90 | |
| Ab091154-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $1,707.90 |
Recombinant (RR607); Rabbit anti Human Beta III Tubulin Antibody; WB, Flow; Unconjugated
| Product Name | Recombinant beta III Tubulin Antibody - Primary antibody, specific to TUBB3, Rabbit IgG |
|---|---|
| Synonyms | beta 3 tubulin antibody; beta 4 antibody; beta-4 antibody; CDCBM antibody; CDCBM1 antibody; CFEOM3 antibody; CFEOM3A antibody; FEOM3 antibody; M(beta)3 antibody; M(beta)6 antibody; MC1R antibody; Neuron specific beta III Tubulin antibody; Neuron-specific |
| Specifications & Purity | ExactAb™, Validated, Recombinant, High performance, 0.088 mg/mL |
| Host species | Rabbit |
| Specificity | TUBB3 |
| Immunogen | A synthetic peptide corresponding to residues on the C-terminus of human beta III tubulin was used as an immunogen. |
| Positive Control | WB: Jurkat, HeLa, NIH/3T3, C6, HEK293, HUVEC and A549 cell lysates. Flow: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, High Performance, Recombinant, Validated |
| Product Description | Rabbit anti Human Beta III Tubulin Antibody, Recombinant (RR607), could be used for WB, Flow and so on. Application: WB: 1/2000 - 1/5000 Flow: 1/200 - 1/1000 Protein Function Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance. Post-translational Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 0.088 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant beta III Tubulin Antibody (Ab091154) - Western Blot
All lanes: Recombinant beta III Tubulin Antibody (Ab091154) at 1/5000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 50 kDa
Observed band size: 51 kDa
Exposure time: 6.3 s
Recombinant beta III Tubulin Antibody (Ab091154) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Recombinant beta III Tubulin Antibody (Ab091154) (gold). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in Recombinant beta III Tubulin Antibody (Ab091154) (1/1000 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody was used a Goat Anti-Rabbit Alexa Fluor® 488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabeled sample (Red) was used as a control.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Application | Dilution info |
|---|---|
| Flow Cytometry |
1/200 - 1/1000
|
| WB |
1/2000 - 1/5000
|
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F0901098 | Certificate of Analysis | Sep 18, 2023 | Ab091154 |
| ZJ23F0901099 | Certificate of Analysis | Sep 18, 2023 | Ab091154 |
