Recombinant; Rabbit anti Human BOB1 Antibody; WB; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab091495-10μl | 10μl | In stock | $69.90 | |
| Ab091495-50μl | 50μl | In stock | $199.90 | |
| Ab091495-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $309.90 | |
| Ab091495-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,699.90 |
Recombinant; Rabbit anti Human BOB1 Antibody; WB; Unconjugated
| Product Name | Recombinant BOB1 Antibody - Primary antibody, specific to POU2AF1, Rabbit IgG |
|---|---|
| Synonyms | B-cell oct-binding protein 1 | B-cell-specific coactivator OBF-1 | POU domain class 2-associating factor 1 | POU2AF1 | OCT-binding factor 1 | BOB-1 | OCA-B. OBF1 | OCA-B | OBF1_HUMAN | BOB.1. POU domain class 2-associating factor 1 | POU2AF1 (BOB1, OBF1) |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.37 mg/mL |
| Host species | Rabbit |
| Specificity | POU2AF1 |
| Immunogen | A synthetic peptide derived from human BOB1 (AA 180-256) |
| Positive Control | WB: Ramos and Raji cell lysates. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human BOB1 Antibody, Recombinant, could be used for WB and so on. |
| Isotype | Rabbit IgG |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 0.37 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant BOB1 Antibody (Ab091495) - Western Blot
All lanes: Recombinant BOB1 Antibody (Ab091495) at 1/500 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 27 kDa
Observed band size: 32 kDa
Exposure time: 195.1 s
Recombinant BOB1 Antibody (Ab091495) - Western Blot
All lanes: Recombinant BOB1 Antibody (Ab091495) at 1/500 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/5000 dilution
Predicted band size: 27 kDa
Observed band size: 32 kDa
Exposure time: 78.2 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0505885 | Certificate of Analysis | May 21, 2024 | Ab091495 |
| ZJ24F0505884 | Certificate of Analysis | May 21, 2024 | Ab091495 |
| 1. Luo, Y Y and Roeder, R G RG.. (1995) Cloning, functional characterization, and mechanism of action of the B-cell-specific transcriptional coactivator OCA-B.. Molecular and cellular biology, [PMID:7623806] |
| 2. Gstaiger, M M, Knoepfel, L L, Georgiev, O O, Schaffner, W W and Hovens, C M CM.. (1995) A B-cell coactivator of octamer-binding transcription factors.. Nature, (26): [PMID:7779176] |
| 3. Strubin, M M, Newell, J W JW and Matthias, P P.. (1995) OBF-1, a novel B cell-specific coactivator that stimulates immunoglobulin promoter activity through association with octamer-binding proteins.. Cell, (10): [PMID:7859290] |
| 4. Galiègue-Zouitina, S S and 9 more authors.. (1995) Fusion of the LAZ3/BCL6 and BOB1/OBF1 genes by t(3; 11) (q27; q23) chromosomal translocation.. Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie, [PMID:8574789] |
| 5. Chasman, D D, Cepek, K K, Sharp, P A PA and Pabo, C O CO.. (1999) Crystal structure of an OCA-B peptide bound to an Oct-1 POU domain/octamer DNA complex: specific recognition of a protein-DNA interface.. Genes & development, (15): [PMID:10541551] |
| 6. Tiedt, R R, Bartholdy, B A BA, Matthias, G G, Newell, J W JW and Matthias, P P.. (2001) The RING finger protein Siah-1 regulates the level of the transcriptional coactivator OBF-1.. The EMBO journal, (1): [PMID:11483517] |
| 7. Boehm, J J, He, Y Y, Greiner, A A, Staudt, L L and Wirth, T T.. (2001) Regulation of BOB.1/OBF.1 stability by SIAH.. The EMBO journal, (1): [PMID:11483518] |
