| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab005519-10μg | 10μg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $29.90 | |
| Ab005519-100μg | 100μg | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $147.90 | |
| Ab005519-1mg | 1mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $1,107.90 |
Recombinant (27B9), Mouse anti Human CA153 Antibody; Capture antibody, ELISA, CLIA; Unconjugated
| Product Name | Recombinant CA153 Antibody - Primary antibody, for ELISA,CLIA,Capture antibody various applications, from Human, generated in Mouse, specific to Antibody recognizes MUC-1 core protein of cancer antigen 15-3 , IgG2b |
|---|---|
| Synonyms | Breast carcinoma-associated antigen DF3 | CA 15-3 | Cancer antigen 15-3 | Carcinoma-associated mucin | EMA | Episialin | H23AG | KL-6 | Krebs von den Lungen-6 | MUC-1 | Peanut-reactive urinary mucin | PEM | PEMT | Polymorphic epithelial mucin | PUM | Tumo |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Azide Free, Recombinant, Lot by Lot |
| Host Species | Mouse |
| Host species | Mouse |
| Specificity | Antibody recognizes MUC-1 core protein of cancer antigen 15-3 |
| Application | ELISA,CLIA,Capture antibody |
| Immunogen | Native protein |
| Epitope | 糖表位（类115D8） |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, Recombinant, Validated |
| Product Description | Recombinant (27B9), Mouse anti Human CA153 Antibody could be used as the capture antibody in ELISA, CLIA at 1.0 μg/mL. |
| Antibody Type | Primary antibody |
|---|---|
| Clonality | Recombinant |
| Isotype | IgG2b |
| Purification Method | Protein A or Protein G purified |
| Matched Pairs | [Ab116378] Recombinant CA153 Antibody |
| Source | CHO supernatant |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Storage: -20℃ for long term storage. Avoid repeated freeze / thaw cycles. |
Relevance of detection results between aladdin Antibody Pair (Ab005519 & Ab116378) and Roche in CLIA
· Capture antibody: Recombinant CA153 Antibody (Ab005519) at 1.0 μg/mL.
· Detection antibody: Recombinant CA153 Antibody (Ab116378) at 1.0 μg/mL.
· Linear Range: 0.5-200 U/mL, R² ≥ 0.92.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
| Application | Dilution info |
|---|---|
| ELISA |
1 μg/mL |
| CLIA |
1 μg/mL
|
| ELlSA |
1.0 ug/mL
|
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F0500189 | Certificate of Analysis | May 25, 2023 | Ab005519 |
| ZJ23F0500137 | Certificate of Analysis | May 17, 2023 | Ab005519 |
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| 17. Oosterkamp, H M HM, Scheiner, L L, Stefanova, M C MC, Lloyd, K O KO and Finstad, C L CL.. (1997) Comparison of MUC-1 mucin expression in epithelial and non-epithelial cancer cell lines and demonstration of a new short variant form (MUC-1/Z).. International journal of cancer, (3): [PMID:9212228] |
| 18. Müller, S S and 5 more authors.. (1997) Localization of O-glycosylation sites on glycopeptide fragments from lactation-associated MUC1. All putative sites within the tandem repeat are glycosylation targets in vivo.. The Journal of biological chemistry, (3): [PMID:9312074] |
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| 21. Müller, S S and 5 more authors.. (1999) High density O-glycosylation on tandem repeat peptide from secretory MUC1 of T47D breast cancer cells.. The Journal of biological chemistry, (25): [PMID:10373415] |
| 22. Li, Y Y, Kuwahara, H H, Ren, J J, Wen, G G and Kufe, D D.. (2001) The c-Src tyrosine kinase regulates signaling of the human DF3/MUC1 carcinoma-associated antigen with GSK3 beta and beta-catenin.. The Journal of biological chemistry, (2): [PMID:11152665] |
| 23. Parry, S S and 5 more authors.. (2001) Identification of MUC1 proteolytic cleavage sites in vivo.. Biochemical and biophysical research communications, (11): [PMID:11341784] |
| 24. Engelmann, K K, Baldus, S E SE and Hanisch, F G FG.. (2001) Identification and topology of variant sequences within individual repeat domains of the human epithelial tumor mucin MUC1.. The Journal of biological chemistry, (27): [PMID:11350974] |
| 25. Li, Y Y and 7 more authors.. (2001) The epidermal growth factor receptor regulates interaction of the human DF3/MUC1 carcinoma antigen with c-Src and beta-catenin.. The Journal of biological chemistry, (21): [PMID:11483589] |
| 26. Ren, Jian J, Li, Yongqing Y and Kufe, Donald D.. (2002) Protein kinase C delta regulates function of the DF3/MUC1 carcinoma antigen in beta-catenin signaling.. The Journal of biological chemistry, (17): [PMID:11877440] |
| 27. Thathiah, Amantha A, Blobel, Carl P CP and Carson, Daniel D DD.. (2003) Tumor necrosis factor-alpha converting enzyme/ADAM 17 mediates MUC1 shedding.. The Journal of biological chemistry, (31): [PMID:12441351] |
| 28. Li, Yongqing Y and 7 more authors.. (2003) Heregulin targets gamma-catenin to the nucleolus by a mechanism dependent on the DF3/MUC1 oncoprotein.. Molecular cancer research : MCR, [PMID:12939402] |
| 29. Huang, Lei L and 5 more authors.. () MUC1 cytoplasmic domain coactivates Wnt target gene transcription and confers transformation.. Cancer biology & therapy, [PMID:14688481] |
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| 31. Mukherjee, P P, Tinder, T L TL, Basu, G D GD and Gendler, S J SJ.. (2005) MUC1 (CD227) interacts with lck tyrosine kinase in Jurkat lymphoma cells and normal T cells.. Journal of leukocyte biology, [PMID:15513966] |
| 32. Levitin, Fiana F and 19 more authors.. (2005) A novel protein derived from the MUC1 gene by alternative splicing and frameshifting.. The Journal of biological chemistry, (18): [PMID:15623537] |
| 33. Wei, Xiaolong X, Xu, Hai H and Kufe, Donald D.. (2005) Human MUC1 oncoprotein regulates p53-responsive gene transcription in the genotoxic stress response.. Cancer cell, [PMID:15710329] |
| 34. Zhang, Li-Xin LX, Li, Chun-Hai CH, Sun, Li-Ya LY, Wang, Miao M and Lu, Hao-Jun HJ.. (2003) [Soluble expression of peptide containing MUC1/Y-specific epitope in Escherichia coli and preparation of the antibody].. Sheng wu gong cheng xue bao = Chinese journal of biotechnology, [PMID:15969018] |
| 35. Engelmann, Katja K and 6 more authors.. (2005) Transmembrane and secreted MUC1 probes show trafficking-dependent changes in O-glycan core profiles.. Glycobiology, [PMID:15972891] |
| 36. Levitin, Fiana F and 8 more authors.. (2005) The MUC1 SEA module is a self-cleaving domain.. The Journal of biological chemistry, (30): [PMID:15987679] |
| 37. Huang, Lei L and 5 more authors.. (2005) MUC1 oncoprotein blocks glycogen synthase kinase 3beta-mediated phosphorylation and degradation of beta-catenin.. Cancer research, (15): [PMID:16288032] |
| 38. Wei, Xiaolong X, Xu, Hai H and Kufe, Donald D.. (2006) MUC1 oncoprotein stabilizes and activates estrogen receptor alpha.. Molecular cell, (20): [PMID:16427018] |
| 39. Parry, Simon S and 6 more authors.. (2006) N-Glycosylation of the MUC1 mucin in epithelial cells and secretions.. Glycobiology, [PMID:16585136] |
| 40. Gregory, S G SG and 178 more authors.. (2006) The DNA sequence and biological annotation of human chromosome 1.. Nature, (18): [PMID:16710414] |
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| 42. Wei, Xiaolong X, Xu, Hai H and Kufe, Donald D.. (2007) Human mucin 1 oncoprotein represses transcription of the p53 tumor suppressor gene.. Cancer research, (15): [PMID:17308127] |
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| 46. Zhang, Lixin L, Vlad, Anda A, Milcarek, Christine C and Finn, Olivera J OJ.. (2013) Human mucin MUC1 RNA undergoes different types of alternative splicing resulting in multiple isoforms.. Cancer immunology, immunotherapy : CII, [PMID:22941036] |
| 47. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
| 48. Kirby, Andrew A and 42 more authors.. (2013) Mutations causing medullary cystic kidney disease type 1 lie in a large VNTR in MUC1 missed by massively parallel sequencing.. Nature genetics, [PMID:23396133] |
