Recombinant beta Tubulin Antibody - Primary antibody, specific to TUBB, Rabbit IgG

    Application:
  • IF/ICC
  • IHC
  • WB
Features and benefits
  • Host species: Rabbit
  • Species reactivity(Reacts with): Human,Mouse,Rat
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
Item Number
Ab091197
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab091197-10μl
10μl
In stock
$49.90
Ab091197-50μl
50μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$147.90
Ab091197-100μl
100μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$207.90
Ab091197-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,617.90

Recombinant; Rabbit anti Human Beta Tubulin Antibody; WB, IHC, ICC, IF; Unconjugated

Basic Description

Product NameRecombinant beta Tubulin Antibody - Primary antibody, specific to TUBB, Rabbit IgG
SynonymsBeta 4 tubulin antibody | Beta 5 tubulin antibody | beta Ib tubulin antibody | Beta1 tubulin antibody | Class I beta tubulin antibody | M40 antibody | MGC117247 antibody | MGC16435 antibody | OK/SW cl.56 antibody | OK/SWcl.56 antibody | TBB5_HUMAN antibod
Specifications & PurityExactAb™, Validated, Recombinant, 0.2 mg/mL
Host speciesRabbit
SpecificityTUBB
ImmunogenA synthetic peptide derived from human beta Tubulin (AA 324-337).
Positive ControlWB: K562, C6, NIH3T3, HeLa, A431, Jurkat, HEK293 and MCF7 cell lysates. IHC: Human brain tissues. IF/ICC: HeLa cells.
ConjugationUnconjugated
GradeExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human Beta Tubulin Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on.
Application:
WB: 1/500 - 1/1000
IHC: 1/50-1/200
IF/ICC: 1/100 - 1/200
Protein Function
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Post-translational
Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).
Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear.
Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.

Product Properties

IsotypeRabbit IgG
SDS-PAGE150 kDa
Purification MethodAntigen affinity purified
FormLiquid
Concentration0.2 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant beta Tubulin Antibody (Ab091197) - Western Blot
All lanes: Recombinant beta Tubulin Antibody (Ab091197) at 1/5000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution

Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 15.0 s


Recombinant beta Tubulin Antibody (Ab091197) - ICC/IF
Immunocytochemistry analysis of beta Tubulin(green) in HeLa cells using Recombinant beta Tubulin Antibody (Ab091197) and cells were counterstained with DAPI (blue).

Recombinant beta Tubulin Antibody (Ab091197) - IHC
Immunohistochemistry analysis of paraffin-embedded Human brain using Recombinant beta Tubulin Antibody (Ab091197) at 1/50 dilution. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Associated Targets(Human)

TUBB Tclin Tubulin beta chain (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Mechanisms of Action

Mechanism of ActionAction Typetarget IDTarget NameTarget TypeTarget OrganismBinding Site NameReferences

Application

ApplicationDilution info
WB1/500-1/1000

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1/500-1/1000

IHC1/50-1/200

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1/50-1/200

Certificates

Certificate of Analysis(COA)

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3 results found

Lot NumberCertificate TypeDateItem
ZJ24F0404259Certificate of AnalysisApr 09, 2024 Ab091197
ZJ23F0800811Certificate of AnalysisAug 16, 2023 Ab091197
ZJ23F0800812Certificate of AnalysisAug 16, 2023 Ab091197

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