| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab093061-10μl | 10μl | In stock | $66.90 | |
| Ab093061-50μl | 50μl | In stock | $189.90 | |
| Ab093061-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| Ab093061-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,699.90 |
Recombinant; Rabbit anti Human Caspase-8 Antibody; WB, IHC, IF/ICC; Unconjugated
| Product Name | Recombinant Caspase-8 Antibody - Primary antibody, specific to CASP8, Rabbit IgG |
|---|---|
| Synonyms | ALPS2B antibody | Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 proteinantibody | Apoptosis related cysteine peptidase antibody | Apoptotic cysteine protease antibody | Apoptotic protease Mch-5 antibody | Apoptotic protease Mch5 ant |
| Specifications & Purity | Recombinant, ExactAb™, Validated, Lot by Lot |
| Biochemical and Physiological Mechanisms | Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signa |
| Host species | Rabbit |
| Specificity | CASP8 |
| Immunogen | A synthetic peptide derived from human Caspase-8 (AA 385-435) |
| Positive Control | WB: A431, K562, A549 and THP-1 cell lysates. IHC: Human uterus tissue. ICC/IF: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human Caspase-8 Antibody, Recombinant, could be used for WB, IHC, IF/ICC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant Caspase-8 Antibody (Ab093061) - Western Blot
All lanes: Recombinant Caspase-8 Antibody (Ab093061) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 55 kDa
Observed band size: 54, 57 kDa
Exposure time: 47.3 s
Recombinant Caspase-8 Antibody (Ab093061) - Western Blot
All lanes: Recombinant Caspase-8 Antibody (Ab093061) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 55 kDa
Observed band size: 54, 57 kDa
Exposure time: 20.0 s
Recombinant Caspase-8 Antibody (Ab093061) - IF/ICC
Immunofluorescence analysis of Caspase 8 in HeLa using Recombinant Caspase-8 Antibody (Ab093061).
Recombinant Caspase-8 Antibody (Ab093061) - IHC
Immunohistochemistry analysis of paraffin-embedded Human uterus using Recombinant Caspase-8 Antibody (Ab093061). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
| Application | Dilution info |
|---|---|
| IHC | 1/100 - 1/200 "> 1/100 - 1/200 |
| IF/ICC |
1/50-1/200
">
1/50-1/200
|
| WB | 1/1000 - 1/2000"> 1/1000 - 1/2000 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0202956 | Certificate of Analysis | Mar 01, 2024 | Ab093061 |
| ZJ24F0202955 | Certificate of Analysis | Mar 01, 2024 | Ab093061 |
