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Recombinant CD133 Antibody - Primary antibody, specific to CD133, Rabbit IgG

  • ExactAb™
  • Recombinant
  • Validated
  • 0.6 mg/mL
Features and benefits
  • Short Overview:

    Recombinant; Rabbit anti Human CD133 Antibody; WB; Unconjugated

  • Species reactivity(Reacts with): Human
  • Isotype: Rabbit IgG
    Application:
  • WB
Item Number
Ab093819
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab093819-10μl
10μl
In stock
$66.90
Ab093819-50μl
50μl
In stock
$189.90
Ab093819-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$299.90
Ab093819-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,809.90

Recombinant; Rabbit anti Human CD133 Antibody; WB; Unconjugated

Basic Description

Product NameRecombinant CD133 Antibody - Primary antibody, specific to CD133, Rabbit IgG
SynonymsAC133 antibody; Antigen AC133 antibody; CD133 antibody; CORD12 antibody; Hematopoietic stem cell antigen antibody; hProminin antibody; MCDR2 antibody; MSTP061 antibody; OTTHUMP00000217744 antibody; OTTHUMP00000217745 antibody; OTTHUMP00000217746 antibody;
Specifications & PurityExactAb™, Validated, Recombinant, 0.6 mg/mL
Host speciesRabbit
SpecificityCD133
ImmunogenA synthetic peptide derived from human CD133 (AA 250-380).
Positive ControlWB: HT-29 lysates.
ConjugationUnconjugated
GradeExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human CD133 Antibody, Recombinant, could be used for WB and so on.
Application:
WB: 1/500-1/2000
Protein Function:
May play a role in cell differentiation, proliferation and apoptosis (PubMed:24556617). Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner (PubMed:20818439).

Product Properties

IsotypeRabbit IgG
Light Chain Typekappa
SDS-PAGE150 kDa
Purification MethodProtein A purified
FormLiquid
Concentration0.6 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant CD133 Antibody (Ab093819) - Western Blot
All lanes: Recombinant CD133 Antibody (Ab093819) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution

Predicted band size: 97 kDa
Observed band size: 120 kDa
Exposure time: 100.0 s

Associated Targets

PROM1 Tbio Prominin-1 0 Activities

Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
WB

1/500-1/2000

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section

2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0303149Certificate of AnalysisMar 13, 2024 Ab093819
ZJ24F0303148Certificate of AnalysisMar 13, 2024 Ab093819

Related Documents

References

1. Yin, A H AH and 8 more authors..  (1997)  AC133, a novel marker for human hematopoietic stem and progenitor cells..  Blood,    (15):   [PMID:9389720]
2. Miraglia, S S and 8 more authors..  (1997)  A novel five-transmembrane hematopoietic stem cell antigen: isolation, characterization, and molecular cloning..  Blood,    (15):   [PMID:9389721]
3. Maw, M A MA and 12 more authors..  (2000)  A frameshift mutation in prominin (mouse)-like 1 causes human retinal degeneration..  Human molecular genetics,    (1):   [PMID:10587575]
4. Yu, Ying Y, Flint, Alan A, Dvorin, Evan L EL and Bischoff, Joyce J..  (2002)  AC133-2, a novel isoform of human AC133 stem cell antigen..  The Journal of biological chemistry,    (7):   [PMID:12042327]
5. Hillier, Ladeana W LW and 121 more authors..  (2005)  Generation and annotation of the DNA sequences of human chromosomes 2 and 4..  Nature,    (7):   [PMID:15815621]
6. Fargeas, C A CA, Huttner, W B WB and Corbeil, D D..  (2007)  Nomenclature of prominin-1 (CD133) splice variants - an update..  Tissue antigens,      [PMID:17498271]
7. Zhang, Qingjiong Q and 9 more authors..  (2007)  Severe retinitis pigmentosa mapped to 4p15 and associated with a novel mutation in the PROM1 gene..  Human genetics,      [PMID:17605048]
8. Huttner, Hagen B HB and 20 more authors..  (2008)  The stem cell marker prominin-1/CD133 on membrane particles in human cerebrospinal fluid offers novel approaches for studying central nervous system disease..  Stem cells (Dayton, Ohio),      [PMID:18096722]
9. Yang, Zhenglin Z and 32 more authors..  (2008)  Mutant prominin 1 found in patients with macular degeneration disrupts photoreceptor disk morphogenesis in mice..  The Journal of clinical investigation,      [PMID:18654668]
10. Takenobu, H H and 7 more authors..  (2011)  CD133 suppresses neuroblastoma cell differentiation via signal pathway modification..  Oncogene,    (6):   [PMID:20818439]
11. Rigbolt, Kristoffer T G KT and 9 more authors..  (2011)  System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation..  Science signaling,    (15):   [PMID:21406692]
12. Mak, Anthony B AB and 6 more authors..  (2014)  Post-translational regulation of CD133 by ATase1/ATase2-mediated lysine acetylation..  Journal of molecular biology,    (29):   [PMID:24556617]

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