Recombinant Caldesmon/CDM Antibody - Primary antibody, specific to Caldesmon/CDM, Rabbit IgG

    Application:
  • IHC
  • WB
Features and benefits
  • Host species: Rabbit
  • Species reactivity(Reacts with): Human,Mouse,Rat
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
Item Number
Ab092465
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab092465-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$66.90
Ab092465-50μl
50μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$199.90
Ab092465-100μl
100μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$339.90
Ab092465-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,399.90

Recombinant; Rabbit anti Human Caldesmon/CDM Antibody; WB, IHC; Unconjugated

Basic Description

Product NameRecombinant Caldesmon/CDM Antibody - Primary antibody, specific to Caldesmon/CDM, Rabbit IgG
SynonymsCAD antibody | CALD 1 antibody | CALD1 antibody | CALD1_HUMAN antibody | Caldesmon 1 antibody | Caldesmon 1 Isoform 1 antibody | Caldesmon 1 Isoform 2 antibody | Caldesmon 1 Isoform 3 antibody | Caldesmon 1 Isoform 4 antibody | Caldesmon 1 Isoform 5 antib
Specifications & PurityExactAb™, Validated, Recombinant, 0.2 mg/mL
Host speciesRabbit
SpecificityCaldesmon/CDM
ImmunogenA synthetic peptide derived from human Caldesmon (AA 782-793).
Positive ControlWB: C6, NIH/3T3, HeLa and HUVEC cell lysates. IHC: Human colon cancer tissue.
ConjugationUnconjugated
GradeExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human Caldesmon/CDM Antibody, Recombinant, could be used for WB, IHC and so on.
Application:
WB: 1/500 – 1/1000
IHC: 1/50-/100
Protein Function
Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also play an essential role during cellular mitosis and receptor capping.

Product Properties

IsotypeRabbit IgG
SDS-PAGE150 kDa
Purification MethodAntigen affinity purified
FormLiquid
Concentration0.2 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant Caldesmon/CDM Antibody (Ab092465) - Western Blot
All lanes: Recombinant Caldesmon/CDM Antibody (Ab092465) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 93 kDa
Observed band size: 76 kDa
Exposure time: 9.0 s

Recombinant Caldesmon/CDM Antibody (Ab092465) - IHC
Immunohistochemistry analysis of paraffin-embedded Human colon cancer using Recombinant Caldesmon/CDM Antibody (Ab092465). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Associated Targets(Human)

CALD1 Tbio Caldesmon (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
OR1C1 Tdark Olfactory receptor 1C1 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
WB1/500-1/1000

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1/500-1/1000

IHC1/50 - 1/100

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1/50 - 1/100

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot NumberCertificate TypeDateItem
ZJ23F0901181Certificate of AnalysisOct 07, 2023 Ab092465
ZJ23F0901182Certificate of AnalysisOct 07, 2023 Ab092465

Related Documents

References

1. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed.  (2009-05-06)  Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach..  Analytical chemistry,  81  ((11)): (4493-4501).  [PMID:19413330]
2. Hayashi, K K and 8 more authors..  (1992)  Genomic structure of the human caldesmon gene..  Proceedings of the National Academy of Sciences of the United States of America,    (15):   [PMID:1465449]
3. Humphrey, M B MB, Herrera-Sosa, H H, Gonzalez, G G, Lee, R R and Bryan, J J..  (1992)  Cloning of cDNAs encoding human caldesmons..  Gene,    (15):   [PMID:1555769]
4. Novy, R E RE, Lin, J L JL and Lin, J J JJ..  (1991)  Characterization of cDNA clones encoding a human fibroblast caldesmon isoform and analysis of caldesmon expression in normal and transformed cells..  The Journal of biological chemistry,    (5):   [PMID:1885618]
5. Hillier, Ladeana W LW and 106 more authors..  (2003)  The DNA sequence of human chromosome 7..  Nature,    (10):   [PMID:12853948]
6. Yu, Li-Rong LR and 5 more authors..  (2007)  Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra..  Journal of proteome research,      [PMID:17924679]
7. Dephoure, Noah N and 6 more authors..  (2008)  A quantitative atlas of mitotic phosphorylation..  Proceedings of the National Academy of Sciences of the United States of America,    (5):   [PMID:18669648]
8. Rigbolt, Kristoffer T G KT and 9 more authors..  (2011)  System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation..  Science signaling,    (15):   [PMID:21406692]
9. Zhou, Houjiang H and 6 more authors..  (2013)  Toward a comprehensive characterization of a human cancer cell phosphoproteome..  Journal of proteome research,    (4):   [PMID:23186163]
10. Bian, Yangyang Y and 9 more authors..  (2014)  An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome..  Journal of proteomics,    (16):   [PMID:24275569]
11. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge.  (2011-01-29)  Initial characterization of the human central proteome..  BMC systems biology,  (17-17).  [PMID:21269460]

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