A synthetic peptide derived from human Caldesmon (AA 782-793).
Positive Control
WB: C6, NIH/3T3, HeLa and HUVEC cell lysates. IHC: Human colon cancer tissue.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human Caldesmon/CDM Antibody, Recombinant, could be used for WB, IHC and so on. Application: WB: 1/500 – 1/1000 IHC: 1/50-/100 Protein Function Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also play an essential role during cellular mitosis and receptor capping.
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Form
Liquid
Concentration
0.2 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant Caldesmon/CDM Antibody (Ab092465) - Western Blot All lanes: Recombinant Caldesmon/CDM Antibody (Ab092465) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 93 kDa Observed band size: 76 kDa Exposure time: 9.0 s
Recombinant Caldesmon/CDM Antibody (Ab092465) - IHC Immunohistochemistry analysis of paraffin-embedded Human colon cancer using Recombinant Caldesmon/CDM Antibody (Ab092465). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
1.Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)):(4493-4501). [PMID:19413330]
2.Hayashi, K K and 8 more authors.. (1992) Genomic structure of the human caldesmon gene.. Proceedings of the National Academy of Sciences of the United States of America, (15):[PMID:1465449]
3.Humphrey, M B MB, Herrera-Sosa, H H, Gonzalez, G G, Lee, R R and Bryan, J J.. (1992) Cloning of cDNAs encoding human caldesmons.. Gene, (15):[PMID:1555769]
4.Novy, R E RE, Lin, J L JL and Lin, J J JJ.. (1991) Characterization of cDNA clones encoding a human fibroblast caldesmon isoform and analysis of caldesmon expression in normal and transformed cells.. The Journal of biological chemistry, (5):[PMID:1885618]
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6.Yu, Li-Rong LR and 5 more authors.. (2007) Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra.. Journal of proteome research, [PMID:17924679]
7.Dephoure, Noah N and 6 more authors.. (2008) A quantitative atlas of mitotic phosphorylation.. Proceedings of the National Academy of Sciences of the United States of America, (5):[PMID:18669648]
8.Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15):[PMID:21406692]
9.Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4):[PMID:23186163]
10.Bian, Yangyang Y and 9 more authors.. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.. Journal of proteomics, (16):[PMID:24275569]
11.Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460]
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