Recombinant; Rabbit anti Human c-Myc Antibody; WB, IF, ICC; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab097003-10μl | 10μl | In stock | $66.90 | |
| Ab097003-50μl | 50μl | In stock | $199.90 | |
| Ab097003-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $239.90 | |
| Ab097003-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,199.90 |
Recombinant; Rabbit anti Human c-Myc Antibody; WB, IF, ICC; Unconjugated
| Product Name | Recombinant c-Myc Antibody - Primary antibody, specific to MYC, Rabbit IgG |
|---|---|
| Synonyms | MYC; BHLHE39; Myc proto-oncogene protein; Class E basic helix-loop-helix protein 39; bHLHe39; Proto-oncogene c-Myc; Transcription factor p64; AU016757 antibody; Avian myelocytomatosis viral oncogene homolog antibody; bHLHe39 antibody; c Myc antibody; Cell |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.3 mg/mL |
| Host species | Rabbit |
| Specificity | MYC |
| Immunogen | A synthetic peptide derived from human c-Myc (AA 16-35) |
| Positive Control | WB: K562, Jurkat, LNCaP, HeLa and HT-29 cell lysates. ICC/IF: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human c-Myc Antibody, Recombinant, could be used for WB, IF, ICCand so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Immunogen affinity purified |
| Form | Liquid |
| Concentration | 0.3 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant c-Myc Antibody (Ab097003) - Western Blot
All lanes: Recombinant c-Myc Antibody (Ab097003) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 55 kDa
Exposure time: 8.5 s
Recombinant c-Myc Antibody (Ab097003) - IF/ICC
Immunocytochemistry analysis of c-Myc (green) in HeLa using Recombinant c-Myc Antibody (Ab097003), and cells were counterstained with DAPI (blue).
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
| ICC/IF |
1/50-1/200
|
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0102608 | Certificate of Analysis | Jan 25, 2024 | Ab097003 |
| ZJ24F0102607 | Certificate of Analysis | Jan 25, 2024 | Ab097003 |
| 1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M. (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.. Science, 325 (5942): (834-40). [PMID:19608861] |
| 2. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330] |
| 3. Yap JL, Wang H, Hu A, Chauhan J, Jung KY, Gharavi RB, Prochownik EV, Fletcher S.. (2013) Pharmacophore identification of c-Myc inhibitor 10074-G5.. Bioorg Med Chem Lett, 23 (1): (370-374). [PMID:23177256] |
| 4. Zaki MA, Balachandran P, Khan S, Wang M, Mohammed R, Hetta MH, Pasco DS, Muhammad I.. (2013) Cytotoxicity and modulation of cancer-related signaling by (Z)- and (E)-3,4,3',5'-tetramethoxystilbene isolated from Eugenia rigida.. J Nat Prod, 76 (4): (679-684). [PMID:23547843] |
| 5. Jung KY, Wang H, Teriete P, Yap JL, Chen L, Lanning ME, Hu A, Lambert LJ, Holien T, Sundan A, Cosford ND, Prochownik EV, Fletcher S.. (2015) Perturbation of the c-Myc-Max protein-protein interaction via synthetic α-helix mimetics.. J Med Chem, 58 (7): (3002-3024). [PMID:25734936] |
| 6. Fu DJ, Zhang L, Song J, Mao RW, Zhao RH, Liu YC, Hou YH, Li JH, Yang JJ, Jin CY, Li P, Zi XL, Liu HM, Zhang SY, Zhang YB.. (2017) Design and synthesis of formononetin-dithiocarbamate hybrids that inhibit growth and migration of PC-3 cells via MAPK/Wnt signaling pathways.. Eur J Med Chem, 127 (87-99). [PMID:28038329] |
| 7. Wiedemann B, Weisner J, Rauh D.. (2018) Chemical modulation of transcription factors.. Medchemcomm, 9 (8): (1249-1272). [PMID:30151079] |
| 8. Carabet LA, Lallous N, Leblanc E, Ban F, Morin H, Lawn S, Ghaidi F, Lee J, Mills IG, Gleave ME, Rennie PS, Cherkasov A.. (2018) Computer-aided drug discovery of Myc-Max inhibitors as potential therapeutics for prostate cancer.. Eur J Med Chem, 160 (108-119). [PMID:30326371] |
| 9. Chen L,Cheng B,Sun Q,Lai L. (2021) Ligand-based optimization and biological evaluation of N-(2,2,2-trichloro-1-(3-phenylthioureido)ethyl)acetamide derivatives as potent intrinsically disordered protein c-Myc inhibitors.. Bioorg Med Chem Lett, 31 (127711-127711). [PMID:33246106] |
| 10. Iijima, S S, Teraoka, H H, Date, T T and Tsukada, K K.. (1992) DNA-activated protein kinase in Raji Burkitt's lymphoma cells. Phosphorylation of c-Myc oncoprotein.. European journal of biochemistry, (1): [PMID:1597196] |
| 11. Rimokh, R R and 9 more authors.. (1991) A chromosome 12 coding region is juxtaposed to the MYC protooncogene locus in a t(8;12)(q24;q22) translocation in a case of B-cell chronic lymphocytic leukemia.. Genes, chromosomes & cancer, [PMID:2069907] |
| 12. Hann, S R SR, King, M W MW, Bentley, D L DL, Anderson, C W CW and Eisenman, R N RN.. (1988) A non-AUG translational initiation in c-myc exon 1 generates an N-terminally distinct protein whose synthesis is disrupted in Burkitt's lymphomas.. Cell, (29): [PMID:3277717] |
| 13. Bentley, D L DL and Groudine, M M.. (1986) Novel promoter upstream of the human c-myc gene and regulation of c-myc expression in B-cell lymphomas.. Molecular and cellular biology, [PMID:3540591] |
| 14. Colby, W W WW, Chen, E Y EY, Smith, D H DH and Levinson, A D AD.. (1983) Identification and nucleotide sequence of a human locus homologous to the v-myc oncogene of avian myelocytomatosis virus MC29.. Nature, (24): [PMID:6298632] |
| 15. Watt, R R and 5 more authors.. (1983) Nucleotide sequence of cloned cDNA of human c-myc oncogene.. Nature, [PMID:6304538] |
| 16. Watson, D K DK, Psallidopoulos, M C MC, Samuel, K P KP, Dalla-Favera, R R and Papas, T S TS.. (1983) Nucleotide sequence analysis of human c-myc locus, chicken homologue, and myelocytomatosis virus MC29 transforming gene reveals a highly conserved gene product.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:6304729] |
| 17. Bernard, O O, Cory, S S, Gerondakis, S S, Webb, E E and Adams, J M JM.. (1983) Sequence of the murine and human cellular myc oncogenes and two modes of myc transcription resulting from chromosome translocation in B lymphoid tumours.. The EMBO journal, [PMID:6321164] |
| 18. Battey, J J and 7 more authors.. (1983) The human c-myc oncogene: structural consequences of translocation into the IgH locus in Burkitt lymphoma.. Cell, [PMID:6414718] |
| 19. Rabbitts, T H TH, Hamlyn, P H PH and Baer, R R.. (1983) Altered nucleotide sequences of a translocated c-myc gene in Burkitt lymphoma.. Nature, [PMID:6419122] |
| 20. Rabbitts, T H TH, Forster, A A, Hamlyn, P P and Baer, R R.. (1984) Effect of somatic mutation within translocated c-myc genes in Burkitt's lymphoma.. Nature, [PMID:6547209] |
| 21. Gazin, C C and 6 more authors.. (1984) Nucleotide sequence of the human c-myc locus: provocative open reading frame within the first exon.. The EMBO journal, [PMID:6714223] |
| 22. Chou, T Y TY, Hart, G W GW and Dang, C V CV.. (1995) c-Myc is glycosylated at threonine 58, a known phosphorylation site and a mutational hot spot in lymphomas.. The Journal of biological chemistry, (11): [PMID:7642555] |
| 23. Bhatia, K K and 5 more authors.. (1993) Point mutations in the c-Myc transactivation domain are common in Burkitt's lymphoma and mouse plasmacytomas.. Nature genetics, [PMID:8220424] |
| 24. Gupta, S S, Seth, A A and Davis, R J RJ.. (1993) Transactivation of gene expression by Myc is inhibited by mutation at the phosphorylation sites Thr-58 and Ser-62.. Proceedings of the National Academy of Sciences of the United States of America, (15): [PMID:8386367] |
| 25. Tachibana, K K and 7 more authors.. (1993) Allele-specific activation of the c-myc gene in an atypical Burkitt's lymphoma carrying the t(2;8) chromosomal translocation 250 kb downstream from c-myc.. Gene, (28): [PMID:8444346] |
| 26. Alexandrov, I I, Shlyakhova, L L, Vartanian, A A, Zajac-Kaye, M M and Alexandrova, N N.. (1997) c-Raf kinase binds to N-terminal domain of c-Myc.. FEBS letters, (8): [PMID:9315742] |
| 27. Lavigne, P P and 5 more authors.. (1998) Insights into the mechanism of heterodimerization from the 1H-NMR solution structure of the c-Myc-Max heterodimeric leucine zipper.. Journal of molecular biology, (7): [PMID:9680483] |
| 28. Law, B K BK and 6 more authors.. (2000) Salicylate-induced growth arrest is associated with inhibition of p70s6k and down-regulation of c-myc, cyclin D1, cyclin A, and proliferating cell nuclear antigen.. The Journal of biological chemistry, (8): [PMID:10993886] |
| 29. Yada, Masayoshi M and 9 more authors.. (2004) Phosphorylation-dependent degradation of c-Myc is mediated by the F-box protein Fbw7.. The EMBO journal, (19): [PMID:15103331] |
| 30. Yu, Mei M and 15 more authors.. (2005) PARP-10, a novel Myc-interacting protein with poly(ADP-ribose) polymerase activity, inhibits transformation.. Oncogene, (17): [PMID:15674325] |
| 31. Grandori, Carla C and 6 more authors.. (2005) c-Myc binds to human ribosomal DNA and stimulates transcription of rRNA genes by RNA polymerase I.. Nature cell biology, [PMID:15723054] |
| 32. Zhang, Kangling K, Faiola, Francesco F and Martinez, Ernest E.. (2005) Six lysine residues on c-Myc are direct substrates for acetylation by p300.. Biochemical and biophysical research communications, (14): [PMID:16126174] |
| 33. Nusbaum, Chad C and 75 more authors.. (2006) DNA sequence and analysis of human chromosome 8.. Nature, (19): [PMID:16421571] |
| 34. Suzuki, Chie C and 8 more authors.. (2007) Identification of Myc-associated protein with JmjC domain as a novel therapeutic target oncogene for lung cancer.. Molecular cancer therapeutics, [PMID:17308053] |
| 35. Popov, Nikita N and 8 more authors.. (2007) The ubiquitin-specific protease USP28 is required for MYC stability.. Nature cell biology, [PMID:17558397] |
| 36. Junttila, Melissa R MR and 19 more authors.. (2007) CIP2A inhibits PP2A in human malignancies.. Cell, (13): [PMID:17632056] |
| 37. Popov, Nikita N, Herold, Steffi S, Llamazares, Maria M, Schülein, Christina C and Eilers, Martin M.. (2007) Fbw7 and Usp28 regulate myc protein stability in response to DNA damage.. Cell cycle (Georgetown, Tex.), (1): [PMID:17873522] |
| 38. Takahashi, Kazutoshi K and 6 more authors.. (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors.. Cell, (30): [PMID:18035408] |
| 39. Dephoure, Noah N and 6 more authors.. (2008) A quantitative atlas of mitotic phosphorylation.. Proceedings of the National Academy of Sciences of the United States of America, (5): [PMID:18669648] |
| 40. Hydbring, Per P and 14 more authors.. (2010) Phosphorylation by Cdk2 is required for Myc to repress Ras-induced senescence in cotransformation.. Proceedings of the National Academy of Sciences of the United States of America, (5): [PMID:19966300] |
| 41. David, Charles J CJ, Chen, Mo M, Assanah, Marcela M, Canoll, Peter P and Manley, James L JL.. (2010) HnRNP proteins controlled by c-Myc deregulate pyruvate kinase mRNA splicing in cancer.. Nature, (21): [PMID:20010808] |
| 42. Choi, Seung H SH, Wright, Jason B JB, Gerber, Scott A SA and Cole, Michael D MD.. (2010) Myc protein is stabilized by suppression of a novel E3 ligase complex in cancer cells.. Genes & development, (15): [PMID:20551172] |
| 43. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692] |
| 44. Taira, Naoe N and 6 more authors.. (2012) DYRK2 priming phosphorylation of c-Jun and c-Myc modulates cell cycle progression in human cancer cells.. The Journal of clinical investigation, [PMID:22307329] |
| 45. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
| 46. Shi, Yi Y and 7 more authors.. (2014) tRNA synthetase counteracts c-Myc to develop functional vasculature.. eLife, (17): [PMID:24940000] |
| 47. Cianfanelli, Valentina V and 21 more authors.. (2015) AMBRA1 links autophagy to cell proliferation and tumorigenesis by promoting c-Myc dephosphorylation and degradation.. Nature cell biology, [PMID:25438055] |
| 48. Sun, Xiao-Xin XX and 5 more authors.. (2015) The nucleolar ubiquitin-specific protease USP36 deubiquitinates and stabilizes c-Myc.. Proceedings of the National Academy of Sciences of the United States of America, (24): [PMID:25775507] |
| 49. Cianfanelli, Valentina V, D'Orazio, Melania M and Cecconi, Francesco F.. (2015) AMBRA1 and BECLIN 1 interplay in the crosstalk between autophagy and cell proliferation.. Cell cycle (Georgetown, Tex.), [PMID:25803737] |
| 50. Kim, Jee-Youn JY, Cho, Young-Eun YE and Park, Jae-Hoon JH.. (2015) The Nucleolar Protein GLTSCR2 Is an Upstream Negative Regulator of the Oncogenic Nucleophosmin-MYC Axis.. The American journal of pathology, [PMID:25956029] |
| 51. Ai, Guoqiang G, Dachineni, Rakesh R, Muley, Pratik P, Tummala, Hemachand H and Bhat, G Jayarama GJ.. (2016) Aspirin and salicylic acid decrease c-Myc expression in cancer cells: a potential role in chemoprevention.. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, [PMID:26314861] |
| 52. Cheng, Ran R and 14 more authors.. (2017) Aspirin regulation of c-myc and cyclinD1 proteins to overcome tamoxifen resistance in estrogen receptor-positive breast cancer cells.. Oncotarget, (2): [PMID:28415819] |
