A synthetic peptide derived from human CD133 (AA 250-380).
Positive Control
WB: HT-29 lysates.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human CD133 Antibody, Recombinant, could be used for WB and so on. Application: WB: 1/500-1/2000 Protein Function: May play a role in cell differentiation, proliferation and apoptosis (PubMed:24556617). Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner (PubMed:20818439).
Product Properties
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Form
Liquid
Concentration
0.6 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant CD133 Antibody (Ab093819) - Western Blot All lanes: Recombinant CD133 Antibody (Ab093819) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 97 kDa Observed band size: 120 kDa Exposure time: 100.0 s
1.Yin, A H AH and 8 more authors.. (1997) AC133, a novel marker for human hematopoietic stem and progenitor cells.. Blood, (15):[PMID:9389720]
2.Miraglia, S S and 8 more authors.. (1997) A novel five-transmembrane hematopoietic stem cell antigen: isolation, characterization, and molecular cloning.. Blood, (15):[PMID:9389721]
3.Maw, M A MA and 12 more authors.. (2000) A frameshift mutation in prominin (mouse)-like 1 causes human retinal degeneration.. Human molecular genetics, (1):[PMID:10587575]
4.Yu, Ying Y, Flint, Alan A, Dvorin, Evan L EL and Bischoff, Joyce J.. (2002) AC133-2, a novel isoform of human AC133 stem cell antigen.. The Journal of biological chemistry, (7):[PMID:12042327]
5.Hillier, Ladeana W LW and 121 more authors.. (2005) Generation and annotation of the DNA sequences of human chromosomes 2 and 4.. Nature, (7):[PMID:15815621]
6.Fargeas, C A CA, Huttner, W B WB and Corbeil, D D.. (2007) Nomenclature of prominin-1 (CD133) splice variants - an update.. Tissue antigens, [PMID:17498271]
7.Zhang, Qingjiong Q and 9 more authors.. (2007) Severe retinitis pigmentosa mapped to 4p15 and associated with a novel mutation in the PROM1 gene.. Human genetics, [PMID:17605048]
8.Huttner, Hagen B HB and 20 more authors.. (2008) The stem cell marker prominin-1/CD133 on membrane particles in human cerebrospinal fluid offers novel approaches for studying central nervous system disease.. Stem cells (Dayton, Ohio), [PMID:18096722]
9.Yang, Zhenglin Z and 32 more authors.. (2008) Mutant prominin 1 found in patients with macular degeneration disrupts photoreceptor disk morphogenesis in mice.. The Journal of clinical investigation, [PMID:18654668]
10.Takenobu, H H and 7 more authors.. (2011) CD133 suppresses neuroblastoma cell differentiation via signal pathway modification.. Oncogene, (6):[PMID:20818439]
11.Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15):[PMID:21406692]
12.Mak, Anthony B AB and 6 more authors.. (2014) Post-translational regulation of CD133 by ATase1/ATase2-mediated lysine acetylation.. Journal of molecular biology, (29):[PMID:24556617]
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