| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab092925-10μl | 10μl | In stock | $66.90 | |
| Ab092925-50μl | 50μl | In stock | $189.90 | |
| Ab092925-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| Ab092925-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,699.90 |
Recombinant; Rabbit anti Human CARD11 Antibody; WB; Unconjugated
| Product Name | Recombinant Carma 1/CARD11 Antibody - Primary antibody, specific to CARD11, Rabbit IgG |
|---|---|
| Synonyms | 0610008L17Rik antibody | 2410011D02Rik antibody | Bcl10 interacting maguk protein 3 antibody | BIMP 3 antibody | BIMP3 antibody | CAR11_HUMAN antibody | CARD 11 antibody | CARD containing MAGUK protein 3 antibody | Card maguk protein 1 antibody | CARD-con |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 1.5 mg/mL |
| Host species | Rabbit |
| Specificity | CARD11 |
| Immunogen | A synthetic peptide derived from human CARD11 (AA 1100-1154). |
| Positive Control | WB: K562 and MOLT-4 cell lysates. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human CARD11 Antibody, Recombinant, could be used for WB and so on. |
| Isotype | Rabbit IgG |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 1.5 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant Carma 1/CARD11 Antibody (Ab092925) - Western Blot
All lanes: DNAJC2/ZRF1 Antibody (Ab100171) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 133 kDa
Observed band size: 133 kDa
Exposure time: 142.0 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/2000 "> 1/500-1/2000 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0303145 | Certificate of Analysis | Mar 13, 2024 | Ab092925 |
| ZJ24F0303144 | Certificate of Analysis | Mar 13, 2024 | Ab092925 |
| 1. Scherer SW, Cheung J, MacDonald JR, Osborne LR, Nakabayashi K, Herbrick JA, Carson AR, Parker-Katiraee L, Skaug J, Khaja R et al.. (2003) Human chromosome 7: DNA sequence and biology.. Science, 300 (5620): (767-72). [PMID:12690205] [10.1021/op500134e] |
| 2. Bertin, J J and 8 more authors.. (2001) CARD11 and CARD14 are novel caspase recruitment domain (CARD)/membrane-associated guanylate kinase (MAGUK) family members that interact with BCL10 and activate NF-kappa B.. The Journal of biological chemistry, (13): [PMID:11278692] |
| 3. Gaide, O O and 5 more authors.. (2001) Carma1, a CARD-containing binding partner of Bcl10, induces Bcl10 phosphorylation and NF-kappaB activation.. FEBS letters, (11): [PMID:11356195] |
| 4. Pomerantz, Joel L JL, Denny, Elissa M EM and Baltimore, David D.. (2002) CARD11 mediates factor-specific activation of NF-kappaB by the T cell receptor complex.. The EMBO journal, (1): [PMID:12356734] |
| 5. Hillier, Ladeana W LW and 106 more authors.. (2003) The DNA sequence of human chromosome 7.. Nature, (10): [PMID:12853948] |
| 6. Ohnuma, Kei K and 10 more authors.. (2007) Caveolin-1 triggers T-cell activation via CD26 in association with CARMA1.. The Journal of biological chemistry, (30): [PMID:17287217] |
| 7. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332] |
| 8. Snow, Andrew L AL and 20 more authors.. (2012) Congenital B cell lymphocytosis explained by novel germline CARD11 mutations.. The Journal of experimental medicine, (19): [PMID:23129749] |
| 9. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
| 10. Stepensky, Polina P and 14 more authors.. (2013) Deficiency of caspase recruitment domain family, member 11 (CARD11), causes profound combined immunodeficiency in human subjects.. The Journal of allergy and clinical immunology, [PMID:23374270] |
| 11. Greil, Johann J and 11 more authors.. (2013) Whole-exome sequencing links caspase recruitment domain 11 (CARD11) inactivation to severe combined immunodeficiency.. The Journal of allergy and clinical immunology, [PMID:23561803] |
| 12. Qiao, Qi Q and 10 more authors.. (2013) Structural architecture of the CARMA1/Bcl10/MALT1 signalosome: nucleation-induced filamentous assembly.. Molecular cell, (26): [PMID:24074955] |
| 13. Jang, Tae-ho; Park, Jin Hee and Park, Hyun Ho.. (2013) Novel disulfide bond-mediated dimerization of the CARD domain was revealed by the crystal structure of CARMA1 CARD.. PloS one, [PMID:24224005] |
| 14. Yang, Yong-Kang YK and 5 more authors.. (2016) Molecular Determinants of Scaffold-induced Linear Ubiquitinylation of B Cell Lymphoma/Leukemia 10 (Bcl10) during T Cell Receptor and Oncogenic Caspase Recruitment Domain-containing Protein 11 (CARD11) Signaling.. The Journal of biological chemistry, (9): [PMID:27777308] |
| 15. Ma, Chi A CA and 38 more authors.. (2017) Germline hypomorphic CARD11 mutations in severe atopic disease.. Nature genetics, [PMID:28628108] |
| 16. Holliday, Michael J and 8 more authors.. (2019) Structures of autoinhibited and polymerized forms of CARD9 reveal mechanisms of CARD9 and CARD11 activation.. Nature communications, (11): [PMID:31296852] |
| 17. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460] |
